{"product_id":"dispase-ii-neutral-protease-grade-ii-tbs2117-01","title":"Dispase II (Neutral Protease, Grade II)","description":"\u003cp\u003eDispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It can be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. It can also be used to prevent clumping in suspension cultures. This protease cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ and Al3+ activate the enzyme. EDTA, EGTA, Hg2+, and other heavy metals inhibit enzyme activity.\u003c\/p\u003e\n\u003cp\u003eTribioscience’s Dispase II is a lyophilized powder, and stable at 2-8°C. It makes it easy to storage and application.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eMain Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRapid, effective, yet gentle agent that liberates cells with minimal cell damage.\u003c\/li\u003e\n\u003cli\u003eMaintains cell membrane integrity.\u003c\/li\u003e\n\u003cli\u003eNon-mammalian source – free of mycoplasma and animal virus contamination.\u003c\/li\u003e\n\u003cli\u003eExtremely stable to influences of temperature, pH, and interference by serum components.\u003c\/li\u003e\n\u003cli\u003eEasily inactivated by chelating agents or by dilution.\u003c\/li\u003e\n\u003cli\u003eDelivers higher activity and convenience.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eDispase II is stored at +2 to +8°C for a shelf-life of 1 year. The expiration date is printed on the label.\u003cbr\u003e\n\u003c!--TBS2117-01--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/11\/TBS2117_dispase-II_data-sheet_V01_2022.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2025\/12\/TBS2117-dispase-II_data-sheet_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003e1. Wu, H. F., Saito-Diaz, K., Huang, C. W., McAlpine, J. L., Seo, D. E., Magruder, D. S., Ishan, M., Bergeron, H. C., Delaney, W. H., Santori, F. R., Krishnaswamy, S., Hart, G. W., Chen, Y. W., Hogan, R. J., Liu, H. X., Ivanova, N. B., \u0026amp; Zeltner, N. (2024). Parasympathetic neurons derived from human pluripotent stem cells model human diseases and development. \u003ci\u003eCell stem cell\u003c\/i\u003e, \u003ci\u003e31\u003c\/i\u003e(5), 734–753.e8. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.stem.2024.03.011\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1016\/j.stem.2024.03.011\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e2. Wu, H. F., Ishan, M., Rashid, M. M., Liu, H. X., \u0026amp; Zeltner, N. (2024). Novel Cross-Species Salivary Gland-Parasympathetic Neuron Coculture System. Bio-protocol, 14(21), e5101. \u003ca href=\"https:\/\/doi.org\/10.21769\/BioProtoc.5101\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.21769\/BioProtoc.5101\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986455191631,"sku":"TBS2117-01","price":1270.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2117.jpg?v=1782811287","url":"https:\/\/www.danyel.co.il\/products\/dispase-ii-neutral-protease-grade-ii-tbs2117-01","provider":"danyel biotech","version":"1.0","type":"link"}