{"product_id":"baseline-zero-dnase-db0715k","title":"Baseline-ZERO DNase","description":"\u003cp\u003eBaseline-ZERO™ DNase* digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I. Even the small DNA oligonucleotides that remain after treatment with bovine pancreatic DNase I are undetectable by gel electrophoresis following treatment with Baseline-ZERO DNase (Figure. 2). Removal of DNA from RNA preparations is particularly beneficial when RNA in a sample is amplified using a method that involves reverse transcription using random primers, since any contaminating DNA would also be a template for random-primed cDNA synthesis.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eRemoval of genomic DNA from small-sample total RNA preparations for expression analysis (Figure. 1)\u003cbr\u003eRemoval of small DNA oligonucleotides (e.g. random primers)\u003c\/p\u003e\u003cp\u003eFigure 1. Real-time PCR of HeLa RNA preparations treated with various DNases. The lower the C T value (intersection of curves with the red line), the greater the amount of residual DNA not digested by the indicated DNase. Thus, Baseline-ZERO™ DNase removed all detectable DNA from the RNA sample. The TaqMan® probe assay amplified a 268-bp fragment of β-actin. Samples were run in duplicate.\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eKienzle, N. et al. (1996) BioTechniques 20, 612.\u003c\/p\u003e\u003cp\u003e*Patent pending.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEffectively digest large or small dsDNA and ssDNA into mononucleotides\u003c\/li\u003e\n\u003cli\u003eDigest unwanted dsDNA and ssDNA molecules including small ssDNA such as random hexamers\u003c\/li\u003e\n\u003cli\u003eUse in sensitive applications requiring reverse transcription where any contaminating DNA is unwanted\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eUnit Definition: One Molecular Biology Unit (MBU) of Baseline-ZERO™ DNase produces an increase in the A260 of a solution of dsDNA, of 0.001 per minute at 25 °C. Functionally, 1 MBU completely digests 1 µg of linear pUC19 DNA to mononucleotides in 10 minutes at 37 °C.\u003c\/p\u003e\u003cp\u003eStorage Buffer: Baseline-ZERO DNase is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 0.1% Triton® X-100.\u003c\/p\u003e\u003cp\u003e10X Baseline-ZERO™ DNase Reaction Buffer: 100 mM Tris HCl (pH 7.5), 25 mM MgCl2 and 5 mM CaCl2.\u003c\/p\u003e\u003cp\u003e10X Baseline-ZERO™ DNase Stop Solution: 30 mM EDTA.\u003c\/p\u003e\u003cp\u003eQuality Control: Baseline-ZERO DNase is assayed for its ability to completely digest linear dsDNA to mononucleotides under standard assay conditions. Baseline-ZERO DNase is free of detectable RNase activities as assayed by PAGE analysis of 1 µg of a synthetic RNA transcript following an overnight incubation with sufficient DNase I to completely digest 1,000 µg of DNA.\u003c\/p\u003e\u003cp\u003eread more\u003c\/p\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eIncludes a 10X reaction buffer and a 10X stop solution.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_DB0715K_Baseline-ZERO-DNase\"\u003eBaseline ZERO DNase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_DB0715K_baseline-zero-dnase\"\u003ebaseline zero dnase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/pis_DB0715K_Enzyme-Catalog-Full\"\u003eEnzyme Catalog Full\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"Default Title","offer_id":45986440118351,"sku":"DB0715K","price":2050.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Baseline-ZERO-DNase_0ef00e42-5cbe-40ad-bd75-b15a236892fa.jpg?v=1785956219","url":"https:\/\/www.danyel.co.il\/products\/baseline-zero-dnase-db0715k","provider":"danyel biotech","version":"1.0","type":"link"}