{"product_id":"deae-sepharose-fast-flow-anion-exchange-chromatography-resin-17070901","title":"DEAE Sepharose™ Fast Flow anion exchange chromatography resin","description":"\u003cp\u003eDEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eWell-proven weak anion exchanger developed for industrial downstream processes.\u003c\/li\u003e\n\u003cli\u003eUsed extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals.\u003c\/li\u003e\n\u003cli\u003eHigh chemical stability allows for well proven CIP and sanitization protocols.\u003c\/li\u003e\n\u003cli\u003eHydrophilic base matrix ensures low levels of non-specific binding, and low levels of host cell-derived impurities in the elution pool.\u003c\/li\u003e\n\u003cli\u003eFulfills industrial demands for security of supply, robust performance, and regulatory support.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStreamlined scale-up\u003c\/p\u003e\u003cp\u003eThis resin is part of the Sepharose Fast Flow ion exchange platform, which is well established in industrial downstream processing. Composed of crosslinked 6% agarose beads with diethylaminoethyl (DEAE) weak anion exchange groups, the resin has high chemical stability, allowing well-proven cleaning-in-place (CIP) and sanitization protocols.\u003c\/p\u003e\u003cp\u003eThe highly crosslinked agarose resin enables high flow rates and high productivity. Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, and PreDictor RoboColumns™, and HiTrap columns.\u003c\/p\u003e\u003cp\u003eSuccessful use – research scale\u003c\/p\u003e\u003cp\u003eDEAE Sepharose Fast Flow resin is used in a variety of research applications, including:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eSeparation of\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/8106399\/\"\u003ePI 3-Kinases from guinea pig neutrophils\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003ePurification of \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/7982954\/\"\u003emyosin phosphatase from chicken smooth muscle\u003c\/a\u003e \u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCompatible empty columns – research scale\u003c\/p\u003e\u003cp\u003eCytiva's solutions include a variety of empty columns for research scale. This DEAE resin is compatible with two of these lines, Tricorn and XK columns.\u003c\/p\u003e\u003cp\u003eCompatible empty columns – bioprocess scale\u003c\/p\u003e\u003cp\u003eFor process-scale applications for this DEAE resin, Cytiva recommends AxiChrom columns. DEAE Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.\u003c\/p\u003e\u003cp\u003ePrepacked columns – research scale\u003c\/p\u003e\u003cp\u003eThis weak anion exchange resin is available in the following formats: \n\u003c\/p\u003e\u003cul\u003e\u003cli\u003e\u003chitrap deae ff convenient for easy use with a syringe peristaltic pump or chromatography system\u003e\u003cli\u003eHiScreen DEAE FF for scale up and process development\u003c\/li\u003e\n\u003cli\u003eHiPrep DEAE FF columns for convenient scale-up\u003c\/li\u003e\u003c\/hitrap\u003e\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003ePrepacked columns – bioprocess scale\u003c\/p\u003e\u003cp\u003ePrepacked ReadyToProcess columns for bioprocess applications are available on request.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-12944-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/71500964.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"10 L","offer_id":45982042751055,"sku":"17070905","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"25 mL","offer_id":45982042783823,"sku":"17070910","price":499.0,"currency_code":"ILS","in_stock":true},{"title":"500 mL","offer_id":45982042816591,"sku":"17070901","price":3829.0,"currency_code":"ILS","in_stock":true},{"title":"Standard","offer_id":45982042849359,"sku":"17070960","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-4547-original-png_f436864c-10fb-4d9f-b8b0-8e2d7ecd24ea.png?v=1785957647","url":"https:\/\/www.danyel.co.il\/products\/deae-sepharose-fast-flow-anion-exchange-chromatography-resin-17070901","provider":"danyel biotech","version":"1.0","type":"link"}