{"product_id":"dextrin-sepharose-high-performance-mbp-tagged-protein-purification-resin-28935598","title":"Dextrin Sepharose™ High Performance MBP-tagged protein purification resin","description":"\u003cp\u003eDextrin Sepharose High Performance is a robust and stable affinity resin for purification of proteins tagged with maltose binding protein (MBP).\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFast, one-step purification of MBP-tagged proteins.\u003c\/li\u003e\n\u003cli\u003eHigh-resolution purification and elution in narrow peaks, minimizing the need for further concentration steps.\u003c\/li\u003e\n\u003cli\u003eCan be used for repeated purification: can be easily regenerated using 0.5 M NaOH.\u003c\/li\u003e\n\u003cli\u003ePhysiological conditions and mild elution preserve target protein activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003ePurifying proteins tagged with maltose binding protein\u003c\/p\u003e\u003cp\u003eRecombinant proteins are engineered with an MBP-tag to facilitate detection, isolation, and purification procedures. In addition, this affinity tag is often chosen due to its ability to increase the expression level and solubility of the fusion protein.\u003c\/p\u003e\u003cp\u003ePurification of recombinant proteins is done under physiological conditions, which together with mild elution by maltose, preserves the activity of the target protein.\u003c\/p\u003e\u003cp\u003eDextrin Sepharose High Performance MBP resin\u003c\/p\u003e\u003cp\u003eThe small, evenly sized beads ensure that tagged proteins elute in narrow peaks, thus minimizing the need for further concentration steps. The resin is easily regenerated using 0.5 M NaOH, allowing the same column to be used to purify different proteins.\u003c\/p\u003e\u003cp\u003eSuccessful use in published research\u003c\/p\u003e\u003cp\u003eResearch and applications using MBP resin include:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003ePurifying tagged \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/22961982\/\"\u003eArabidopsis Deg2 proteins to examine their crystal structure\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePurifying recombinant proteins to \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27149614\/\"\u003estudy RNA editing in Arabidopsis thaliana mitochondria\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCompatible empty columns\u003c\/p\u003e\u003cp\u003eCytiva's solutions include a variety of empty columns. Dextrin Sepharose High Performance resin is compatible with Tricorn, XK, and HiScale column lines.\u003c\/p\u003e\u003cp\u003eConvenient prepacked columns\u003c\/p\u003e\u003cp\u003eThis resin is also available as prepacked MBP-Trap columns. These HiTrap format columns can be used with a syringe, a pump, or a chromatography system.\u003c\/p\u003e\u003cp\u003eOther Cytiva products for affinity purification\u003c\/p\u003e\u003cp\u003eLooking for a different affinity resin? Let Cytiva help you find the right product to purify your tagged recombinant protein.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-15011-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-14280-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"25 mL","offer_id":45982368006223,"sku":"28935597","price":6248.0,"currency_code":"ILS","in_stock":true},{"title":"100 mL","offer_id":45982368038991,"sku":"28935598","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7786-original-png_6b74cf49-339b-4c72-84ef-e8afac236d55.png?v=1785960666","url":"https:\/\/www.danyel.co.il\/products\/dextrin-sepharose-high-performance-mbp-tagged-protein-purification-resin-28935598","provider":"danyel biotech","version":"1.0","type":"link"}