{"product_id":"carboxypeptidase-b-enzyme-activity-tbp0060","title":"Carboxypeptidase B, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 50 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 80%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThat amount of enzyme which hydrolyzes 1 micromole of hippuryl-L-arginine per minute at 25°C and pH 7.65.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eCarboxypeptidase B activity is determined from the increase in absorbance due to the hydrolysis of hippuryl-L-arginine at 254 nm.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eCarboxypeptidase B, (CPB) (EC 3.4.12.3) like carboxypeptidase A, is a pancreatic exopeptidase. Unlike carboxypeptidase A, however, carboxypeptidase B catalyzes the hydrolysis of the peptide bonds involving basic amino acids lysine, arginine and ornithine. This hydrolysis occurs at the C-terminal bond in these polypeptides.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eCarboxypeptidase B shows minimal activity towards Carboxypeptidase A substrates. It is a metalloenzyme containing zinc. Porcine carboxypeptidase B has a molecular weight of 34,300.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e25 mM Tris\/HCl buffer, pH 7.65 (containing 0.1 M NaCl).\u003c\/li\u003e\n\u003cli\u003e1 mM Hippuryl-L-arginine in Tris buffer, pH 7.65.\u003c\/li\u003e\n\u003cli\u003eEnzyme solution: dilute enzyme with double distilled water to a concentration of 1-5 U\/ml.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e(mg\/ml = E278nm x 0.476).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 254 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eInto quartz cuvettes pipette 2.9 ml Hippuryl-L-arginine substrate. Incubate in spectrophotometer for 5 min. to equilibrate and to establish a blank rate, if any.\u003c\/li\u003e\n\u003cli\u003eAdd 0.1 ml. of the enzyme solution to the test cuvette, mix, and record the rate of absorbance at 254 nm for 5 min.\u003c\/li\u003e\n\u003cli\u003eCalculate the (delta)E254nm per minute from the initial linear portion of the curve.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0060-1MG TBP0060-5MG--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0060-Carboxypeptidase-B-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0060_Carboxypeptidase-B-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 mg","offer_id":45986497888335,"sku":"TBP0060-1MG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 mg","offer_id":45986497921103,"sku":"TBP0060-5MG","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0060.png?v=1782812627","url":"https:\/\/www.danyel.co.il\/products\/carboxypeptidase-b-enzyme-activity-tbp0060","provider":"danyel biotech","version":"1.0","type":"link"}