{"product_id":"beta-nicotimamide-adenine-dinucleotide-reduced-nadh-tbp0098","title":"beta-Nicotimamide-Adenine Dinucleotide, Reduced, NADH","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Crystalline powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular Weight:\u003c\/u\u003e 709.4\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003ePurity:\u003c\/u\u003e 98+%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eNADH is used in the determination of aldolase, glutamate dehydrogenase, transaminases, a-hydroxybutyrate dehydrogenase, lipase, malate dehydrogenase, sorbitol dehydrogenase, nucleoside monophosphate, nucleoside diphosphate, nucleoside triphosphate, ammonia, carbon dioxide, creatinine, free fatty acuds, uric acid urea nitrogen, pyruvate, triglycerides and other enzymes and metabolites.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.1M Triethanolamine buffer\/substrate, pH 7.6: 1.86 g TEA\u0026amp;HCl, 210 mg glycerate-3-P, 125 mg MgSO4\u0026amp;7H2O and 50 mg EDTA with 80 ml distilled water. Adjust pH to 7.6 with 1M NaOH-Na2 and adjust volume to 100 ml with distilled water.\u003c\/li\u003e\n\u003cli\u003e18.2 mM Sodium Pyruvate: 0.20 mg pyruvate-Na in 10 ml distilled water.\u003c\/li\u003e\n\u003cli\u003eLDH, from rabbit muscle (5 mg protein\/ml): 500 U\/mg.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDissolve 25 mg NADH in 25 ml distilled water in a volumetric flask.\u003c\/li\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eInto a cuvette, pipette the following:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003eBuffer                         (1)     3.50 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eSodium pyruvate        (2)     0.50 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eMix and read the absorbance A1.\u003c\/p\u003e\n\u003col start=\"4\"\u003e\n\u003cli\u003eAdd 0.10 ml of the sample. Mix and read absorbance A2.\u003c\/li\u003e\n\u003cli\u003eStart the reaction by adding 0.01 ml LDH. Mix and read absorbance A3.\u003c\/li\u003e\n\u003cli\u003eAdd an additional 0.01 ml LDH. Mix and read absorbance A4.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0098-1G TBP0098-5G--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0098-beta-Nicotimamide-Adenine-Dinucleotide-Reduced.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0098_beta-Nicotimamide-Adenine-Dinucleotide-Reduced-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 g","offer_id":45986502180943,"sku":"TBP0098-1G","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 g","offer_id":45986502213711,"sku":"TBP0098-5G","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0098-1.png?v=1782812707","url":"https:\/\/www.danyel.co.il\/products\/beta-nicotimamide-adenine-dinucleotide-reduced-nadh-tbp0098","provider":"danyel biotech","version":"1.0","type":"link"}