{"product_id":"amersham-ecl-western-blotting-detection-kit-rpn2108","title":"Amersham™ ECL Western Blotting Detection Kit","description":"\u003cp\u003eEach kit contains the detection reagent, HRP conjugated antibodies, and blocking agent sufficient for detection of 1000 cm² membrane.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDetection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.\u003c\/li\u003e\n\u003cli\u003eOptimized system provides rapid, sensitive results using simple protocols.\u003c\/li\u003e\n\u003cli\u003eDesigned for use with nitrocellulose and PVDF membranes.\u003c\/li\u003e\n\u003cli\u003eHigh sensitivity: Up to 10 times more sensitive than colorimetric methods.\u003c\/li\u003e\n\u003cli\u003eRapid: Results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.\u003c\/li\u003e\n\u003cli\u003eEasy reprobing: Save time and materials by reprobing on membranes up to 10 times.\u003c\/li\u003e\n\u003cli\u003eQuantifiable: Linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,\u003c\/li\u003e\n\u003cli\u003eNon-radioactive-no specialized facilities needed\u003c\/li\u003e\n\u003cli\u003eProven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAmersham ECL Western Blotting Detection Kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.\u003c\/p\u003e\u003cp\u003eECL Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system.\u003c\/p\u003e\u003cp\u003eEach system contains the following reagents, sufficient for detection of 1000 cm² membrane: \nAnti-mouse HRP conjugate, 100 µl\nAnti-rabbit HRP conjugate, 100 µl\nECL Detection Reagents 1 and 2, 62.5 ml each\nBlocking agent, 5 g.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/0RPN2106.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"For detecting 1000 cm²","offer_id":45982271176783,"sku":"RPN2108","price":1193.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-33746-original-png_961b3025-2647-4d05-ba34-25a312bc114f.png?v=1785959687","url":"https:\/\/www.danyel.co.il\/products\/amersham-ecl-western-blotting-detection-kit-rpn2108","provider":"danyel biotech","version":"1.0","type":"link"}