{"product_id":"cyclic-amp-camp-activity-fluorometric-assay-tbs2081-100","title":"Cyclic AMP (cAMP) Activity Fluorometric Assay","description":"\u003cp\u003eThis assay kit is a competition enzyme-linked immunoassay designed to measure cAMP levels in cells or tissues. An anti-mouse IgG was pre-coated onto a microplate. The cAMP in the test sample competes with HRP-cAMP for binding to an anti-cAMP mouse monoclonal antibody. Following the incubation and wash step, an ultra-sensitive HRP fluorescence substrate is added to generate fluorescence can be measured at excitation 535 nm and emission at 585 nm. Because of the competitive nature of this assay, the fluorescent intensity is inversely proportional to the quantity of sample cAMP. Measurement using the cAMP Standard allows calculating the absolute amount of cAMP in a sample. This assay is more sensitive than a colorimetric assay.\u003c\/p\u003e\n\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 2 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of cAMP is typically 1 nM.\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV and the Inter-assay CV are ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes cAMP. No cross-reactivity with others.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStandard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-25144 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2022\/12\/TBS2073-2.png\" alt=\"\" width=\"610\" height=\"333\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStandard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-25155 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/01\/TBS2081-3.png\" alt=\"\" width=\"311\" height=\"266\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"1115\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrecoated Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS2081A\u003c\/td\u003e\n\u003ctd\u003e96 well polystyrene microplate (12 strips of 8 wells) coated with anti-mouse IgG antibody.\u003c\/td\u003e\n\u003ctd\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ecAMP Standard\u003c\/td\u003e\n\u003ctd\u003eTBS2081B\u003c\/td\u003e\n\u003ctd\u003e50 µL of cAMP (1 mM).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS2081C\u003c\/td\u003e\n\u003ctd\u003e100 µL of cAMP-HRP (25x).\u003c\/td\u003e\n\u003ctd\u003eStore at 4℃ for 3 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS2081D\u003c\/td\u003e\n\u003ctd\u003e1 mL of anti-cAMP antibody (5x).\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS2081E\u003c\/td\u003e\n\u003ctd\u003e15 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003ctd\u003eStore at 4℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHRP Fluorescent Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS2081F\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive fluorescent substrate.\u003c\/td\u003e\n\u003ctd\u003eStore at -20℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Developer\u003c\/td\u003e\n\u003ctd\u003eTBS2081G\u003c\/td\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003eStore at 4℃ for 12 months.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS2081-100--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/01\/TBS2081-cAMP-Fluometric-assay-datasheet_V01-2024.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/01\/TBS2081-cAMP-Fluometric-assay_MSDS_2024.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986446999631,"sku":"TBS2081-100","price":2231.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2081-1.jpg?v=1782811213","url":"https:\/\/www.danyel.co.il\/products\/cyclic-amp-camp-activity-fluorometric-assay-tbs2081-100","provider":"danyel biotech","version":"1.0","type":"link"}