{"product_id":"beta-nicotimamide-adenine-dinucleotide-nad-tbp0095","title":"beta-Nicotimamide Adenine Dinucleotide, NAD","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Crystalline powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular Weight:\u003c\/u\u003e 663.4\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003ePurity:\u003c\/u\u003e \u0026gt;95%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eNAD is used in the determination of amylase, creatine kinase, transaminases, lactate dehydrogenase, phosphohexose isomerase, ethanol, galactose, glucose, uric acid, L-lactate, triglycerides and other enzymes and metabolites.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSodiumdiphophate\/semicarbazide\/substrate, pH 8.7: 3.33 g Na4P2O7\u0026amp;10 H2O, 0.84 g semicarbazide\u0026amp;HCl, 0.17 glycine, 1.00 ml ethanol with 80 ml distilled water. Adjust pH to 8.7 with 2 M NaOH; adjust volume to 100 ml.\u003c\/li\u003e\n\u003cli\u003eAlcohol dehydrogenase, from yeast (30 mg protein\/ml): 300 U\/ml.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDissolve 25 mg NAD in 25 ml distilled water in a volumetric flask.\u003c\/li\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eInto a cuvette, pipette the following:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003eBuffer  (1)     3.00 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003esample          0.10 ml\u003c\/p\u003e\n\u003col start=\"4\"\u003e\n\u003cli\u003eMix and read the absorbance A1\u003c\/li\u003e\n\u003cli\u003eStart the reaction by adding 0.01 ml of Alcohol dehydrogenase (2). Mix and read absorbance A2.\u003c\/li\u003e\n\u003cli\u003eAdd another 0.01 ml Alcohol dehydrogenase. Mix and read absorbance A3.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0095-1G TBP0095-5G--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0095-beta-Nicotimamide-Adenine-Dinucleotide.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0095_beta-Nicotimamide-Adenine-Dinucleotide-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 g","offer_id":45986501754959,"sku":"TBP0095-1G","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 g","offer_id":45986501787727,"sku":"TBP0095-5G","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0095-1.png?v=1782812701","url":"https:\/\/www.danyel.co.il\/products\/beta-nicotimamide-adenine-dinucleotide-nad-tbp0095","provider":"danyel biotech","version":"1.0","type":"link"}