{"product_id":"botulinum-neurotoxin-type-a-bont-a-elisa-tbs31007","title":"Botulinum Neurotoxin Type A (BoNT-A) ELISA","description":"\u003cp\u003eTribioscience’s Botulinum Neurotoxin Type A (BoNT-A) ELISA is designed to quantitatively detect Botulinum Neurotoxin Type A (BoNT-A) levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 46.9 to 3000 pg\/mL. The levels of Botulinum Neurotoxin Type A (BoNT-A) samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural Botulinum Neurotoxin Type A (BoNT-A) protein.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eTypical Data\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis standard curve (R\u003csup\u003e2\u003c\/sup\u003e=0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe minimum detectable dose (MOD) of BoNT-A is typically 46 pg\/mL.\u003c\/p\u003e\n\u003cp\u003eThe Intra-assay CV and the Inter-assay CV are ˂10%.\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSpecificity\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis assay recognizes natural and recombinant BoNT-A.\u003c\/p\u003e\n\u003cp\u003eNo cross-reactivity with others.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Procedures (Fig. 1)\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-9352 size-full lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2020\/04\/TBS33004-3.png\" alt=\"\" width=\"617\" height=\"125\" data-lazy-sizes=\"(max-width: 617px) 100vw, 617px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eSimple Standard Preparation \u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-14383 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/06\/TBS31007-2.png\" alt=\"\" width=\"651\" height=\"353\" data-lazy-sizes=\"auto, (max-width: 651px) 100vw, 651px\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eAccurate Standard Curve\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-14427 \" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/06\/TBS31007-3.png\" alt=\"\" width=\"354\" height=\"274\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eKit Content and Storage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"1115\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePart\u003c\/th\u003e\n\u003cth\u003ePart#\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003cth\u003eStorage of Opened\/Reconstituted\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eBotulinum Neurotoxin Type A Microplate\u003c\/td\u003e\n\u003ctd\u003eTBS31007A\u003c\/td\u003e\n\u003ctd\u003e96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for BoNT-A.\u003c\/td\u003e\n\u003ctd\u003eReturn unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBotulinum Neurotoxin Type A Standard\u003c\/td\u003e\n\u003ctd\u003eTBS31007B\u003c\/td\u003e\n\u003ctd\u003e50 µl of Recombinant Botulinum Neurotoxin Type A (30 ng\/mL).\u003c\/td\u003e\n\u003ctd\u003eAliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection A\u003c\/td\u003e\n\u003ctd\u003eTBS31007C\u003c\/td\u003e\n\u003ctd\u003e2.1 mL of biotin-Botulinum Neurotoxin Type A antibody.\u003c\/td\u003e\n\u003ctd rowspan=\"6\"\u003eMay be stored for up to 3 months at 2-8 °C.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDetection B\u003c\/td\u003e\n\u003ctd\u003eTBS31007D\u003c\/td\u003e\n\u003ctd\u003e200 µL of streptavidin HRP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Diluent\u003c\/td\u003e\n\u003ctd\u003eTBS31007E\u003c\/td\u003e\n\u003ctd\u003e25 mL of a buffered protein base with preservatives.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10x Wash Buffer\u003c\/td\u003e\n\u003ctd\u003eTBS3000W\u003c\/td\u003e\n\u003ctd\u003e12 mL of concentrated solution (10x).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTMB Substrate\u003c\/td\u003e\n\u003ctd\u003eTBS3000T\u003c\/td\u003e\n\u003ctd\u003e12 mL of ultra-sensitive TMB substrate.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003eTBS3000S\u003c\/td\u003e\n\u003ctd\u003e6 mL of 2 N sulfuric acid.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003eStore the unopened kit at 2-8 ℃. Do not use past kit expiration dates.\u003cbr\u003e\nThe kit contains sufficient materials to run an ELISA on one 96-well plate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Product\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/fast-human-phospho-tau-thr217-pt217-elisa\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman p-Tau-217 ELISA (TBS3293)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/fast-human-phospho-tau-thr181-p-t181-elisa\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman p-Tau-181 ELISA (TBS3294)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/fast-human-mapt-tau-total-elisa\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman Total Tau ELISA (TBS3295)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/fast-human-phospho-tau-thr231-p-t231-elisa\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman p-Tau-231 ELISA (TBS3296)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/elisa\/fast-human-ad7c-ntp-elisa\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eHuman AD7c NTP (TBS3297)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS31007--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/06\/TBS31007-Botulinum-Neurotoxin-Type-A-BoNT-A-Elisa-datasheet-6-2023-3.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/06\/TBS31007_Botulinum-Neurotoxin_ELISA_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReferences\u003c\/h2\u003e\n\u003cp\u003eHudson, C., Wilson, P., Lieberman, D., Mittelman, H., \u0026amp; Parikh, S. (2024). Analysis of Breast Milk Samples in Lactating Women After Undergoing Botulinum Toxin Injections for Facial Rejuvenation: A Pilot Study. \u003ci\u003eFacial plastic surgery \u0026amp; aesthetic medicine\u003c\/i\u003e, 10.1089\/fpsam.2023.0326. Advance online publication. \u003ca href=\"https:\/\/doi.org\/10.1089\/fpsam.2023.0326\" target=\"_blank\" rel=\"noopener\"\u003ehttps:\/\/doi.org\/10.1089\/fpsam.2023.0326\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986373926991,"sku":"TBS31007","price":2643.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS31007.jpg?v=1782809765","url":"https:\/\/www.danyel.co.il\/products\/botulinum-neurotoxin-type-a-bont-a-elisa-tbs31007","provider":"danyel biotech","version":"1.0","type":"link"}