{"product_id":"d-3-hydroxybutyrate-dehydrogenase-enzyme-activity-tbp0027","title":"D-3-Hydroxybutyrate dehydrogenase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003ePreparation and Specification\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eAppearance:\u003c\/u\u003e White amorphous powder, lyophilized\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e GradeⅢ 100U\/mg-solid or more\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eContaminants:\u003c\/u\u003e Malate dehydrogenase ≤2.0×10⁻³%\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px\"\u003eLactate dehydrogenase ≤2.0×10⁻³%\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px\"\u003eNADH oxidase ≤2.0×10⁻³%\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStabilizers:\u003c\/u\u003e Sucrose, mannitol, BSA\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProperties\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Stable at -20°C for at least Two years\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular weight:\u003c\/u\u003e approx. 130,000 (by gel filtration)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eIsoelectric point:\u003c\/u\u003e 5.6±0.1\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMichaelis constants:\u003c\/u\u003e 4.2×10⁻⁴M (25°C, pH8.3), 7.0×10⁻⁴M(37°C, pH8.3)(D-3-Hydroxybutyrate)\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px\"\u003e4.9×10⁻⁵M (25°C, pH8.3), 7.2×10⁻⁵M (37°C, pH8.3)(NAD⁺)\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px\"\u003e8.1×10⁻⁵M (25°C, pH7.1), 2.4×10⁻⁴M (37°C, pH7.1)(Acetoacetate)\u003c\/p\u003e\n\u003cp style=\"padding-left: 80px\"\u003e8.4×10⁻⁶M (25°C, pH7.1), 1.5×10⁻⁵M (37°C, pH7.1)(NADH)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eInhibitors:\u003c\/u\u003e PCMB, MIA, IAA, Ag⁺, Hg⁺⁺, SDS, DAC\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eOptimum pH:\u003c\/u\u003e 8.3\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eOptimum temperature:\u003c\/u\u003e 55°C\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003epH Stability:\u003c\/u\u003e pH 5.0-8.5 (25°C, 20hr)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eThermal stability:\u003c\/u\u003e below 40°C (pH 6.5, 15min)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis enzyme is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBP0027-100U TBP0027-500U--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/02\/TBP0027-D-3-Hydroxybutyrate-dehydrogenase.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/02\/TBP0027_D-3-Hydroxybutyrate-dehydrogenase-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"100U","offer_id":45986494611535,"sku":"TBP0027-100U","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"500U","offer_id":45986494644303,"sku":"TBP0027-500U","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0027.png?v=1782812561","url":"https:\/\/www.danyel.co.il\/products\/d-3-hydroxybutyrate-dehydrogenase-enzyme-activity-tbp0027","provider":"danyel biotech","version":"1.0","type":"link"}