{"product_id":"ampligase-thermostable-dna-ligase-a0110","title":"Ampligase Thermostable DNA Ligase","description":"\u003cp\u003eAmpligase™ Thermostable DNA Ligase catalyses NAD-dependent ligation of adjacent 3´-hydroxylated and 5´-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. Its half-life is 48 hours at 65°C and greater than 1 hour at 95°C. Ampligase DNA Ligase has been shown to be active for at least 500 thermal cycles (94°C\/80°C) or 16 hours of cycling. 10 This exceptional thermostability permits extremely high hybridization stringency and ligation specificity. Ampligase DNA Ligase has no detectable activity in ligating blunt-ended DNA and has no activity on RNA or RNA:DNA hybrids.\u003c\/p\u003e\u003cp\u003eApplications\u003c\/p\u003e\u003cp\u003eLigation amplification (Ligase Chain Reaction, LCR) 1-7\u003cbr\u003eRepeat Expansion Detection (RED) 7\u003cbr\u003eSimultaneous mutagenesis of multiple sites 8,9\u003cbr\u003eOther ligation-based detection methods\u003c\/p\u003e\u003cp\u003eReferences\u003c\/p\u003e\u003cp\u003eLandegren, U. et al. (1988) Science 242, 229.\u003cbr\u003eWu, D.Y. and Wallace, R.B. (1989) Genomics 4, 560.\u003cbr\u003eBarany, F. (1991) Proc. Natl. Acad. Sci. USA 88, 189.\u003cbr\u003eBirkenmeyer, L. and Armstrong, A.S. (1992) J. Clin. Micro. 30, 3089.\u003cbr\u003eDille, B.J. et al. (1993) J. Clin. Micro. 31, 729\u003cbr\u003eNakazawa, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 360.\u003cbr\u003eSirugo, G. and Kidd, K. (1995) Epicentre Forum 2(3), 1.\u003cbr\u003eMoore, D. and Michael, S. (1995) Epicentre Forum 2(4), 4.\u003cbr\u003eRouwendal, G.J. et al. (1993) BioTechniques 15, 68.\u003cbr\u003eSchalling, M. et al. (1993) Nature Genetics 4, 135.\u003cbr\u003eHardenbol, P. et al. (2003) Nature Biotechnology 21, 673.\u003c\/p\u003e\n\u003ch2\u003eKey features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHigh thermostability allows ligation using high-stringency hybridization conditions\u003c\/li\u003e\n\u003cli\u003eHigh specificity and stringency permits sensitive detection of SNPs\u003c\/li\u003e\n\u003cli\u003eAvailable in kit and \"enzyme only\" formats11\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProduct Details\u003c\/h2\u003e\n\u003cp\u003eAmpligase Thermostable DNA Ligase starter Kit (Item ID A8101) includes Ampligase Thermostable DNA Ligase, 10X Ampligase Reaction Buffer, and a DNA ligation control. All other Ampligase Thermostable DNA Ligase Kits include Ampligase DNA Ligase and 10X Ampligase Reaction Buffer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/sds_A0110_A0102K-A32750-A3202K-Ampligase-Enzyme-and-Buffer\"\u003eA0102K A32750 A3202K Ampligase Enzyme and Buffer\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/SDS_A0110_A0110K-A3210K-Ampligase-DNA-Ligase\"\u003eA0110K A3210K Ampligase DNA Ligase\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/biosearchtech.a.bigcontent.io\/v1\/static\/manual_A1905B_MA023E-Ampligase-Thermostable-DNA-Ligase\"\u003eMA023E Ampligase Thermostable DNA Ligase\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Biosearch Technologies","offers":[{"title":"100 U\/µL, 2,500 units","offer_id":45986434416719,"sku":"A0102K","price":1977.0,"currency_code":"ILS","in_stock":true},{"title":"100 U\/uL, 10,000 U","offer_id":45986434449487,"sku":"A0110K","price":5085.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 100,000 U","offer_id":45986434482255,"sku":"A3100","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"2,500 U @ 5 U\/µL","offer_id":45986434515023,"sku":"A3202K","price":2080.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 10,000 U","offer_id":45986434547791,"sku":"A3210K","price":5176.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 750 U","offer_id":45986434580559,"sku":"A32750","price":965.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/µL, 1,000 U","offer_id":45986434613327,"sku":"A8101","price":1350.0,"currency_code":"ILS","in_stock":true},{"title":"5 U\/uL, 20 mL, 100,000 U","offer_id":45986434646095,"sku":"E0001-5D5","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/Ampligase-Thermostable-DNA-Ligase_d0f154b6-ab31-4a17-b536-ca6f3ef116c9.jpg?v=1785956168","url":"https:\/\/www.danyel.co.il\/products\/ampligase-thermostable-dna-ligase-a0110","provider":"danyel biotech","version":"1.0","type":"link"}