{"product_id":"cytochrome-c-oxidase-activity-assay-tbs2115","title":"Cytochrome C Oxidase Activity Assay","description":"\u003cp\u003eCytochrome c oxidase [EC 1.9.3.1.] or Complex IV is the fourth complex of the electron Transport Chain located in the mitochondrial, and the principal terminal oxidase of high-affinity oxygen in the aerobic metabolism of all animals, plants, yeasts, and some bacteria. This enzyme is probably unique in providing energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.\u003c\/p\u003e\n\u003cp\u003eThe colorimetric assay in this kit is based on the observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. It is suitable for the detection of mitochondrial outer membrane integrity and for the detection of mitochondria in subcellular fractions.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComponents and Storage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit is sufficient for 100 tests. Storage at –20°C\u003c\/p\u003e\n\u003ctable style=\"height: 205px\" border=\"1px solid black\" width=\"406px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"192\"\u003ePart Name\u003c\/td\u003e\n\u003ctd width=\"75\"\u003ePart Size\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"192\"\u003eAssay Buffer, 5 x\u003c\/td\u003e\n\u003ctd width=\"75\"\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"192\"\u003eEnzyme Dilution Buffer, 2x\u003c\/td\u003e\n\u003ctd width=\"75\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"192\"\u003eCytochrome C Solution\u003c\/td\u003e\n\u003ctd width=\"75\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"192\"\u003eDithiothreitol (DTT), 1M\u003c\/td\u003e\n\u003ctd width=\"75\"\u003e30 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"192\"\u003e\n\u003cem\u003en\u003c\/em\u003e-Dodecyl β-D-maltoside Buffer\u003c\/td\u003e\n\u003ctd width=\"75\"\u003e2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"192\"\u003eCytochrome c Oxidase Positive Control\u003c\/td\u003e\n\u003ctd width=\"75\"\u003e50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe kit ships on wet ice and storage at –20°C is recommended. When stored unopened, the components in this kit are stable for 24 months. After the initial thawing of the 1 M Dithiothreitol Solution, divide the solution into undiluted working aliquots (still at 1 M concentration) and store at -20°C.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS2115--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS2115_COX_Assay_v04_96wellplate_2023.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS2115-COX-Assay_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003e1. Hidayat, M., Khaliq, S., Khurram, A., \u0026amp; Lone, K. P. (2019). Protective effects of melatonin on mitochondrial injury and neonatal neuron apoptosis induced by maternal hypothyroidism. \u003cem\u003eMelatonin Research\u003c\/em\u003e, 2(4):42-60. \u003ca href=\"https:\/\/www.researchgate.net\/profile\/Abdullah-Khurram\/publication\/338007939_Protective_effects_of_melatonin_on_mitochondrial_injury_and_neonatal_neuron_apoptosis_induced_by_maternal_hypothyroidism\/links\/5dfa15444585159aa4850cc5\/Protective-effects-of-melatonin-on-mitochondrial-injury-and-neonatal-neuron-apoptosis-induced-by-maternal-hypothyroidism.pdf\" target=\"_blank\" rel=\"noopener\"\u003eDOI:10.32794\/mr11250040\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e2. Lin, Y., Hou, X., Shen, W. J., Hanssen, R., Khor, V. K., Cortez, Y., Roseman, A. N., Azhar, S., \u0026amp; Kraemer, F. B. (2016). SNARE-Mediated Cholesterol Movement to Mitochondria Supports Steroidogenesis in Rodent Cells. \u003cem\u003eMolecular endocrinology (Baltimore, Md.)\u003c\/em\u003e, \u003cem\u003e30\u003c\/em\u003e(2), 234–247. \u003ca href=\"https:\/\/doi.org\/10.1210\/me.2015-1281\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1210\/me.2015-1281\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986454831183,"sku":"TBS2115","price":1913.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2115.jpg?v=1782811285","url":"https:\/\/www.danyel.co.il\/products\/cytochrome-c-oxidase-activity-assay-tbs2115","provider":"danyel biotech","version":"1.0","type":"link"}