{"product_id":"chelating-sepharose-fast-flow-immobilized-metal-affinity-chromatography-resin-17057502","title":"Chelating Sepharose™ Fast Flow immobilized metal affinity chromatography resin","description":"\u003cp\u003eChelating Sepharose Fast Flow is an uncharged IMAC chromatography resin for purification of proteins and peptides with affinity for metal ions. Ready to charge with your metal ion of choice for optimized selectivity.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBased on well-established Sepharose Fast Flow resins.\u003c\/li\u003e\n\u003cli\u003eHigh chemical stability enables proven CIP and sanitization protocols.\u003c\/li\u003e\n\u003cli\u003eHydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool.\u003c\/li\u003e\n\u003cli\u003eFulfills industrial demands for security of supply, robust performance, and regulatory support.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChelating resin for flexible and reliable IMAC purification\u003c\/p\u003e\u003cp\u003eChelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic, which comes immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms. The highly crosslinked agarose resin enables high flow rates and high productivity.\u003c\/p\u003e\u003cp\u003eThis immobilized metal affinity chromatography (IMAC) resin is designed to purify a wide range of proteins. It is supplied free of metal ions (uncharged), allowing the user to charge it with the most appropriate metal ion for purification of a target protein.\u003c\/p\u003e\u003cp\u003eStreamlined scale up\u003c\/p\u003e\u003cp\u003eScale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.\u003c\/p\u003e\u003cp\u003eSuccessful use – research scale\u003c\/p\u003e\u003cp\u003eChelating Sepharose Fast Flow is an established tool for a range of research applications. For instance, this metal chelate affinity chromatography resin has been used to purify his-tagged proteins for the following purposes:\n\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eMap the spatial structure of the \u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/18178548\/\"\u003edimeric transmembrane domain of the growth factor receptor ErbB2\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/9188461\/\"\u003eIdentify distinct PIases\u003c\/a\u003e with proven enzyme activity in \u003ci\u003eE. coli\u003c\/i\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eCompatible empty columns – research scale\u003c\/p\u003e\u003cp\u003eCytiva's solutions include a variety of empty columns for research scale. Chelating Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns.\u003c\/p\u003e\u003cp\u003eCompatible empty columns – bioprocess scale\u003c\/p\u003e\u003cp\u003eFor process-scale applications, Cytiva recommends AxiChrom columns. Chelating Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.\u003c\/p\u003e\u003cp\u003ePrepacked Chelating Sepharose Fast Flow columns – research scale\u003c\/p\u003e\u003cp\u003eThis resin is available as HiTrap columns for use with a syringe, a pump, or a chromatography system.\u003c\/p\u003e\u003cp\u003ePrepacked Chelating Sepharose Fast Flow columns – bioprocess scale\u003c\/p\u003e\u003cp\u003ePrepacked ReadyToProcess columns for bioprocess applications are available on request, eliminating the need for column packing and cleaning validation.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-12767-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-11561-pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"5 L","offer_id":45982337400911,"sku":"17057504","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"50 mL","offer_id":45982337433679,"sku":"17057501","price":2834.0,"currency_code":"ILS","in_stock":true},{"title":"500 mL","offer_id":45982337466447,"sku":"17057502","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-8731-original-png_8926962e-e684-4377-bfb5-8283c609e402.png?v=1785960259","url":"https:\/\/www.danyel.co.il\/products\/chelating-sepharose-fast-flow-immobilized-metal-affinity-chromatography-resin-17057502","provider":"danyel biotech","version":"1.0","type":"link"}