{"product_id":"colipase-enzyme-activity-tbp0063","title":"Colipase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 4000-12000 U\/mg solid\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 95%\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eContaminants:\u003c\/u\u003e Free of lipase\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe amount of enzyme causing the release of one micromole of fatty acid from tributyrin per minute at 25°C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003ePancreatic lipase when prepared essentially free of co-lipase is strongly inhibited by conjugated bile salts at or above their critical micell concentration. Addition of co-lipase restores activity to the bile-salt-inhibited lipase and gelfilteration experiments indicate that co-lipase in bile salt solution makes a dimer which forms a 1:1 complex with lipase. Lipase and co-lipase interact as a stoichiometrical relationship and classified co-lipase interact as a co-enzyme for lipase. The co-lipase is a single chain polypeptide with a molecular weight approximately 11,000 with 5 disulfide bridges and isoelectric point 5.0.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eTributyrin (reagent grade).\u003c\/li\u003e\n\u003cli\u003eNaOH, 0.2 M.\u003c\/li\u003e\n\u003cli\u003eLipase (50 Units).\u003c\/li\u003e\n\u003cli\u003eReaction mixture. Dissolve 0.24 g Tris, 2.09 g Sodium tauro-deoxycholate, 0.11 g CaCl2 and 8.76 g NaCl in 800 ml of distilled H2O. Adjust pH to 6.5 with HCl. Bring volume to 1000 ml with distilled H2O.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eInto the reaction vessel of an automatic titrator pipette 15 ml of the reaction mixture and 0.5 ml of Tributyrin at 25°C.\u003c\/li\u003e\n\u003cli\u003eAdd 50 potentiometric units of lipase to the reaction vessel.\u003c\/li\u003e\n\u003cli\u003eMaintaining the pH at 6.5 (and temperature at 22-24°C), titrate the mixture with 0.2 M NaOH over a 5 min period.\u003c\/li\u003e\n\u003cli\u003eCalculate the blank rate.\u003c\/li\u003e\n\u003cli\u003eTo the reaction vessel add 75 μl of a colipase sample that has been diluted 1:10 with distilled H2O.\u003c\/li\u003e\n\u003cli\u003eTitrate the reaction for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eCalculate the rate of reaction. (if the net rate is 0.040-0.095 ml\/min., continue with the calculation. If the rate is outside this range, adjust the concentration of colipase and reanalyze).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBP0063-1MG TBP0063-5MG--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0063-Colipase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0063_Colipase-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 mg","offer_id":45986498543695,"sku":"TBP0063-1MG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 mg","offer_id":45986498576463,"sku":"TBP0063-5MG","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0063.png?v=1782812636","url":"https:\/\/www.danyel.co.il\/products\/colipase-enzyme-activity-tbp0063","provider":"danyel biotech","version":"1.0","type":"link"}