{"product_id":"acylase-1-enzyme-activity-tbp0051","title":"Acylase 1, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder, salt free\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Store at -20° C (-4° F)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 1000 U\/mg protein\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 90%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThat amount of enzyme which catalyzes the hydrolysis of one micromole of N-Acetyl-L-Methionine per hour at 25° C, pH 7.0.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe assay is based on the reaction described by Mitz and Schulueter, Biochim. The enzyme catalysis is followed by measuring absorbance at 238 nm.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAcylase 1 (EC 3.5.1.14) is an extremely stable enzyme in the dry state. In solution, the enzyme is stable at high temperatures (70° C) at pH 7, but below pH 5 it is rapidly and irreversibly inactivated. The enzyme shows a high degree of optical specificity toward its substrates. For this reason, it has been used extensively for the resolution of racemic amino acids.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.1 M Potassium phosphate buffer, pH 7.0.\u003c\/li\u003e\n\u003cli\u003e0.015 M N-Acetyl-L-Methionine (2.87 mg\/ml). Dissolve in 0.01 M potassium phosphate buffer. Adjust pH to 7.0 with 2 M NaOH if necessary.\u003c\/li\u003e\n\u003cli\u003eAcylase 1 (enzyme) solution. Dilute in 0.01 M potassium phosphate buffer, pH 7.0 to yield a concentration of 1000-2000 U\/ml. Prepare fresh prior to assay.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet the spectrophotometer (equipped with strip chart recorder and temperature control) at 238 nm and 25° C.\u003c\/li\u003e\n\u003cli\u003eIn a cuvette place 2.9 ml of 0.015 M N-Acetyl-L-Methionine (substrate). Incubate cuvette in spectrophotometer at 25°C for 5 minutes.\u003c\/li\u003e\n\u003cli\u003eRecord absorbance at 238 nm (blank).\u003c\/li\u003e\n\u003cli\u003eInitiate the reaction by adding 0.1 ml enzyme solution to the cuvette. Follow the reaction by measuring the absorbance at 238 nm for 5-8 minutes.\u003c\/li\u003e\n\u003cli\u003eCalculate E238nm\/min\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0051-500MG TBP0051-1G--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0051-Acylase-1-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0051_Acylase-1-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"500 mg","offer_id":45986496741455,"sku":"TBP0051-500MG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 g","offer_id":45986496774223,"sku":"TBP0051-1G","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0051.png?v=1782812604","url":"https:\/\/www.danyel.co.il\/products\/acylase-1-enzyme-activity-tbp0051","provider":"danyel biotech","version":"1.0","type":"link"}