{"product_id":"adenosine-deaminase-enzyme-activity-tbp0052","title":"Adenosine Deaminase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 3 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 95%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003e\u003cspan style=\"font-size: 18px\"\u003eUnit\u003c\/span\u003e Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eOne unit will deaminate 1.0 \u0026amp;mgr;mole of adenosine to inosine per min at pH 7.4 at 25° C.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 17px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.05 M Phosphate buffer, pH 7.4.\u003c\/li\u003e\n\u003cli\u003eAdenosine, 3.6 mg\/ml dissolved in buffer.\u003c\/li\u003e\n\u003cli\u003eEnzyme solution in buffer (1-2 U\/ml).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet up spectrophotometer (with strip chart and temp. control) to 265 nm and 25° C.\u003c\/li\u003e\n\u003cli\u003eInto a cuvette, pipette the following:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003ePhosphate buffer 2.97 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eAdenosine            0.01 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eIncubate cuvette in spectrophotometer at 25° C for 5 minutes to attain temperature equillibration and then establish blank rate if any.\u003c\/p\u003e\n\u003col start=\"3\"\u003e\n\u003cli\u003eInitiate reaction by adding 0.02 ml enzyme solution.\u003c\/li\u003e\n\u003cli\u003eCalculate E265nm\/min\u003c\/li\u003e\n\u003c\/ol\u003e\n\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAdenosine Deaminase (EC 3.5.4.4) is widely distributed in animal tissues. The highest enzyme activities are found in the mucosa of the small intestine, in the appendix and the spleen. The enzyme is present in the cytoplasmic cell fraction and also to some extent in the nucleus. The optimum pH is 7.0 to 7.4.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBP0052-250U TBP0052-1KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0052-Adenosine-Deaminase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0052_Adenosine-Deaminase-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"250 U","offer_id":45986497003599,"sku":"TBP0052-250U","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 KU","offer_id":45986497036367,"sku":"TBP0052-1KU","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0052.png?v=1782812607","url":"https:\/\/www.danyel.co.il\/products\/adenosine-deaminase-enzyme-activity-tbp0052","provider":"danyel biotech","version":"1.0","type":"link"}