{"product_id":"0-5m-tcep-hcl-solution-ph-6-0-7-0-and-8-0-tbs5082-01-6-0","title":"0.5M TCEP-HCl Solution (pH 6.0, 7.0 and 8.0)","description":"\u003cp\u003eTris(2-carboxyethyl) phosphine hydrochloride (TCEP) Solution is an extraordinarily powerful, odorless, thiol-free reducing agent with broad application to protein and nucleic acid involving the reduction of disulfide bonds. The pH value of the solution is designed at 6.0, 7.0, and 8.0 for multiple choices. This product is an effective and convenient replacement for βmercaptoethanol (β-ME) or DTT in protein analysis.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKit Contents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe product is supplied as a 0.5M solution at pH 6.0, 7.0, and 8.0.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eDirections for Use\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTCEP breaks disulfide bonds and can be used for a wide variety of protein applications including SDS-PAGE, mass spectrometry, labeling with cysteine specific tags, and modification of cysteine containing compounds. Its ability to prevent oxidation of protein samples makes it a useful buffer component because it helps to preserve enzymatic activity.\u003c\/p\u003e\n\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 18px\"\u003eKey Features\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eReplacement of DTT and β-ME:\u003c\/strong\u003e unlike DTT and βME, TCEP is odor-free, contributing to a healthier lab environment.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Efficient:\u003c\/strong\u003e 5 to 50mM TCEP thoroughly reduces most peptide or protein disulfide bonds within a few minutes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStable and versatile:\u003c\/strong\u003e reduces peptides and proteins over a broad range of pH, salt, detergent, and temperature conditions. It is resistant to air oxidation; nonvolatile and nonreactive toward other functional groups found in proteins.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCompatible:\u003c\/strong\u003e removal of this reagent is not necessary before most applications, (e.g. histidine-tagged protein purification, maleimide conjugations), because TCEP does not contain sulfhydryl groups.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cstrong style=\"font-size: 16px\"\u003e\u003cspan style=\"font-size: 18px\"\u003eStorage Conditions\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe product, as supplied, is stable for at least two years at 2-8℃ and shipped ambient.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003c!--TBS5082-01 TBS5082-01-6.0 TBS5082-01-7.0 TBS5082-01-8.0 TBS5082-10 TBS5082-10-6.0 TBS5082-10-7.0 TBS5082-10-8.0 TBS5082-50 TBS5082-50-6.0 TBS5082-50-7.0 TBS5082-50-8.0--\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 13px; font-weight: 700; text-transform: uppercase;\"\u003e\u003cstrong\u003eResearch use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/TBS5082_-TECEP_V03_2021.pdf\"\u003eDatasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/tribioscience.com\/wp-content\/uploads\/2019\/04\/tbs5082-msds.pdf\"\u003eMSDS (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"TriBioScience","offers":[{"title":"1 mL","offer_id":45986340733007,"sku":"TBS5082-01-6.0","price":114.0,"currency_code":"ILS","in_stock":true},{"title":"1 mL (TBS5082-01-7.0)","offer_id":45986340765775,"sku":"TBS5082-01-7.0","price":114.0,"currency_code":"ILS","in_stock":true},{"title":"1 mL (TBS5082-01-8.0)","offer_id":45986340798543,"sku":"TBS5082-01-8.0","price":114.0,"currency_code":"ILS","in_stock":true},{"title":"10 mL","offer_id":45986340831311,"sku":"TBS5082-10-6.0","price":864.0,"currency_code":"ILS","in_stock":true},{"title":"10 mL (TBS5082-10-7.0)","offer_id":45986340864079,"sku":"TBS5082-10-7.0","price":864.0,"currency_code":"ILS","in_stock":true},{"title":"10 mL (TBS5082-10-8.0)","offer_id":45986340896847,"sku":"TBS5082-10-8.0","price":864.0,"currency_code":"ILS","in_stock":true},{"title":"50 mL","offer_id":45986340929615,"sku":"TBS5082-50-6.0","price":3554.0,"currency_code":"ILS","in_stock":true},{"title":"50 mL (TBS5082-50-7.0)","offer_id":45986340962383,"sku":"TBS5082-50-7.0","price":3554.0,"currency_code":"ILS","in_stock":true},{"title":"50 mL (TBS5082-50-8.0)","offer_id":45986340995151,"sku":"TBS5082-50-8.0","price":3554.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TBS5082-01.jpg?v=1782809009","url":"https:\/\/www.danyel.co.il\/products\/0-5m-tcep-hcl-solution-ph-6-0-7-0-and-8-0-tbs5082-01-6-0","provider":"danyel biotech","version":"1.0","type":"link"}