{"product_id":"amersham-ecl-western-blotting-detection-reagent-rpn2106","title":"Amersham™ ECL Western Blotting Detection Reagent","description":"\u003cp\u003eEnhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eIdeal for confirmatory Western blotting applications using chemiluminescence detection\u003c\/li\u003e\n\u003cli\u003eAt least 10-fold more sensitive than colorimetric assays\u003c\/li\u003e\n\u003cli\u003eDetectable on  Amersham Hyperfilm ECL or with a CCD imager such as ImageQuant LAS 500.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eDiscover our \u003cstrong\u003e\u003ca href=\"\/education\/online-learning\/object?id=course:1802641?extcmp=cy21389-gl-gcr-gen-western-blotting-elearning-rd-wbelearning\"\u003eWestern Blotting: tips and troubleshooting eLearning course\u003c\/a\u003e\u003c\/strong\u003e. Learn approaches to ensure high-quality western blotting courses and receive a certificate upon course completion.\u003c\/p\u003e\n\n\u003cp\u003eHigh sensitivity chemiluminescence detection on Western blotting\u003c\/p\u003e\n\n\u003cp\u003eAmersham ECL Western Blotting Detection Reagents use enhanced luminol-based detection, which is suitable for all routine Western blotting. ECL Western Blotting Detection Reagents, in combination with Amersham Protran membranes, form a complete solution for Western blot applications.\u003c\/p\u003e\n\n\u003cp\u003e\u003cbr\u003e\nReliable chemiluminescence detection\u003c\/p\u003e\n\n\u003cp\u003eIn most contemporary ECL systems a luminol peroxide detection reagent is added to the membrane and reacts with the horseradish peroxidase enzyme (HRP) conjugated to the secondary antibody. HRP catalyzes the oxidation of luminol in a multistep reaction and is accompanied by the emission of low-intensity light at 428 nm, which can be measured with light-sensitive X-ray film or with a CCD imager.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/0RPN2106.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"For 1 000cm² membrane","offer_id":45982270619727,"sku":"RPN2109","price":784.0,"currency_code":"ILS","in_stock":true},{"title":"For 2 000cm² membrane","offer_id":45982270652495,"sku":"RPN2209","price":1243.0,"currency_code":"ILS","in_stock":true},{"title":"For 4 000cm² membrane","offer_id":45982270685263,"sku":"RPN2106","price":1729.0,"currency_code":"ILS","in_stock":true},{"title":"For 6 000cm² membrane","offer_id":45982270718031,"sku":"RPN2134","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-7056-original-png_d70f83e5-e9c6-4ff5-937d-668f3cedfa6e.png?v=1785959673","url":"https:\/\/www.danyel.co.il\/products\/amersham-ecl-western-blotting-detection-reagent-rpn2106","provider":"danyel biotech","version":"1.0","type":"link"}