{"product_id":"cydye-post-labeling-reactive-dye-packs-rpn5661","title":"CyDye Post-Labeling Reactive Dye Packs","description":"\u003cp\u003eCyDye Post-Labeling Reactive Dye Pack for the generation of highly fluorescent Cy3- and Cy5-labeled probes via post-labeling (amino allyl) method.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eUses Reactive CyDye fluorescent dyes: optimized for microarray probe labeling\u003c\/li\u003e\n\u003cli\u003eProvides conveniently packaged, highly reactive Cy3 and Cy5 monofunctional NHS ester: to label probes via the post-labeling (amino allyl) route for use in microarray applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eA two-step protocol to prepare cDNA probes labeled with CyDye fluorescent dyes\u003c\/p\u003e\n\n\u003cp\u003eIn the first step, reverse transcriptase synthesizes first-strand cDNA, incorporating a chemically reactive nucleotide analog (amino allyl-dUTP) into the cDNA. The mRNA template is then degraded and the cDNA purified to remove free nucleotides and primers. In the second step, the purified cDNA is post-labeled with the reactive forms of Cy3 or Cy5 NHS esters that bind to the modified amino allyl-nucleotides. After a final purification, the labeled cDNA is ready for hybridization.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/RPN5661PAF.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"12 x Cy3 12 x Cy5","offer_id":45982271373391,"sku":"RPN5661","price":4244.0,"currency_code":"ILS","in_stock":true}],"url":"https:\/\/www.danyel.co.il\/products\/cydye-post-labeling-reactive-dye-packs-rpn5661","provider":"danyel biotech","version":"1.0","type":"link"}