{"product_id":"caspase-3-fluorometric-assay-kit-tbs2035","title":"Caspase-3 Fluorometric Assay kit","description":"\u003cp\u003eApoptosis plays a fundamental role in many normal biological processes as well as in several disease states. Apoptosis can be induced by various stimuli that all produce the same result: systematic and orderly cell death.\u003c\/p\u003e\n\u003cp\u003eCaspases cleave a variety of cellular substrates after aspartic acid residues – a characteristic that is central to their role in mammalian apoptosis. Caspases are synthesized in the cytosol of mammalian cells as inactive zymogens, which become active through intracellular caspase cascades.\u003c\/p\u003e\n\u003cp\u003eThe Tribio\u003csup\u003eTM\u003c\/sup\u003e Caspase-3 fluorometric Assay Kits provide a simple and convenient method for the activity analysis of caspases that recognize the sequence DEVD. The assay is based on the detection of the free AFC fluorescence after cleavage from the labeled substrate DEVD-AFC. The free AFC can be quantified using a fluorometer or a fluorescence microtiter plate reader at Ex400\/Em505nm. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows the determination of the fold increase in caspase-3 activity.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eApplications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eApoptosis\u003c\/li\u003e\n\u003cli\u003eDrug screening\u003c\/li\u003e\n\u003cli\u003eGrowth factors\u003c\/li\u003e\n\u003cli\u003eCytotoxicity\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eRelated Products\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/resazurin-cell-viability-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eResazurin Cell Viability (TBS2001)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/ldh-cytotoxicity-assay\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eLDH Cytotoxicity Assay (TBS2002)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/mtt-cell-viability-assay\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMTT Cell Viability Assay (TBS 2003)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/mts-cell-viability-assay\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMTS Cell Viability Assay (TBS2004)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/biochemical-assays\/catalase-assay\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eCatalase Assay (TBS2006)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS2035--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS2035-Caspase3-Fluo_V02-2021.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS2035-Caspase3-Fluo_MSDS_2024-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986427764815,"sku":"TBS2035","price":1317.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2035-1.jpg?v=1782810780","url":"https:\/\/www.danyel.co.il\/products\/caspase-3-fluorometric-assay-kit-tbs2035","provider":"danyel biotech","version":"1.0","type":"link"}