{"product_id":"cd34-hsc-lnp-kits-29874065","title":"CD34+ HSC LNP kits","description":"\u003cp\u003eThe CD34+ HSC LNP kit is an LNP reagent mix optimized for the delivery of mRNA or Cas9 mRNA\/sgRNA into CD34+ HSCs. This reagent enables researchers to establish a clinically relevant and scalable method at the discovery and preclinical scale for ex vivo gene delivery and editing. CD34+ HSC LNP kit offers the following benefits:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eOptimized LNP formulation: Deliver RNA with high transfection efficiency by using an optimized proprietary LNP formulation*\u003c\/li\u003e\n\u003cli\u003eHigh knockout efficiency: Achieve dose-dependent high efficiency gene knockdown and protein expression.\u003c\/li\u003e\n\u003cli\u003eHigh cell yield and differentiation potential: Maintain high cell viability, yield, and differentiation potential post-editing.\u003c\/li\u003e\n\u003cli\u003eScale from discovery to preclinical: Scale up your ex vivo cell therapy research from discovery to preclinical using the NanoAssemblr™ platform.\u003c\/li\u003e\n\u003cli\u003eValidated workflow: Optimized and validated with the CD34+ HSC cell manufacturing workflow.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e*LNP composition in this research-use-only kit include ionizable lipid excipients that can be licensed for future clinical evaluation.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eEasily integrated into standard CD34+ HSC cell culture protocols\u003c\/p\u003e\n\n\u003cp\u003eThe CD34+ HSC LNP kits can be easily integrated into a CD34+ HSC workflow for both mRNA-based gene expression and CRISPR\/Cas gene editing applications. A general workflow is outlined below.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/TAhrIm8PQCOva0Duhg1BJg-original-png?v=59a646df\" style=\"max-width: 1250px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eSchematic diagram of the LNP treatment and HSC cell culture workflow. LNP production may be decoupled from the cell culture workflow, allowing greater flexibility and simplicity of workflow.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eDose-dependent gene knockout of CD45\u003c\/p\u003e\n\n\u003cp\u003eAchieve dose-dependent highly efficient genome editing of CD34+ HSCs sourced from human cord blood and human mobilized peripheral blood using the CD34+ HSC LNP kit, 100 µL on the NanoAssemblr™ Spark™ system, and CD34+ HSC LNP kit, 2 mL on the NanoAssemblr™ Ignite™ instrument.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/rR_pz-gPR2689T5cqoNVtA-original-png?v=999c0561\" style=\"max-width: 1250px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eA) CD45 dose response of CRISPR-Cas9 mRNA and sgRNA LNPs produced on the NanoAssmblr™ Spark™ instrument. B) Corresponding cell viability. C) Representative images of untreated and CRISPR RNA-LNP treated CFU plates. D) Normalized erythroid and myeloid colony yields of untreated (UT), empty LNP treated, and CRISPR RNA-LNP treated samples.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eCell proliferation and viability following LNP treatment\u003c\/p\u003e\n\n\u003cp\u003eLNP-treated HSCs show high cell proliferation and viability.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/M6ThkxmcRF-I_nf4eRxFgQ-original-png?v=bbd6fe79\" style=\"max-width: 1250px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eA) Cell proliferation and B) viability monitored for over 1 week after LNP-mediated CD45 targeted CRISPR RNA-LNP treated gene editing of HSCs. C) LNP-mediated gene editing compared across n = 2 donors using flow cytometry via CD45 surface expression analysis and D) corresponding histogram.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eHSC cryopreservation and freeze-thawing post-LNP treatment\u003c\/p\u003e\n\n\u003cp\u003eCryopreservation of LNP-treated HSCs showed high cell viability and yield. Furthermore, cells retained their engraftable HSC phenotype distribution while maintaining CD45 knockout efficiency.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/MMoVTqzxSAiQNaM1uJc4mw-original-png?v=e83611fe\" style=\"max-width: 1250px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eCryopreservation of HSCs post-LNP-mediated CRISPR Cas9 gene editing. A) Schematic diagram of the freeze-thaw (F\/T) cycles of HSCs. B) Post-thaw, or non-frozen long-term repopulating HSC phenotype levels, at either 24h or 48h post-LNP treatment freeze time points. C) Cell viability and live cell recovery post 24h-LNP treatment F\/T cycle. D) CD45 knockout efficiency observed in freeze-thawed and fresh LNP-treated HSCs in total live cells and engraftable phenotype (CD34+ CD38- CD90+ CD133+).\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eScale-up of cell culture and RNA-LNP production\u003c\/p\u003e\n\n\u003cp\u003eThe CD34+ HSC LNP kits are available on the NanoAssemblr™ Spark™ and NanoAssemblr™ Ignite™ instruments. Reagents are also available for scaling process development and GMP manufacturing through BioPharma Services.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/YxI-O2j6QoqDTTfxyWbMOQ-original-png?v=1499b484\" style=\"max-width: 1250px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eThe CD34+ HSC LNP kits combined with the NanoAssemblr™ platform enables robust and equivalent performance on both the Spark™ and Ignite™ instruments and offers seamless scalability from discovery to preclinical studies.\u003c\/p\u003e\n\n\u003cp\u003e\u003cimg alt=\"\" class=\"img-responsive center-block\" src=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/d8kdlt2yRkOBImxBNZm1nQ-original-png?v=b1b2f73a\" style=\"max-width: 1250px;\"\u003e\u003c\/p\u003e\n\n\u003cp\u003eA) CD45 knockout and B) CD33 knockout of the total live cell population, as well as C) corresponding cell viability. A-C includes \u0026gt;20 unique experiments with \u0026gt;4 unique donors. D) Untreated, empty-LNP, and CRISPR-Cas9 RNA-loaded LNP treated HSCs were assessed for proliferation using an automated cell counter. E) Untreated, empty-LNP and CRISPR-Cas9 RNA-loaded LNP treated HSCs were assessed for clonogenicity using an automated CFU counter, 5 independent experiments with Spark™ and 2 independent experiments with the Ignite™ instrument.\u003c\/p\u003e\n\n\u003cp class=\"-font--plus-1 -padding--title-md\"\u003eKit components\u003c\/p\u003e\n\n\u003cdiv class=\"table-responsive\"\u003e\n\u003ctable class=\"table table-bordered\"\u003e\n\t\u003cthead\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003cth class=\"-font--body\"\u003e \u003c\/th\u003e\n\t\t\t\u003cth class=\"-font--body\"\u003eCD34+ HSC LNP kit, 100 µL, (Part No. 1003000)\u003c\/th\u003e\n\t\t\t\u003cth class=\"-font--body\"\u003eCD34+ HSC LNP kit, 100 µL with cartridges, (Part No. 1004000)\u003c\/th\u003e\n\t\t\t\u003cth class=\"-font--body\"\u003eCD34+ HSC LNP kit, 2 mL, (Part No. 105000)\u003c\/th\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/thead\u003e\n\t\u003ctbody\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eLipid mix\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e100 µL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e100 µL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e2 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eFormulation buffer type 1\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e400 µL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e400 µL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e1.6 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eDilution buffer type 1\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e1.5 mL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e1.5 mL\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e100 mL (10X)\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eCryopreservation buffer type 2\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e-\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e-\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e6 mL\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eApolipoprotein-E3 (ApoE3)\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e100 µg\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e100 µg\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e500 do\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\t\u003ctr\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003eSpark™ Cartridges\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e-\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e5\u003c\/td\u003e\n\t\t\t\u003ctd class=\"-font--body\"\u003e-\u003c\/td\u003e\n\t\t\u003c\/tr\u003e\n\t\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/01002515_fr.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"Ignite","offer_id":45982289657935,"sku":"1005000","price":806.0,"currency_code":"ILS","in_stock":true},{"title":"Spark (29874065)","offer_id":45982289690703,"sku":"29874065","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Spark (1003000)","offer_id":45982289723471,"sku":"1003000","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/bIJ4A6eTVu4DK6eUe68ag-original-png_2a5b2683-bdbb-4dc1-bea5-252f0caa8b04.png?v=1785959954","url":"https:\/\/www.danyel.co.il\/products\/cd34-hsc-lnp-kits-29874065","provider":"danyel biotech","version":"1.0","type":"link"}