{"product_id":"adenosyl-homocysteinase-ahcy-fluorometric-assay-tbs2056","title":"Adenosyl homocysteinase (AHCY) Fluorometric Assay","description":"\u003cp\u003eAdenosylhomocysteinase (AHCY) or S-adenosylhomocysteine hydrolase (SAHH) is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine (Hcy). In mammals, AHCY is the only enzyme capable of performing this reaction. SAH is the by-product and a potent inhibitor of methyltransferase activity. AHCY deletion is embryonic lethal in many organisms (from plants to mammals). In humans, AHCY deficiency is associated with an incurable rare recessive disorder in methionine metabolism.\u003c\/p\u003e\n\u003cp\u003eTribioscience’s AHCY Activity Assay utilizes SAH as the substrate to form homocysteine and adenosine. The free thiol group of the homocysteine can be detected with a thiol probe generating an enhanced fluorescent signal at Ex\/Em = 400\/465 nm. The assay principle is displayed in Figure 1. The kit provides the easiest and most accurate approach to measuring AHCY activity in a variety of samples.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eFig. 1: Principle of AHCY Assay\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-19439 lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/08\/TBS2056-2.png\" alt=\"\" width=\"442\" height=\"86\" data-lazy-sizes=\"auto, (max-width: 442px) 100vw, 442px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMeasure AHCY activity in a variety of samples.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStorage Conditions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eStore the Reagent at –20° (–80°C for AHCY Enzyme positive control), protected from light.\u003c\/p\u003e\n\u003cp\u003eShelf life: 6 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eStandard Curve \u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg class=\"alignnone wp-image-24345 size-full lazyloaded\" src=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/08\/TBS2056-2-1.png\" alt=\"\" width=\"422\" height=\"303\" data-lazy-sizes=\"auto, (max-width: 422px) 100vw, 422px\" data-was-processed=\"true\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS2056--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/08\/TBS2056_AHCY_Activity-Fluorometric-Assay-datasheet_V02_2024.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2023\/08\/TBS2056_Adenosyl-homocysteinase-Fluorometric-Assay_MSDS.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986443788367,"sku":"TBS2056","price":3142.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS2056-1.png?v=1782811108","url":"https:\/\/www.danyel.co.il\/products\/adenosyl-homocysteinase-ahcy-fluorometric-assay-tbs2056","provider":"danyel biotech","version":"1.0","type":"link"}