{"product_id":"adenosine-5-monophosphate-amp-tbp0093","title":"Adenosine 5′-monophosphate, AMP","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Crystalline powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular Weight:\u003c\/u\u003e 391.19\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eTriethanolamine buffer, 0.1M, pH 7.6: 1.86 g TEA• HCl in 80 ml distilled water. Adjust to pH 7.6 with 1.0 M NaOH, adjust volume to 100 ml with distilled water.\u003c\/li\u003e\n\u003cli\u003e14mM NADH:10 mg NADH-Na2 with 1 ml distilled water.\u003c\/li\u003e\n\u003cli\u003e16.5 mM ATP: 10 mg NADH-Na2 in 1 ml distilled water.\u003c\/li\u003e\n\u003cli\u003e0.5 M MgSO4\/2 M KCl: 1.23 g MgSO4• 7 H2O and 1.49 g KCl in 10 ml distilled water.\u003c\/li\u003e\n\u003cli\u003e32 mM Phosphoenolpyruvate: 15 mg PEP-(CHA)3 in 1 ml MgSO4\/KCl.\u003c\/li\u003e\n\u003cli\u003eLDH, from rabbit muscle: 5 mg protein\/ml (550 U\/mg).\u003c\/li\u003e\n\u003cli\u003ePyruvate kinase, from rabbit muscle: 10 mg protein\/ml (200 U\/mg).\u003c\/li\u003e\n\u003cli\u003eMyokinase, from rabbit muscle: 5 mg protein\/ml (360 U\/mg).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAdenosine 5′-monophosphate (AMP) is used in the determination of creatine kinase and 5′-nucleotidase.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDissolve 50 mg AMP in 50 ml distilled water in a volumetric flask.\u003c\/li\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25° C.\u003c\/li\u003e\n\u003cli\u003eInto two cuvettes, pipette the following:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 200px\"\u003eBLANK SAMPLE\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eBuffer           (1)          3.00 ml  3.00 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eNADH          (2)          0.05 ml  0.05 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eATP              (3)          0.01 ml  0.01 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003ePEP               (5)          0.15 ml  0.15 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eLDH              (6)          0.01 ml  0.10 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003ePK                (7)          0.02 ml  0.02 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eDistilled H2O              0.05 ml  – – –\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eSample                        – – –        0.05 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eMix and read the absorbance A\u003csub\u003e1\u003c\/sub\u003e.\u003c\/p\u003e\n\u003col start=\"4\"\u003e\n\u003cli\u003eStart the reaction by adding 0.01 ml myokinase (8) to both the blank and sample. Read the absorbance A2.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0093-5G TBP0093-25G--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0093-Adenosine-5-monophosphate.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0093_Adenosine-5-monophosphate-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"5 g","offer_id":45986501591119,"sku":"TBP0093-5G","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"25 g","offer_id":45986501623887,"sku":"TBP0093-25G","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0093-1.png?v=1782812697","url":"https:\/\/www.danyel.co.il\/products\/adenosine-5-monophosphate-amp-tbp0093","provider":"danyel biotech","version":"1.0","type":"link"}