{"product_id":"cho-host-cell-protein-elisa-tbs31004","title":"CHO Host Cell Protein ELISA","description":"\u003cp\u003eTribioscience’s CHO Host Cell Protein ELISA is designed to quantitatively detect CHO HCP levels in samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 1 to 810 ng\/mL. The levels of HCP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for CHO protein.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (R\u003csup\u003e2\u003c\/sup\u003e=0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MDD) of CHO HCP is typically 1 ng\/mL.\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV is ˂ 6.6% and the Inter-assay CV are ˂ 9%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant CHO HCP.\u003c\/p\u003e\n\u003cp\u003eNo cross-reactivity with others.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-9352 size-full\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33004-3.png\" alt=\"\" width=\"617\" height=\"125\" data-lazy-sizes=\"auto, (max-width: 617px) 100vw, 617px\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation \u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-34435 size-full\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/04\/TBS31004-1.png\" alt=\"\" width=\"700\" height=\"369\" data-lazy-sizes=\"auto, (max-width: 700px) 100vw, 700px\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-34436 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/04\/TBS31004_02-1024x810.png\" alt=\"\" width=\"410\" height=\"324\" data-lazy-sizes=\"auto, (max-width: 410px) 100vw, 410px\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"1115\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"60\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"276\"\u003e\u003cstrong\u003eSTORAGE OF OPENED\/ RECONSTITUTED\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003eCHO HCP Microplate\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS31004A\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for CHO HCP.\u003c\/td\u003e\n\u003ctd width=\"276\"\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003eCHO HCP Standard\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS31004B\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e50 µl of Recombinant CHO HCP (8.1 μg\/mL).\u003c\/td\u003e\n\u003ctd width=\"276\"\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS31004C\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e2.1 mL of biotin-CHO HCP antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\" width=\"276\"\u003e \n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003eDetection B\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS31004D\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e200 µL of streptavidin HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS31004E\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e25 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003eWash Buffer\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"102\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"60\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"258\"\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS31004-01--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/04\/TBS31004_CHO-Host-Cell-Protein-ELISA_datasheet.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2021\/04\/TBS31004_CHO-HCP_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986373795919,"sku":"TBS31004","price":3099.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS31004-2.jpg?v=1782809760","url":"https:\/\/www.danyel.co.il\/products\/cho-host-cell-protein-elisa-tbs31004","provider":"danyel biotech","version":"1.0","type":"link"}