{"product_id":"deaza-dgtp-27209002","title":"Deaza dGTP","description":"\u003cp\u003eDeaza dGTP is a nucleotide analog that is a candidate substrate for processive telomerase. Its use can improve Polymerase Chain Reaction (PCR) yields and makes sequencing of GC rich sequences easier.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor reducing band compressions* in sequencing gels.\u003c\/li\u003e\n\u003cli\u003eSupplied as convenient solutions at pH 7.0 and 7.5. c⁷dGTP is supplied in purified water as 5 mM and 10 mM solutions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eDeaza dGTP is a substrate for most DNA polymerases, including \u003ca href=\"\/shop\/molecular-biology\/pcr-and-amplification\/enzymes\/taq-dna-polymerase-cloned-p-00704\"\u003eTaq DNA polymerase\u003c\/a\u003e. It can also be applied to dideoxy-chain termination sequencing methods, in place of dGTP to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA. Partial substitution of Deaza dGTP for dGTP in PCR can improve the product yield when the template is GC-rich.\u003c\/p\u003e\n\n\u003cp\u003eTechnical Information:\u003c\/p\u003e\n\n\u003cp\u003e*Band compressions are ambiguous band patterns on DNA sequencing gels. This is caused by stable, intra-strand secondary structures which are not fully denatured during electrophoresis. The likelihood of occurrence can be reduced through substitution of nucleotide analogs within sequencing mixers. To resolve G-band compressions, use 7-deaza dGTP (c⁷dGTP).\u003c\/p\u003e","brand":"Cytiva","offers":[{"title":"2 µmoL","offer_id":45982217076815,"sku":"27209002","price":1916.0,"currency_code":"ILS","in_stock":true}],"url":"https:\/\/www.danyel.co.il\/products\/deaza-dgtp-27209002","provider":"danyel biotech","version":"1.0","type":"link"}