{"product_id":"amersham-microspin-g-50-columns-27533002","title":"Amersham MicroSpin G-50 Columns","description":"\u003cp\u003eMicroSpin G-50 columns are designed for the rapid purification of DNA in desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor rapid purification of labeled DNA (DNA probes of \u0026gt; 20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μL using spin-column chromatography.\u003c\/li\u003e\n\u003cli\u003eColumns are prepacked with Sephadex G-50 DNA Grade pre-equilibrated in TE buffer with 0.05% Kathon CG\/ICP Biocide.\u003c\/li\u003e\n\u003cli\u003eProduct recovery will be at least 10% lower than that from ProbeQuant G-50 Micro Columns.\u003c\/li\u003e\n\u003cli\u003eCan also be used for buffer exchange\/desalting of enzymatic reactions (10 to 100 μl) following heat denaturation and phenol extraction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eMicroSpin G-50 Columns, rapid purification of DNA requiring clean-up\u003c\/p\u003e\n\n\u003cp\u003eGood product yield and purity is obtained with sample volumes from 12-50 μL. They are suitable for any DNA greater than 20 bases in length and will not remove or denature enzymes.\u003c\/p\u003e\n\n\u003cp\u003eThe resin used in these columns, Sephadex G-50 DNA Grade, can be used for a wide range of applications, including desalting DNA, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.\u003c\/p\u003e\n\n\u003cp\u003eSephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is a well-established size exclusion resin for desalting and buffer exchange of biomolecules \u0026gt; 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.\u003c\/p\u003e\n\n\u003cp\u003eAlternatively, Sephadex G-25 or Sephadex G-100 can be used. Sephadex G-25 has an exclusion limit of approximately Mr 5000, and with a spin protocol this means it can be used for any DNA greater than 10 bases in length.\u003c\/p\u003e\n\n\u003cp\u003eSephadex G-100 DNA Grade has an exclusion limit of 25bp ds DNA.\u003c\/p\u003e\n\n\u003cp\u003eAll these Sephadex types are therefore suitable for the purification of oligonucleotides or small DNA fragments following synthesis or a labeling reaction.\u003c\/p\u003e\n\n\u003cp\u003e\u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-25-dna-grade-p-00054\"\u003eSephadex G-25 DNA Grade\u003c\/a\u003e, \u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-50-dna-grade-p-00113\"\u003eSephadex G-50 DNA Grade\u003c\/a\u003e, and \u003ca href=\"\/shop\/molecular-biology\/purification\/gel-filtration-columns\/illustra-sephadex-g-100-dna-grade-p-00198\"\u003eSephadex G-100\u003c\/a\u003e are all sold separately.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/27533001PL.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"50 columns","offer_id":45982270062671,"sku":"27533001","price":1447.0,"currency_code":"ILS","in_stock":true},{"title":"250 columns","offer_id":45982270095439,"sku":"27533002","price":5822.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-6180-original-png_647b77f2-3326-45ee-bf3c-c3131dfbc4f0.png?v=1785959658","url":"https:\/\/www.danyel.co.il\/products\/amersham-microspin-g-50-columns-27533002","provider":"danyel biotech","version":"1.0","type":"link"}