{"product_id":"cgmp-direct-biotrak-eia-rpn226","title":"cGMP Direct Biotrak EIA","description":"\u003cp\u003ecGMP Direct Biotrak EIA.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eElimination of inconvenient, time-consuming extraction procedures.\u003c\/li\u003e\n\u003cli\u003eFlexible method-choice of assay protocols.\u003c\/li\u003e\n\u003cli\u003eDual range 2–512 fmol\/well (acetylation protocol), dual range 50–12 800 fmol\/well (non-acetylation protocol).\u003c\/li\u003e\n\u003cli\u003eRapid assay protocol.\u003c\/li\u003e\n\u003cli\u003eNonradioactive.\u003c\/li\u003e\n\u003cli\u003eSpecific for cGMP\u003c\/li\u003e\n\u003cli\u003ePrecise and accurate measurement.\u003c\/li\u003e\n\u003cli\u003eReady-to-use substrate.\u003c\/li\u003e\n\u003cli\u003eColour coded reagents\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eBiotrak assays\u003c\/p\u003e\u003cp\u003eEIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.\u003c\/p\u003e\u003cp\u003eThe Biotrak cGMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cGMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cGMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.\u003c\/p\u003e\u003cp\u003eLysis reagent 1 hydrolyzes cell membranes to release intra-cellular cAMP. Lysis Reagent 2 sequesters the key component in Lysis Reagent 1 and ensures cAMP is free for subsequent analysis. The detergent\/sequestrant complex does not interfere with antigen: antibody binding. Lysis Reagent 1 is simply added to cultured cells, followed by a 5 to 10 min incubation before assay. The antiserum is reconstituted with Lysis Reagent 2. The assay is based on competition between unlabeled cAMP and a fixed quantity of peroxidase-labeled cAMP, for a limited number of binding sites on a cAMP specific antibody.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/RPN226PL.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"96 wells","offer_id":45982337007695,"sku":"RPN226","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/69c2a45ee6724ce6b5869640310fc044_c0068a0c-cd59-4882-b332-109d471ad662.png?v=1785956229","url":"https:\/\/www.danyel.co.il\/products\/cgmp-direct-biotrak-eia-rpn226","provider":"danyel biotech","version":"1.0","type":"link"}