{"product_id":"deoxyribonuclease-enzyme-activity-tbp0066","title":"Deoxyribonuclease, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 200 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 90%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe amount of enzyme which results in an increase in absorbance at 260 nm and 0.001 per minute per ml at 25°C at pH 5.00.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe assay method is a modification of the method of Kunitz. An increase in absorbance at 260 nm, caused by the depolymerization of DNA by the enzyme, is a measure of enzyme activity.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eDeoxyribonuclease (DNase I) (EC 3.1.4.5), from bovine pancreas, is an endonuclease catalyzing the hydrolysis of phosphodiester linkages, adjacent to a pyrimidine nucleotide yielding 5’phosphate terminated polynucleotides. The enzyme is able to act upon single as well as double-stranded DNA and on chromatin.\u003c\/p\u003e\n\u003cp\u003eThe DNase from bovine pancreas exists in multiple forms with DNase A being the predominant form. In addition to bovine pancreas, DNase activity has also been demonstrated in other mammalian tissues.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e1.00 M Acetate Buffer, pH 5.00.\u003c\/li\u003e\n\u003cli\u003e0.05 M Magnesium sulfate.\u003c\/li\u003e\n\u003cli\u003eSubstrate: 4 mg DNA\/100 ml solution. (Dissolve 4 mg of highly polymerized DNA in 50 ml of distilled water, add 10 ml acetate buffer and 10 ml magnesium sulfate. Dilute to 100 ml with distilled water.) The substrate will be stable for 2 weeks if stored at 4°C.\u003c\/li\u003e\n\u003cli\u003eEnzyme – dissolved in acetate buffer to yield a final concentration of 40-60 U\/ml.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet spectrophotometer (equipped with a strip chart recorder and temperature control) at 260 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eIn quartz cuvettes pipette the following at 25°C:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003e                                    TEST               CONTROL\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eSubstrate                    3.0 ml                  3.0 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eDistilled water                                         1.0 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eEnzyme @ zero time  1.0 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eRecord the rate of increase in absorbance at 260 nm for approximately 8 min. after the initial lag time of approximately 15 min.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBP0066-1G TBP0066-5G--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0066-Deoxyribonuclease-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0066_Deoxyribonuclease-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 g","offer_id":45986498674767,"sku":"TBP0066-1G","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 g","offer_id":45986498707535,"sku":"TBP0066-5G","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0066.png?v=1782812640","url":"https:\/\/www.danyel.co.il\/products\/deoxyribonuclease-enzyme-activity-tbp0066","provider":"danyel biotech","version":"1.0","type":"link"}