{"product_id":"canine-il-2-fast-elisa-tbs33002","title":"Canine IL-2 Fast ELISA","description":"\u003cp\u003eThe Canine IL-2 Fast ELISA is a solid phase ELISA designed to measure canine IL-2 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 2 hours, not 5-6 hours (Fig. 1). The detection range is from 62 to 4000 pg\/mL. The levels of canine IL-2 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural canine IL-2 protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eTypical Data\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (R\u003csup\u003e2\u003c\/sup\u003e=1) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MDD) of canine IL-2 is typically 62 pg\/mL.\u003cbr\u003e\nThe Intra-CV is \u0026lt; 10%, the Inter-CV is \u0026lt; 10%.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eSpecificity\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant canine IL-2.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-9352 size-full lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33004-3.png\" alt=\"\" width=\"617\" height=\"125\" data-lazy-sizes=\"auto, (max-width: 617px) 100vw, 617px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-31835 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33002_01.png\" alt=\"\" width=\"704\" height=\"410\" data-lazy-sizes=\"auto, (max-width: 704px) 100vw, 704px\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-31836 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33002_02.png\" alt=\"\" width=\"465\" height=\"398\" data-lazy-sizes=\"auto, (max-width: 465px) 100vw, 465px\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"height: 350px\" border=\"1px solid black\" width=\"1115\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003e\u003cstrong\u003ePART\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"66\"\u003e\u003cstrong\u003ePART#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e\u003cstrong\u003eDESCRIPTION\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd width=\"274\"\u003e\u003cstrong\u003eSTORAGE OF OPENED\/ RECONSTITUTED\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003eCanine IL-2 Microplate\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS33002A\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for Canine IL-2.\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eThe unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003eCanine IL-2 Standard\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS33002B\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e30 μL of Recombinant Canine IL-2 protein (200 ng\/mL).\u003c\/td\u003e\n\u003ctd width=\"274\"\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003eDetection A\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS33002C\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e220 µL of Biotinylated Canine IL-2 antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\" width=\"274\"\u003e \n\u003cp\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003eDetection B\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS33002D\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e300 µL of Streptavidin-HRP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS33002E\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e15 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000W\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e15 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000T\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"96\"\u003eStop Solution\u003c\/td\u003e\n\u003ctd width=\"66\"\u003eTBS3000S\u003c\/td\u003e\n\u003ctd width=\"247\"\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS33002--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33002_canine_IL-2_elisa.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33002_Canine_IL-2_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986389622863,"sku":"TBS33002","price":1869.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS33002.jpg?v=1782810453","url":"https:\/\/www.danyel.co.il\/products\/canine-il-2-fast-elisa-tbs33002","provider":"danyel biotech","version":"1.0","type":"link"}