{"product_id":"beta-nicotimamide-adenine-dinucleotide-phosphate-reduced-nadph-tbp0097","title":"beta-Nicotimamide-Adenine Dinucleotide Phosphate, Reduced, NADPH","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Crystalline powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular Weight:\u003c\/u\u003e 833.4\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003ePurity:\u003c\/u\u003e 98+%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eNADPH is used in the determination of leucine aminopeptidase, creatine, ammonia and urea.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.1M Triethanolamine buffer\/substrate, pH 7.6: 1.86 g TEA\u0026amp;HCl, 210 mg glycerate-3-P, 125 mg MgSO4\u0026amp;7H2O and 50 mg EDTA with 80 ml distilled water. Adjust pH to 7.6 with 1M NaOH-Na2 and adjust volume to 100 ml with distilled water.\u003c\/li\u003e\n\u003cli\u003e3 M Ammoniumcholride:.16.5 g NH4Cl in distilled water, adjust volume to 100 ml.\u003c\/li\u003e\n\u003cli\u003e0.2 M a-Ketoglutarate: 45 mg a-Ketoglutarate-Na2\u0026amp;2H2O in 1 ml distilled water.\u003c\/li\u003e\n\u003cli\u003eGlutamate dehydrogenase, from bovine liver (20 mg protein\/ml): 120 U\/mg.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDissolve 25 mg NADP in 25 ml distilled water in a volumetric flask.\u003c\/li\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eInto a cuvette, pipette the following:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003eBuffer           (1)     2.50 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eNH4Cl          (2)     0.15 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003ea-KG             (3)     0.10 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eMix and read the absorbance A1.\u003c\/p\u003e\n\u003col start=\"4\"\u003e\n\u003cli\u003eAdd 0.10 ml of the sample. Mix and read absorbance A2.\u003c\/li\u003e\n\u003cli\u003eStart reaction by adding 0.01 ml GLDH. Mix and read absorbance A3.\u003c\/li\u003e\n\u003cli\u003eAdd an additional 0.01 ml GLDH. Mix and read absorbance A4 (absorbance due to enzyme).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0097-100MG TBP0097-500MG--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0097-beta-Nicotimamide-Adenine-Dinucleotide-Phosphate-Reduced.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0097_beta-Nicotimamide-Adenine-Dinucleotide-Phosphate-Reduced-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"100 mg","offer_id":45986502115407,"sku":"TBP0097-100MG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"500 mg","offer_id":45986502148175,"sku":"TBP0097-500MG","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0097-1.png?v=1782812707","url":"https:\/\/www.danyel.co.il\/products\/beta-nicotimamide-adenine-dinucleotide-phosphate-reduced-nadph-tbp0097","provider":"danyel biotech","version":"1.0","type":"link"}