{"product_id":"beta-nicotimamide-adenine-dinucleotide-phosphate-nadp-tbp0096","title":"beta-Nicotimamide Adenine Dinucleotide Phosphate, NADP","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Crystalline powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular Weight:\u003c\/u\u003e 823.4\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003ePurity:\u003c\/u\u003e 98+%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eNADP is used in the determination of amylase, creatine kinase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and glucose.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.1M Triethanolamine buffer\/substrate, pH 7.6: 1.86 g TEA¿HCl, 210 mg glycerate-3-P, 125 mg MgSO4¿7H 2O and 50 mg EDTA with 80 ml distilled water. Adjust pH to 7.6 with 1M NaOH-Na 2 and adjust volume to 100 ml with distilled water.\u003c\/li\u003e\n\u003cli\u003e0.1 M MgCl2: 2.03 g MgCl2¿6H 2O in 100 ml distilled water.\u003c\/li\u003e\n\u003cli\u003e14 mM D,L-Isocitrate: 4 mg D,L-isocitrate-Na3 in 1 ml TEA buffer.\u003c\/li\u003e\n\u003cli\u003eIsocitrate dehydrogenase, from porcine heart (5 mg protein\/ml): 4 U\/mg\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDissolve 50 mg NADP in 50 ml distilled water in a volumetric flask.\u003c\/li\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eInto a cuvette, pipette the following: sample 0.10 ml Mix and read the absorbance A1.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003eBuffer                         (1)     2.50 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eMgCl 2                        (2)     0.10 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eD,L-isocitrate             (3)     0.10 ml\u003c\/p\u003e\n\u003col start=\"4\"\u003e\n\u003cli\u003eAdd 0.02 ml of the ICDH. Mix and read absorbance A2.\u003c\/li\u003e\n\u003cli\u003eAdd an additional 0.02 ml ICDH. Mix and read absorbance A 3 (absorbance due to enzyme).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0096-1G TBP0096-5G--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0096-beta-Nicotimamide-Adenine-Dinucleotide-Phosphate.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0096_beta-Nicotimamide-Adenine-Dinucleotide-Phosphate-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 g","offer_id":45986501853263,"sku":"TBP0096-1G","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 g","offer_id":45986501886031,"sku":"TBP0096-5G","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0096-1.png?v=1782812703","url":"https:\/\/www.danyel.co.il\/products\/beta-nicotimamide-adenine-dinucleotide-phosphate-nadp-tbp0096","provider":"danyel biotech","version":"1.0","type":"link"}