{"product_id":"apoperoxidase-enzyme-activity-tbp0057","title":"Apoperoxidase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e None\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 95%\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eRZ Value:\u003c\/u\u003e \u0026lt;0.1\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe amount of enzyme causing the production of one milligram of purpurgallin in 20 seconds at 25°C under the given assay conditions.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe rate of decomposition of hydrogen peroxide catalyzed by peroxidase, with 4-aminoantipyrine as hydrogen donor, is determined by measuring the increase in absorbance at 510 nm.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003ePeroxidase (EC 1.11.1.7) is an enzyme catalyzing the oxidation, by hydrogen peroxide, of a number of substrates such as ascorbate, cytochrome C and the leuco form of many dyes.\u003c\/p\u003e\n\u003cp\u003eHorseradish peroxidase (HRP) exists in the form of several isozymes, all containing heme as the prosthetic group. The enzyme has a molecular weight of approximately 40,000.\u003c\/p\u003e\n\u003cp\u003eThe ability of peroxidase to catalyze the oxidation of a number of organic compounds by hydrogen peroxide, resulting in formation of colored end-products, is utilized in several methods of determination of glucose and galactose in biological fluids. Also, recently a method has been described for the determination of plasma uric acid utilizing uricase and horseradish peroxidase.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.2 M Potassium phosphate, pH 7.0.\u003c\/li\u003e\n\u003cli\u003e0.0017 M Hydrogen peroxide. Prepare by diluting 1 ml of 30% H2O2 to 100 ml with distilled water. Further dilute 1 ml of this solution to 50 ml using 0.2 M phosphate buffer, pH 7.0. Prepare fresh daily.\u003c\/li\u003e\n\u003cli\u003e0.0025 M 4-Aminoantipyrine with 0.17 M phenol. Dissolve 810 mg phenol in 40 ml distilled water. Add 25 mg 4-aminoantipyrine and made the volume up to 50 ml with distilled water.\u003c\/li\u003e\n\u003cli\u003eEnzyme (peroxidase) solution. Dissolve 1 mg\/ml in distilled water. Just prior to use, dilute further with distilled water to yield a concentration of 0.05-0.25 U\/ml. Set spectrophotometer (equipped with strip chart recorder and temperature control) at 510 nm and 25°C.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 510 nm and 25°C.\u003c\/li\u003e\n\u003cli\u003eInto the cuvette, pipette the following:\u003c\/li\u003e\n\u003cli\u003ePhenol\/4-aminoantipyrine solution 1.4 ml\u003c\/li\u003e\n\u003cli\u003eHydrogen peroxide solution 1.5 ml\u003c\/li\u003e\n\u003cli\u003eMix the cuvette contents and incubate in spectrophotometer at 25°C for 3-4 minutes to achieve temperature equilibrium.\u003c\/li\u003e\n\u003cli\u003eEstablish blank rate, if any, at 510 nm.\u003c\/li\u003e\n\u003cli\u003eInto the cuvette, pipette 0.1 ml enzyme solution (0.05-0.25 U\/ml). Mix and record the increase in absorbance at 510 nm for 4-5 minutes.\u003c\/li\u003e\n\u003cli\u003eCalculate µE510 nm\/min\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0057-1KU TBP0057-5KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0057-Apoperoxidase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0057_Apoperoxidase-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1000 U","offer_id":45986497593423,"sku":"TBP0057-1000 U","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5000 U","offer_id":45986497626191,"sku":"TBP0057-5000U","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0057.png?v=1782812623","url":"https:\/\/www.danyel.co.il\/products\/apoperoxidase-enzyme-activity-tbp0057","provider":"danyel biotech","version":"1.0","type":"link"}