{"product_id":"10x-dna-loading-buffer-tbs5018","title":"10x DNA Loading Buffer","description":"\u003cp\u003eDNA Loading Buffer is a complete solution for the preparation of DNA samples prior to agarose gel electrophoresis and polyacrylamide gel electrophoresis. This optimized loading buffer prevents DNA degradation during the electrophoresis run.\u003c\/p\u003e\n\u003cp\u003eThe loading buffer contains two tracking dyes, bromophenol blue and Xylene cyanol that allow for tracking the progress of electrophoresis. In 1% agarose gels, Xylene cyanol typically migrates at about the same rate as a 4000 base pair DNA fragment, and bromophenol blue migrates at the same rate as a DNA fragment of approximately 500 base pairs. Xylene cyanol or tracking dye on a 6% polyacrylamide gel migrates at the speed of a 140 base pair DNA fragment. On 20% denaturating (7 M UREA) polyacrylamide PAGE gel electrophoresis Xylene cyanol migrates at about a rate of 25 base oligonucleotide.\u003c\/p\u003e\n\u003cp\u003eGlycerol is included in the loading buffer to ensure DNA samples remain in the gel wells after loading. 10 X loading buffer contains 0.1 M Tris-HCl, 0.2% SDS, 0.1% Bromophenol blue, 0.1% Xylene cyanol, 30% Glycerol.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eDirections for Use:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMix 1-volume DNA loading buffer with a 9-volume DNA sample, loading to agarose or polyacrylamide gel.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eStorage Conditions:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eSupplied as a 10X solution, 5x 1mL. Store at 4°C or room temperature.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 18px\"\u003e\u003cstrong\u003eRelated Products:\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/common-reagents\/tae-buffer-10x\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eTAE Buffer-10x (TBS5006)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/common-reagents\/te-buffer-1x\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eTE Buffer (TBS5009)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/nucleotide\/tissue-dna-extraction-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eTissue DNA Extraction kit (TBS6006)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/nucleotide\/mouse-tail-fast-dna-extraction-kit\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eMouse Tail DNA Extraction Kit (TBS6005)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/product\/life-sciences\/rt-pcr-kits\/tribo-2x-sybr-qpcr-super-mix\/\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eQPCR Super kit (TBS4001)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003c!--TBS5018--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS5018_10xDNA-loading-buffer-V02-2022.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/tbs5018-msds.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"Default Title","offer_id":45986334670927,"sku":"TBS5018","price":178.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS5023.jpg?v=1782808835","url":"https:\/\/www.danyel.co.il\/products\/10x-dna-loading-buffer-tbs5018","provider":"danyel biotech","version":"1.0","type":"link"}