{"product_id":"amersham-hot-start-master-mix-25150001","title":"Amersham Hot Start Master Mix","description":"\u003cp\u003eHot Start Master Mix is a 2X premixed formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRecombinant hot start activator protein sequesters primers prior to PCR, effectively reducing nonspecific priming and primer-dimer formation.\u003c\/li\u003e\n\u003cli\u003eQuick hot start activation preserves sample integrity by avoiding extensive precycling heat treatment.\u003c\/li\u003e\n\u003cli\u003eAvoids the risk of contamination from a mammalian source associated with hot start antibodies.\u003c\/li\u003e\n\u003cli\u003eConvenient, ready-to-use mix saves time and reduces experimental variability.\u003c\/li\u003e\n\u003cli\u003eConfidence in downstream applications; increased read length in sequencing applications.\u003c\/li\u003e\n\u003cli\u003eFor any standard PCR reaction, such as multiplex PCR, and reactions exhibiting nonspecific amplification or primer-dimer formation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eDescription\u003c\/p\u003e\n\n\u003cp\u003eHot Start Master Mix uses a novel hot start method called primer sequestration.\u003c\/p\u003e\n\n\u003cp\u003eHot Start Master Mix combines high-quality recombinant \u003ci\u003eTaq\u003c\/i\u003e DNA Polymerase, a recombinant hot start activator protein, and nucleotides in a proprietary reaction buffer. This ready-to-use mix provides robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success. The mix does not contain \u003ci\u003eTaq\u003c\/i\u003e antibody and this eliminates the risk of contamination from a mammalian source. Also, since the polymerase is not chemically inactivated, there is no extensive precycling heating step necessary; this reduces the chance of damaging precious DNA samples from heat-induced depurination. The net result from using Hot Start Master Mix is a more robust and reliable amplification of the target amplicons with increased yield when compared to conventional hot start master mix products.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/01iMeiuj40CUDBAIANLasw-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29474447.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"100 reactions","offer_id":45982270292047,"sku":"25150001","price":972.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-10111-original-png_722f95d6-9837-4963-ac9e-993de243b0e8.png?v=1785959665","url":"https:\/\/www.danyel.co.il\/products\/amersham-hot-start-master-mix-25150001","provider":"danyel biotech","version":"1.0","type":"link"}