{"product_id":"carbonic-anhydrase-enzyme-activity-tbp0059","title":"Carbonic Anhydrase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 2000 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 10 mg\/ml\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eCarbonic anhydrase (carbonate dehydratase; EC 4.2.1.1) catalyzes the following reaction:\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eThe enzyme is widespread in nature. In animals it plays an important role in respiration by facilitating the transport of carbon dioxide. In plants, carbonic anhydrases are involved in the photosynthetic fixation of CO2.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eMammalian erythrocytes contain two distinct forms of carbonic anhydrase distinguished by differences in their catalytic activities. The enzyme requires zinc for its activity and it has a molecular weight of 30,000.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.05 M Tris-HCl buffer, pH 7.6.\u003c\/li\u003e\n\u003cli\u003e0.003 M p-Nitrophenyl acetate. Dissolve 13.6 mg p-nitrophenyl acetate in 1 ml acetone and dilute with water to a volume of 25 ml. Prepare fresh daily and store in dark.\u003c\/li\u003e\n\u003cli\u003eCarbonic anhydrase (enzyme) solution. Prepare in Tris buffer to yield a final concentration of 1-2 U\/ml. Prepare fresh prior to assay.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet spectrophotometer (equipped with a strip chart recorder and temperature control) at 348 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eIn a cuvette, pipette the following reagents:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003eTris-HCl buffer 1.9 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003ep-nitrophenyl acetate 1.0 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eIncubate in spectrophotometer at 25°C for 5 min.\u003c\/p\u003e\n\u003col start=\"3\"\u003e\n\u003cli\u003eRecord blank rate at 348 nm, if any.\u003c\/li\u003e\n\u003cli\u003eInitiate the reaction by adding 0.1 ml enzyme solution (1-2 U\/ml) to the cuvette. Record the absorbance at 348 nm for 5 min.\u003c\/li\u003e\n\u003cli\u003eCalculate (delta)E348 nm\/min\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0059-1MG TBP0059-5MG--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0059-Carbonic-Anhydrase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0059_Carbonic-Anhydrase-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 mg","offer_id":45986497822799,"sku":"TBP0059-1MG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 mg","offer_id":45986497855567,"sku":"TBP0059-5MG","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0059.png?v=1782812627","url":"https:\/\/www.danyel.co.il\/products\/carbonic-anhydrase-enzyme-activity-tbp0059","provider":"danyel biotech","version":"1.0","type":"link"}