{"product_id":"aavpro-helper-free-system-aav1-cre-recombinase-6668","title":"AAVpro® Helper Free System (AAV1-CRE Recombinase)","description":"\u003cp\u003eThis system is used to prepare AAV1 particles that will deliver a Cre recombinase gene cassette to mammalian cells. Cre recombinase is a bacterial protein that recognizes a specific 34-bp sequence (loxP site) containing an 8-bp core sequence where recombination takes place. The orientation and location of introduced loxP sites allow for Cre-mediated gene inversion, translocation, or deletion.  With this kit, high-titer AAV1 particles can be prepared without a helper virus by simply transfecting HEK 293T cells with the three included plasmids that encode all of the factors necessary to prepare recombinant AAV1 particles.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHelper virus-free preparation of high-titer AAV particles encoding the Cre recombinase gene under the control of the CMV promoter\u003c\/li\u003e\n\u003cli\u003eEnables delivery of the Cre recombinase gene to hard-to-transfect mammalian cells, including proliferating and non-proliferating cells\u003c\/li\u003e\n\u003cli\u003eCre recombinase mediates recombination between loxP sites, allowing gene inversion, translocation, or deletion\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eGenome editing of target cells containing a floxed DNA sequence\u003cbr\u003eTransgenic mouse applications\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD4807\/SD4807-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pRC2-mi342.gb\"\u003epRC-mi342 vector sequence in .gb format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/6236_pAAV-CRE%20Recombinase.fasta\"\u003epAAV-Cre Recombinase vector sequence in .fasta format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/6230_pRC2-mi342.fasta\"\u003epRC2-mi342 vector sequence in .fasta format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pHelper.gb\"\u003epHelper vector sequence in .gb format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/6653_v201511w_y99990101_COA.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/6654_v201511w_y99990101_COA.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/6668_v201511w_y99990101_COA.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 ug each","offer_id":45986011086927,"sku":"6668","price":3938.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3310_lr.jpg?v=1782801713","url":"https:\/\/www.danyel.co.il\/products\/aavpro-helper-free-system-aav1-cre-recombinase-6668","provider":"danyel biotech","version":"1.0","type":"link"}