{"product_id":"bst-dna-polymerase-exonuclease-minus-pcramp-001","title":"Bst DNA Polymerase, Exonuclease Minus","description":"\u003cp\u003eBst DNA Polymerase, Exonuclease Minus, is a recombinant form of the 67 kDa Bacillus stearothermophilus DNA Polymerase protein (large fragment). The enzyme has 5’-3’ polymerase activity and strand displacement activity, but it lacks 3’-5’ exonuclease activity. It also has reverse transcription activity.\u003c\/p\u003e\u003cp\u003eBst DNA Polymerase, Exonuclease Minus, has higher strand displacement activity than that of other suppliers (Figure 1). The enzyme can be used in nucleic acid amplification methods* such as isothermal amplification, whole genome amplification (WGA), and multiple displacement amplification (MDA). It also can be used in Next Generation sequencing.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eStrand displacement amplification\u003cbr\u003eDNA sequencing through high GC regions (1,2)\u003cbr\u003eRapid sequencing from nanogram amounts of DNA template (3)\u003c\/p\u003e\u003cp\u003eThis enzyme has optimal activity at 65°C. It is suitable for sequencing DNA with high GC content and secondary structures. It is available in concentrations of 8,000 U\/ml or 50,000 U\/ml.\u003c\/p\u003e\u003cp\u003eBst DNA Polymerase, Exonuclease Minus, is supplied with 10X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH 4) 2SO 4, 100 mM KCl, 20 mM MgSO 4, and 1.0 % Triton X-100.\u003c\/p\u003e\u003cp\u003eFigure 1. Bst DNA Polymerase, Exonuclease Minus, possesses greater strand-displacing polymerase activity. M13 single stranded DNA was incubated with or without 8 units of Bst DNA Polymerase(+\/- Bst) in reaction buffer supplied by the manufacturer, with or without replication primer (+\/- primer) for 30 minutes at 65°C. MW, 1 kb ladder.\u003c\/p\u003e\u003cp\u003eTips for use:\u003c\/p\u003e\u003cp\u003eRequires 0.1% Triton X-100 for long term storage.\u003cbr\u003eReaction temperatures above 70°C are not recommended.\u003cbr\u003eBst DNA Polymerase cannot be used for thermal cycle sequencing.\u003c\/p\u003e\u003cp\u003eHeat Inactivation: 80ºC for 20 min.\u003c\/p\u003e\u003cp\u003eReferences:\u003c\/p\u003e\u003cp\u003eGriffin, H. and Griffin, A. (1994) PCR Technology, 228-229.\u003cbr\u003eMcClary, J. et al. (1991) J. DNA Sequencing and Mapping, 1, 173-180.\u003cbr\u003eMead, D.A. et al. (1991) Biotechniques, 11, 76-87.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh strand displacement activity.\u003c\/li\u003e\n\u003cli\u003eUsed in isothermal amplification and Next Generation Sequencing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eage Buffer:\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eBst DNA Polymerase, Exonuclease Minus is available in two concentrations, 8,000 U\/ml and 50,000 U\/ml, in a storage buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, and 50% Glycerol. Also supplied is 10X DNA Polymerase Buffer B, composed of 200 mM Tris-HCl pH 8.8, 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4,  and 1.0 % Triton X-100.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-001_30027-Bst-DNA-Polymerase-Exonuclease-Minus\"\u003e30027 Bst DNA Polymerase Exonuclease Minus\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-001_30028-1-Bst-DNA-Polymerase-Exonuclease-Minus-50000U-per-mL\"\u003e30028 1 Bst DNA Polymerase Exonuclease Minus 50000U per mL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-001_SDS-30027-Bst-DNA-Polymerase-Exonuclease-Minus\"\u003eSDS 30027 Bst DNA Polymerase Exonuclease Minus\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_PCRAMP-001_SDS-30028-1-Bst-DNA-Polymerase-Exonuclease-Minus-50000U-per-mL\"\u003eSDS 30028 1 Bst DNA Polymerase Exonuclease Minus 50000U per mL\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-001_Bst-DNA-Polymerase-50-U\"\u003eBst DNA Polymerase 50 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_PCRAMP-001_Bst-DNA-Polymerase-8-U\"\u003eBst DNA Polymerase 8 U\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_PCRAMP-001_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Exonuclease Minus 8,000 U\/mL, 2,000 U","offer_id":45986429141071,"sku":"30027-1","price":660.0,"currency_code":"ILS","in_stock":true},{"title":"Exonuclease Minus Kit, 40,000 U at 8,000 U\/mL","offer_id":45986429173839,"sku":"30027-3","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Exonuclease Minus 50,000 U\/mL, 10,000 U","offer_id":45986429206607,"sku":"30028-1","price":2616.0,"currency_code":"ILS","in_stock":true},{"title":"Exonuclease Minus,  8,000 U\/mL, 40,000 U","offer_id":45986429239375,"sku":"F93635-2","price":9293.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Bst-DNA-Polymerase_252C-Exonuclease-Minus_fb12678e-04a1-4b90-bd76-50e88d8673b0.jpg?v=1785956068","url":"https:\/\/www.danyel.co.il\/products\/bst-dna-polymerase-exonuclease-minus-pcramp-001","provider":"danyel biotech","version":"1.0","type":"link"}