{"product_id":"cytochrome-c-reductase-activity-assay-tbs2116","title":"Cytochrome C Reductase Activity Assay","description":"\u003cp\u003eThis kit is designed to measure the NADPH cytochrome C reductase activity in biological samples including cell and tissue extracts, and in purified microsomes (endoplasmic reticulum, ER). Eukaryotic NADPH-Cytochrome C reductase (NADPH cytochrome P450 reductase, EC 1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which is the cytochrome P450 family of enzymes, responsible for xenobiotic metabolism.\u003c\/p\u003e\n\u003cp\u003eNADPH-cytochrome C reductase is widely used as an endoplasmic reticulum marker and as a biomarker of ecological pollution and dietary lipid uptake. This kit is based on a colorimetric assay that measures the reduction of cytochrome C by NADPH-Cytochrome C reductase in the presence of NADPH. The reduction of cytochrome C results in the formation of distinct bands in the absorption spectrum and the increase in absorbance at 550 nm is measured with time.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMeasuring NADPH-cytochrome c reductase levels in biological samples after exposure to drugs or other xenobiotics.\u003c\/li\u003e\n\u003cli\u003eDetection of ER during isolation and subcellular fractionation by density gradient separation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKey Features\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlexible:\u003c\/strong\u003e The kit can be used for 96-well plates or 384-well plates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAccurate:\u003c\/strong\u003e Accurate detection of cytochrome C reductase activity in small size as 50 µL samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSimple:\u003c\/strong\u003e Just load-Read. The kit can be used for a robust method.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTime-saving:\u003c\/strong\u003e Less than 30 minutes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Contents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 363px;height: 146px\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Buffer\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCytochrome C\u003c\/td\u003e\n\u003ctd\u003e1.2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNADPH\u003c\/td\u003e\n\u003ctd\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCytochrome C Reductase Positive\u003c\/td\u003e\n\u003ctd\u003e0.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCytochrome C Oxidase Inhibitor\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore kit at -20°C. Shelf life of 2 years. Protect from light.\u003cbr\u003e\nShip with blue ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Products\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/resazurin-cell-viability-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eCell Viability Assay Kits (TBS2001)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/wst-8-or-cell-count-kit-8cck-8\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eCCK-8 Cell Viability (TBS2022)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/atp-colorimetric-fluorometric-assay-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eATP Colorimetric\/Fluorometric Assay (TBS2010)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/adp-colorimetric-fluorometric-assay-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eADP Colorimetric\/Fluorometric Assay Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/fast-glucose-oxidase-activity-assay-colorimetric-fluorometric\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eGlucose Oxidase Colorimetric\/Fluorometric Assay (TBS2088)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS2116--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS2116_v02_2023.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2026\/04\/TBS2116-Cytochrome-C-Reductase-Activity-Assay_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003e1. Sierra, T., \u0026amp; Achour, B. (2024). In Vitro to In Vivo Scalars for Drug Clearance in Nonalcoholic Fatty Liver and Steatohepatitis. Drug metabolism and disposition: the biological fate of chemicals, 52(5), 390–398. \u003ca href=\"https:\/\/doi.org\/10.1124\/dmd.123.001629\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1124\/dmd.123.001629\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e2. Lin, Y., Hou, X., Shen, W. J., Hanssen, R., Khor, V. K., Cortez, Y., Roseman, A. N., Azhar, S., \u0026amp; Kraemer, F. B. (2016). SNARE-Mediated Cholesterol Movement to Mitochondria Supports Steroidogenesis in Rodent Cells. \u003cem\u003eMolecular endocrinology (Baltimore, Md.)\u003c\/em\u003e, \u003cem\u003e30\u003c\/em\u003e(2), 234–247. \u003ca href=\"https:\/\/doi.org\/10.1210\/me.2015-1281\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1210\/me.2015-1281\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986454896719,"sku":"TBS2116","price":1913.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2116.jpg?v=1782811285","url":"https:\/\/www.danyel.co.il\/products\/cytochrome-c-reductase-activity-assay-tbs2116","provider":"danyel biotech","version":"1.0","type":"link"}