{"product_id":"caspase-family-colorimetric-assay-kit-tbs2050","title":"Caspase-Family Colorimetric Assay Kit","description":"\u003cp\u003eApoptosis plays a fundamental role in many normal biological processes as well as in several disease states. Apoptosis can be induced by various stimuli that all produce the same end result: systematic and orderly cell death.\u003c\/p\u003e\n\u003cp\u003eCaspases cleave a variety of cellular substrates after aspartic acid residues—a characteristic that is central to their role in mammalian apoptosis. Caspases are synthesized in the cytosol of mammalian cells as inactive zymogens, which become active through intracellular caspase cascades.\u003c\/p\u003e\n\u003cp\u003eThe Tribioscience Caspase-Family Colorimetric Assay Kits provides a simple and convenient means for assaying the activity of caspases family members (caspase-1, 2, 3, 4, 5, 6, 8, 9 and 10). The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-family member activity.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKit Components and Storage for 100 Assays\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 45%\" border=\"1px solid black\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eName\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e25 Assays\u003c\/td\u003e\n\u003ctd width=\"73\"\u003eStorage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-1 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-2 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-3 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-4 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-5 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-6 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-8 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-9 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCaspase-10 Substrate\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e30 µL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eCell Lysis Buffer\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e50 mL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eAssay Buffer\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e25 mL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"132\"\u003eDTT (1M)\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e1.8 mL\u003c\/td\u003e\n\u003ctd width=\"73\"\u003e-20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"3\" width=\"278\"\u003eShelf Life: 1year.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eAPPLICATIONS\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eApoptosis\u003c\/li\u003e\n\u003cli\u003eDrug screening\u003c\/li\u003e\n\u003cli\u003eGrowth factors\u003c\/li\u003e\n\u003cli\u003eCytotoxicity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003c!--TBS2050--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS2050-Caspase_Family_Colorimetric_V03-2025.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS2050-Caspase-Family-Colorimetric-Assay-Kit_MSDS_2024-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986443690063,"sku":"TBS2050","price":2683.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2050.png?v=1782811104","url":"https:\/\/www.danyel.co.il\/products\/caspase-family-colorimetric-assay-kit-tbs2050","provider":"danyel biotech","version":"1.0","type":"link"}