{"product_id":"dna-ligation-kit-mighty-mix-6023","title":"DNA Ligation Kit, Mighty Mix","description":"\u003cp\u003eTakara's DNA Ligation Kit, Mighty Mix, is a one-solution premix ligation reagent that offers high efficiency ligations, particularly for blunt-ended ligation. The reaction can be performed for 5 minutes at 25°C (rapid protocol), or 30 minutes at 16°C (standard protocol) with equal results for both circular and linear DNA ligations. The 2X Ligation Mix allows small ligation reaction volumes (10 μl) when DNA is limiting. The kit includes sufficient reagent for 100 ligation reactions. Ligation products reaction can be used directly in bacterial transformation.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEnables high-efficiency ligations for cohesive, blunt-end, and TA cloning\u003c\/li\u003e\n\u003cli\u003e2X ligation mix adds convenience and eliminates extra pipetting and purification steps\u003c\/li\u003e\n\u003cli\u003eLigation reactions can be quickly performed at either 5 or 30 minutes depending on DNA type\u003c\/li\u003e\n\u003cli\u003eReaction volumes are scalable\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHighly efficient and fast DNA ligation\u003cbr\u003eSmall reaction volume ligation\u003cbr\u003eTA and blunt-end cloning\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/6023\/6023.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0938\/SD0938-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/6023_Protocol\/6023_Protocol-at-a-Glance_1212.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"100 Rxns","offer_id":45986007253071,"sku":"6023","price":1059.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/6023-DNA-Ligation-Kit-Mighty-Mix-product-600.jpg?v=1782801651","url":"https:\/\/www.danyel.co.il\/products\/dna-ligation-kit-mighty-mix-6023","provider":"danyel biotech","version":"1.0","type":"link"}