{"product_id":"acid-phosphatase-enzyme-activity-tbp0050","title":"Acid Phosphatase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProduct Details\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eForm:\u003c\/u\u003e Freeze-dried powder\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eSolubility:\u003c\/u\u003e Soluble in distilled water or dilute buffer\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Stable when stored at -20°C\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e 10-20 U\/mg solid\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eProtein:\u003c\/u\u003e 95%\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eUnit Definition\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe amount of enzyme which liberates one micromole p-nitrophenyl per minute at 37°C and pH 4.8.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eAssay Method\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThe rate of the reaction is determined by measuring the increase in absorbance at 405 nm resulting from the release of p-nitrophenol from p-nitrophenyl phosphate.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eAcid phosphatase(EC 3.1.3.2) is widely distributed in nature in plants, animals and microorganisms. Potatoes, wheat germ, milk and bovine prostate gland are generally used as sources for commercial quantities of this enzyme. One of the most concentrated sources of acid phosphatase is the human prostate gland. Clinically, serum acid phosphatase levels are used for diagnosis of prostatic cancer.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eReagents\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e0.1 M NaOH.\u003c\/li\u003e\n\u003cli\u003eBuffer\/Substrate Solution (0.05 M sodium citrate buffer, pH 4.8, with 5.5 mM p-nitrophenyl phosphate).\u003c\/li\u003e\n\u003cli\u003eAcid phosphatase (enzyme) solution. Dissolve in 0.05 M sodium citrate buffer, pH 4.8 to prepare a concentration of 1 mg\/ml. Dilute as required. Must be prepared fresh prior to assay.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProcedure\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eSet the water bath at 37°C.\u003c\/li\u003e\n\u003cli\u003eInto a two test tubes pipette the following reagents in the amounts indicated: TEST BLANK\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eBuffer\/Substrate solution                                          1.0 ml 1.0 ml\u003c\/p\u003e\n\u003cp\u003eDiluted enzyme                                                       0.2 ml ———\u003c\/p\u003e\n\u003cp\u003e0.1 M NaOH                                                            ——— 1.0 ml\u003c\/p\u003e\n\u003col start=\"3\"\u003e\n\u003cli\u003eMix and incubate test tube in water bath for 30 minutes at 37°C.\u003c\/li\u003e\n\u003cli\u003eAdd the following to each test tube and mix:\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e0.1 M NaOH                                                            1.0 ml ———\u003c\/p\u003e\n\u003cp\u003eDiluted enzyme                                                        ——— 0.2 ml\u003c\/p\u003e\n\u003col start=\"5\"\u003e\n\u003cli\u003eMeasure increase in absorbance at 405 nm using spectrophotometer against blank.\u003c\/li\u003e\n\u003cli\u003eCalculate the ΔE405nm\/min\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBP0050-500MG TBP0050-1G--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0050-Acid-Phosphatase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/03\/TBP0050_Acid-Phosphatase-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"500 mg","offer_id":45986497200207,"sku":"TBP0050-500MG","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"1 g","offer_id":45986497232975,"sku":"TBP0050-1G","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0050.png?v=1782812614","url":"https:\/\/www.danyel.co.il\/products\/acid-phosphatase-enzyme-activity-tbp0050","provider":"danyel biotech","version":"1.0","type":"link"}