{"product_id":"amersham-shrimp-alkaline-phosphatase-e70092y","title":"Amersham Shrimp Alkaline Phosphatase","description":"\u003cp\u003eAlkaline phosphatase is suitable for dephosphorylation of 5' and 3'-overhangs, recessed ends, blunt ends, and single nucleotides. Following incubation, alkaline phosphatase can be easily inactivated by heating.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBacterial alkaline phosphatase recombinantly expressed in E. coli.\u003c\/li\u003e\n\u003cli\u003eActive in PCR and restriction endonuclease buffers.\u003c\/li\u003e\n\u003cli\u003eInactivated by heating at 75°C for 5 minutes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eUse together with \u003ca href=\"\/shop\/molecular-biology\/pcr-and-amplification\/gel-extraction-and-clean-up\/illustra-exonuclease-i-p-03102\"\u003eExonuclease\u003c\/a\u003e I for cleaning up PCR reactions prior to downstream application such as DNA sequencing.\u003c\/p\u003e\n\n\u003cp\u003eUnit Definition\u003c\/p\u003e\n\n\u003cp\u003eOne unit is the amount of the enzyme required to dephosphorylate 1 µg of linearized pUC57 DNA termini in 10 minutes at 37°C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29046320.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"500 units","offer_id":45982218649679,"sku":"E70092Y","price":690.0,"currency_code":"ILS","in_stock":true},{"title":"1000 units","offer_id":45982218682447,"sku":"E70092Z","price":1109.0,"currency_code":"ILS","in_stock":true},{"title":"5000 units","offer_id":45982218715215,"sku":"E70092X","price":3722.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-34534-original-png_938e7402-0ecd-43e3-b0d5-ccded476da4f.png?v=1785959145","url":"https:\/\/www.danyel.co.il\/products\/amersham-shrimp-alkaline-phosphatase-e70092y","provider":"danyel biotech","version":"1.0","type":"link"}