{"product_id":"beta-glucuronidase-enzyme-activity-tbp0058","title":"beta-Glucuronidase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe amount of enzyme which catalyzes the formation of one micromole of p-Nitrophenol per minute at 37°C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eß-Glucuronidase (EC 3.2.1.31) catalyzes the following reaction:\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eIt has been found in tissue extracts of mammals and other vertebrates, digestive juices of snails, molluscs, locusts, bacteria and plants. The enzyme catalyzes the hydrolysis of §-glucuronides and also the transfer of glucuronly radicals to acceptor alcohols. §-Glucuronidase is a structural protein of the endoplasmic reticulum and its occurance in the lysozomes is due to changes in the endoplasmic reticulum.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.1 M Acetate buffer, pH 4.5.\u003c\/li\u003e\n\u003cli\u003e0.1M p-Nitrophenyl glucuronide.\u003c\/li\u003e\n\u003cli\u003eEnzyme solution in distilled water.\u003c\/li\u003e\n\u003cli\u003e0.5 N NaOH.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet up spectrophotometer at 405 nm and 37°C.\u003c\/li\u003e\n\u003cli\u003ePlace the following reagents in a test tube and equilibrate in the water bath at 37°C for about 15 minutes:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003e0.1 M Acetate buffer, pH 4.5    1.0  ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003ep-Nitrophenyl glucuronide       0.03 ml\u003c\/p\u003e\n\u003col start=\"3\"\u003e\n\u003cli\u003eAdd 0.03 ml of the enzyme solution and mix.\u003c\/li\u003e\n\u003cli\u003eAfter the test tubes have incubated for exactly 10 minutes at 37°C, add 2.0 ml of 0.5 N NaOH solution to stop the reaction (ODtest). At the same time, prepare the blank by first mixing the reagent mixture with 2.0 ml of NaOH solution after the 10 minute incubation period at 37¡C, and then adding the enzyme solution (ODblank).\u003c\/li\u003e\n\u003cli\u003eMeasure the absorbance of the test (ODtest) and blank (ODblank) at 405nm against H2O.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0058-500KU TBP0058-1000KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0058-beta-Glucuronidase.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0058_beta-Glucuronidase-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"500 KU","offer_id":45986497658959,"sku":"TBP0058-500KU","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1000 KU","offer_id":45986497691727,"sku":"TBP0058-1000KU","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0058-1.png?v=1782812623","url":"https:\/\/www.danyel.co.il\/products\/beta-glucuronidase-enzyme-activity-tbp0058","provider":"danyel biotech","version":"1.0","type":"link"}