{"product_id":"cyclic-amp-colorimetric-assay-tbs2073-100","title":"Cyclic AMP Colorimetric Assay","description":"\u003cp\u003eThis assay kit is a competition enzyme-linked immunoassay designed to measure cAMP levels in cells or tissues. An anti-mouse IgG was pre-coated onto a microplate. The cAMP in the test sample competes with cAMP-HRP for binding to an anti-cAMP mouse monoclonal antibody. Following the incubation and wash step, an ultra-sensitive substrate TMB is added for color development. Because of the competitive nature of this assay, the color intensity is inversely proportional to the quantity of sample cAMP levels. Measurement of absorbance using the cAMP Standard allows calculating the absolute amount of cAMP in a sample. This assay provides a flexible, accurate, simple, and time-saving approach for cAMP detection.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 2 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of cAMP is typically 1 nM.\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV and the Inter-assay CV are ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes cAMP. No cross-reactivity with others.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStandard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-25144 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/12\/TBS2073-2.png\" alt=\"\" width=\"610\" height=\"333\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStandard Curve (Fig.2)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-25146 size-medium\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/12\/TBS2073-3-1-300x209.png\" alt=\"\" width=\"300\" height=\"209\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"1115\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrecoated Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS2073A\u003c\/td\u003e\n\u003ctd\u003e96 well polystyrene microplate (12 strips of 8 wells) coated with anti-mouse IgG antibody.\u003c\/td\u003e\n\u003ctd\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ecAMP Standard\u003c\/td\u003e\n\u003ctd\u003eTBS2073B\u003c\/td\u003e\n\u003ctd\u003e50 µL of cAMP (1 mM).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS2073C\u003c\/td\u003e\n\u003ctd\u003e110 µL of cAMP-HRP (25x).\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS2073D\u003c\/td\u003e\n\u003ctd\u003e1.1 mL of anti-cAMP antibody (5x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS2073E\u003c\/td\u003e\n\u003ctd\u003e20 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS2073-100--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/12\/TBS2073-cAMP-Colorimetric-Assay_datasheet_v02_2024.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/12\/TBS2073-cAMP-Colorimetric-Assay_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986446409807,"sku":"TBS2073-100","price":2231.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2073.jpg?v=1782811193","url":"https:\/\/www.danyel.co.il\/products\/cyclic-amp-colorimetric-assay-tbs2073-100","provider":"danyel biotech","version":"1.0","type":"link"}