{"product_id":"a-glucosidase-enzyme-activity-tbp0049","title":"a-Glucosidase, Enzyme Activity","description":"\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003ePreparation and Specification\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eAppearance:\u003c\/u\u003e White amorphous powder, lyophilized\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eActivity:\u003c\/u\u003e GradeⅡ　20U\/mg-solid or more\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eContaminants:\u003c\/u\u003e α-amylase ≤1.0×10\u003csup\u003e-5\u003c\/sup\u003e%\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStabilizers:\u003c\/u\u003e BSA\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eProperties\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eStability:\u003c\/u\u003e Stable at −20℃ for at least one year\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMolecular weight:\u003c\/u\u003e approx. 65,000 (Gel-filtration and SDS-PAGE)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eIsoelectric point\u003c\/u\u003e: 5.2\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eMichaelis constant:\u003c\/u\u003e 6.3×10\u003csup\u003e-4\u003c\/sup\u003eM (p-Nitrophenyl-α-D-glucopyranoside)\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eInhibitors:\u003c\/u\u003e Ag\u003csup\u003e＋\u003c\/sup\u003e, Hg\u003csup\u003e＋＋\u003c\/sup\u003e, PCMB, MIA\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eOptimum pH:\u003c\/u\u003e 6.0−7.0\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eOptimum temperature:\u003c\/u\u003e 60℃\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003epH Stability:\u003c\/u\u003e pH 5.0−9.0\u003c\/p\u003e\n\u003cp\u003e\u003cu\u003eThermal stability:\u003c\/u\u003e below 60℃ (pH 7.0, 15min)\u003c\/p\u003e\n\u003ctable border=\"1px solid black\" width=\"660\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\"\u003eSubstrate*\u003c\/td\u003e\n\u003ctd width=\"162\"\u003eRelative hydrolysis rate**\u003c\/td\u003e\n\u003ctd width=\"162\"\u003eSubstrate*\u003c\/td\u003e\n\u003ctd width=\"174\"\u003eRelative hydrolysis rate**\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\"\u003ePNPG\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e100.0\u003c\/td\u003e\n\u003ctd width=\"162\"\u003eMaltose\u003c\/td\u003e\n\u003ctd width=\"174\"\u003e271.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\"\u003ePNPG2\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e205.0\u003c\/td\u003e\n\u003ctd width=\"162\"\u003eMaltotriose\u003c\/td\u003e\n\u003ctd width=\"174\"\u003e203.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\"\u003ePNPG3\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e284.0\u003c\/td\u003e\n\u003ctd width=\"162\"\u003eMaltotetraose\u003c\/td\u003e\n\u003ctd width=\"174\"\u003e168.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"162\"\u003ePNPG5\u003c\/td\u003e\n\u003ctd width=\"162\"\u003e164.0\u003c\/td\u003e\n\u003ctd width=\"162\"\u003eMaltopentaose\u003c\/td\u003e\n\u003ctd width=\"174\"\u003e100.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e* : Substrate concn. 2.2mM\u003c\/p\u003e\n\u003cp\u003e** : Glucose-forming activity, pH 6.8 at 37℃\u003c\/p\u003e\n\u003cp\u003eEffect of various chemicals: (Table 1)\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003e\u003cu\u003eApplications\u003c\/u\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis enzyme is useful for structural investigations of carbohydrates and for the enzymatic determination of α-amylase when coupled with hexokinase and G-6-P dehydrogenase in clinical analysis.\u003c\/p\u003e\n\u003cp\u003e\u003c!--TBP0049-1KU TBP0049-5KU--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/02\/TBP0049-a-Glucosidase-1.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2024\/02\/TBP00049_a-Glucosidase-Safety-Data-Sheet.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1000U","offer_id":45986496610383,"sku":"TBP0049-1000U","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"5000U","offer_id":45986496643151,"sku":"TBP0049-5000U","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBP0049-1.png?v=1782812600","url":"https:\/\/www.danyel.co.il\/products\/a-glucosidase-enzyme-activity-tbp0049","provider":"danyel biotech","version":"1.0","type":"link"}