{"product_id":"adenosine-5-diphosphate-adp-tbp0092","title":"Adenosine 5′-diphosphate, ADP","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Crystalline powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular Weight:\u003c\/u\u003e 507.20\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe assay is based on the reactions described by J.L. Strominger, L.A. Heppel, E.S. Maxwell, Biochim.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAdenosine 5′-diphosphate (ADP) is used in the determination of creatine kinase, glutamate dehydrogenase, urea and ammonia.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eTriethanolamine buffer, 0.1M, pH 7.6: 1.86 g TEA• HCl in 80 ml distilled water. Adjust to pH 7.6 with 1.0 M NaOH, adjust volume to 100 ml with distilled water.\u003c\/li\u003e\n\u003cli\u003e14mM NADH:10 mg NADH-Na2 with 1 ml distilled water.\u003c\/li\u003e\n\u003cli\u003e0.5 M MgSO4\/2 M KCl: 1.23 g MgSO4• 7H2O and 1.49 g KCl in 10 ml distilled water.\u003c\/li\u003e\n\u003cli\u003e32 mM PEP: 5 mg PEP-(CHA)3 in 1 ml solution (3).\u003c\/li\u003e\n\u003cli\u003eLDH, from rabbit muscle: 5 mg protein\/ml (~550 U\/mg).\u003c\/li\u003e\n\u003cli\u003ePK, from rabbit muscle: 10 mg protein\/ml (200 U\/mg).\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eDissolve 50 mg ADP in 50 ml distilled water in a volumetric flask.\u003c\/li\u003e\n\u003cli\u003eSet spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25° C.\u003c\/li\u003e\n\u003cli\u003eInto a cuvette, pipette the following:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"padding-left: 40px\"\u003eBuffer           (1)                        2.70 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eNADH          (2)                        0.05 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003ePEP               (4)                        0.15 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003eLDH              (5)                        0.02 ml\u003c\/p\u003e\n\u003cp style=\"padding-left: 40px\"\u003esample                                       0.10 ml\u003c\/p\u003e\n\u003col start=\"4\"\u003e\n\u003cli\u003eMix and read the absorbance A\u003c\/li\u003e\n\u003cli\u003eStart the reaction by adding 0.02 ml PK (6) and read the absorbance A2.\u003c\/li\u003e\n\u003cli\u003eWhen the reaction is complete add an additional 0.02 ml PK (6). Mix and read the absorbance A3.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0092-1G TBP0092-5G--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0092-Adenosine-5-diphosphate.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0092_Adenosine-5-diphosphate-Safety-Data-Sheet-1.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 g","offer_id":45986501460047,"sku":"TBP0092-1G","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5 g","offer_id":45986501492815,"sku":"TBP0092-5G","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0092-1.png?v=1782812694","url":"https:\/\/www.danyel.co.il\/products\/adenosine-5-diphosphate-adp-tbp0092","provider":"danyel biotech","version":"1.0","type":"link"}