{"title":"Takara Bio","description":"\u003cp\u003eThe Takara Bio group is a leading supplier of tools for life scientists worldwide.\u003c\/p\u003e\n\u003cp\u003eThrough our brand names TAKARA®, CLONTECH® and CELLARTIS™ we develop innovative technologies in the fields of Cell Biology, Molecular Biology, Proteomics and Stem Cell Research.\u003c\/p\u003e","products":[{"product_id":"primescript-rt-reagent-kit-perfect-real-time-rr037a","title":"PrimeScript™ RT Reagent Kit (Perfect Real Time)","description":"\u003cp\u003ePrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. This kit's protocol is simple and suitable for high throughput analysis. It can be used in combination with real-time PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A\/B), TB Green Fast qPCR Mix (Cat. #RR430A\/B), TB Green Premix Ex Taq (Tli RNaseH Pluse) (Cat. #RR420A\/B), or Probe qPCR Mix (Cat. #RR391A\/B) for two step real-time RT-PCR. The optimized protocol for assay can be selected in each assay condition using either TB Green or a probe.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAllows fast, efficient synthesis of cDNA for real-time PCR. This kit is designed for two-step real-time RT-PCR.\u003c\/li\u003e\n\u003cli\u003eIncludes random 6-mers and oligo dT primer for use as reverse transcription primers. The reaction can be performed using one or a mixture of these two primer types; alternately, gene-specific primers may be used.\u003c\/li\u003e\n\u003cli\u003eIncludes EASY Dilution (for Real Time PCR) when generating reference standards for a qPCR standard curve. This reagent facilitates higher accuracy at lower standard concentrations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/RR037A\/RR037A.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD2197\/SD2197-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/RR037A\/RR037A_UM.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"200 Rxns","offer_id":45986309374031,"sku":"RR037A","price":1876.0,"currency_code":"ILS","in_stock":true},{"title":"200 rxns","offer_id":45834320707663,"sku":"RR037A","price":1876.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/RR037A-PrimeScript-RT-Reagent-Kit-Perfect-Real-Time-product-600.jpg?v=1782808171"},{"product_id":"universal-buffer-set-1000","title":"Universal Buffer Set","description":"\u003cp\u003eUniversal Buffer Set 1 set\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"1 set","offer_id":45985995915343,"sku":"1000","price":533.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"uracil-dna-glycosylase-ung-heat-labile-2820","title":"Uracil DNA Glycosylase (UNG), Heat-Labile","description":"\u003cp\u003eAn enzyme that can help minimize contamination caused by carryover products from PCR amplification when used with dUTP.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/2820\/2820.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD3602\/SD3602-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/2820\/2820_DS.v1903Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"200 Units","offer_id":45985995980879,"sku":"2820","price":1928.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/2820-Uracil-DNA-Glycosylase-UNG-heat-labile-product-600.jpg?v=1782801001"},{"product_id":"lambda-dna-3010","title":"Lambda DNA","description":"\u003cp\u003eTakara's Lambda DNA is derived from an Escherichia coli bacteriophage. The double-stranded phage DNA is linear and contains 12 bp single-stranded complementary 5'-ends.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3010\/3010.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0423\/SD0423-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3010_DS.v1901Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"400 ug","offer_id":45985996046415,"sku":"3010","price":739.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3010-lambdaDNA-product-600.jpg?v=1782801003"},{"product_id":"phix174-rf-i-dna-3040","title":"PhiX174 RF I DNA","description":"\u003cp\u003eTakara's phiX174 RF I DNA is a double-stranded circular DNA molecule composed of 5,386 base pairs. Restriction enzyme digest of phiX174 RF I DNA results in the generation of molecular weight size markers that can be used in nucleic acid gel analysis. phiX174 double-stranded circular DNA is also frequently used in restriction enzyme quality control assays to assess endonuclease activity.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3040\/3040.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0426\/SD0426-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3040\/3040_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985996111951,"sku":"3040","price":648.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3040-phiX174-RF-I-DNA-product-600_763c086d-33dc-4e61-90bd-d80e4918d473.jpg?v=1782801005"},{"product_id":"pbr322-dna-3050","title":"pBR322 DNA","description":"\u003cp\u003epBR322 DNA is a commonly used plasmid cloning vector in E. coli.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEntry Name: SYNPBR322\u003c\/li\u003e\n\u003cli\u003eAccession No.: J01749\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3050\/3050.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985996177487,"sku":"3050","price":798.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3050-pBR322-DNA-product-600.jpg?v=1782801008"},{"product_id":"thermus-thermophilus-hb8-genomic-dna-solution-3071","title":"Thermus Thermophilus HB8 Genomic DNA Solution","description":"\u003cp\u003eThermus thermophilus HB8 Genomic DNA is a highly purified genomic DNA derived from Thermus thermophilus HB8 bacteria. It is extremely thermostable, because it is derived from extremely thermophilic bacterium that grows under high temperature conditions.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePrepared by phenol\/chloroform extraction\u003c\/li\u003e\n\u003cli\u003eA260\/A280: 1.8\/2.0\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3071\/3071.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0430\/SD0430-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3071\/3071_DS.v1901Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"2 ug","offer_id":45985996243023,"sku":"3071","price":2291.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3071-Thermus-thermophilus-HB8-Genomic-DNA-Solution-product-600.jpg?v=1782801010"},{"product_id":"pkf-18k-2-dna-3101","title":"pKF 18k-2 DNA","description":"\u003cp\u003eThe pKF pUC-derived vector DNA contains two amber stop mutations at the kanamycin resistance gene. When transformed with pKF, supE strains like JM109 but not sup0 strains like MV1184 will grow on kanamycin-containing plates. The pKF DNA may be used for site-directed mutagenesis based on the ODA (Oligonucleotide-directed Dual Amber) method. Using the simplified ODA procedure, a desired mutation can be introduced in only three days. Moreover, pKF DNA is available for target gene cloning and expression via the lac promoter when transformed into supE hosts like JM109. Target genes cloned at the initiation codon (ATG) of the Nde I restriction site (CATATG) have a higher translation efficiency.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3101\/3101_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45985996374095,"sku":"3101","price":620.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3101-pKF18k-2-DNA-product-600.jpg?v=1782801012"},{"product_id":"m13-mp18-rf-dna-3118","title":"M13 mp18 RF DNA","description":"\u003cp\u003eThe M13 mp18 RF Phage Vector is suitable for M13 sequencing by the dideoxy sequencing method. Since the phage vector contains a multiple cloning site in the lacZ' region, recombinant vectors can be easily verified via blue\/white colony screening using agar plates containing IPTG and X-Gal. The phage vector can also be directly used in M13 sequencing reactions because it is processed as a single-stranded DNA from phage particles.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3118\/3118.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0432\/SD0432-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3118\/3118_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45985996341327,"sku":"3118","price":818.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3118-M13-mp18-RF-DNA-product-600.jpg?v=1782801014"},{"product_id":"puc-18-dna-3218","title":"pUC 18 DNA","description":"\u003cp\u003eThis DNA is a plasmid vector suitable for DNA sequencing by dideoxy method. This DNA may allow successful cloning of DNA fragment longer than the case of M13 phage vector used. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, expression of the cloned DNA can be performed by using of lac promoter. M13 primers are available for DNA sequencing.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCloning and expression of target genes\u003c\/li\u003e\n\u003cli\u003eDNA sequencing using M13 primers\u003c\/li\u003e\n\u003cli\u003eLarge DNA insert sequencing using Takara Bio's Deletion Kit for Kilo-Sequencing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eCloning and expression of target genes \u003cbr\u003eDNA sequencing using M13 primers\u003cbr\u003eLarge DNA insert sequencing using Takara Bio's Deletion Kit for Kilo-Sequencing\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3218\/3218.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0433\/SD0433-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3218\/3218_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pUC18_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epUC18 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985996570703,"sku":"3218","price":537.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3218-pUC18-DNA-product-600.jpg?v=1782801021"},{"product_id":"puc-19-dna-3219","title":"pUC 19 DNA","description":"\u003cp\u003eThis DNA is a plasmid vector suitable for DNA sequencing by dideoxy method. It may allow successful cloning of DNA fragment longer than the case of M13 phage vector used. Since it has multi-cloning sites on lacZ' region, any DNA insertion into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, expression of the cloned DNA can be performed by using of lac promoter. M13 primers are available for DNA sequencing.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCloning and expression of target genes\u003c\/li\u003e\n\u003cli\u003eDNA sequencing using M13 primers\u003c\/li\u003e\n\u003cli\u003eLarge DNA insert sequencing using Takara Bio's Deletion Kit for Kilo-Sequencing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eCloning and expression of target genes \u003cbr\u003eDNA sequencing using M13 primers\u003cbr\u003eLarge DNA insert sequencing using Takara Bio's Deletion Kit for Kilo-Sequencing\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3219\/3219.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0433\/SD0433-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pUC18_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epUC18 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985996603471,"sku":"3219","price":541.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3219-pUC19-DNA-product-600.jpg?v=1782801022"},{"product_id":"pbapo-cmv-neo-dna-3240","title":"pBApo-CMV Neo DNA","description":"\u003cp\u003epBApo-CMV Neo is a plasmid for gene expression in mammalian cells. This vector has a promoter from cytomegalovirus (CMV IE promoter) and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site and, after transfection, stable integration of the vector can be selected for using G418.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003epIRES vectors include either puromycin, hygromycin, G418, or bleomycin resistance for selection\u003c\/li\u003e\n\u003cli\u003epBApo vectors include either neomycin or puromycin resistance for selection\u003c\/li\u003e\n\u003cli\u003eAllow for stable product expression in mammalian cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3240\/3240.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0441\/SD0441-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3240\/3240_3242_e.v1612Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pBApo-CMV%20Neo_Vector%20sequence%20file%20in.gb\"\u003epBApo-CMV Neo Vector sequence file in GenBank fomat\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631317\u003c\/td\u003e\n\u003ctd\u003eXfect™ Transfection Reagent\u003c\/td\u003e\n\u003ctd\u003e100 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45985996701775,"sku":"3240","price":2129.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3240-pBApo-CMV-Neo-DNA-product-600.jpg?v=1782801026"},{"product_id":"pbapo-cmv-pur-dna-3241","title":"pBApo-CMV Pur DNA","description":"\u003cp\u003epBApo-CMV Pur is a plasmid for gene expression in mammalian cells. This vector has a promoter from cytomegalovirus (CMV IE promoter) and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site and, after transfection, stable integration of the vector can be selected for using puromycin.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003epIRES vectors include either puromycin, hygromycin, G418, or bleomycin resistance for selection\u003c\/li\u003e\n\u003cli\u003epBApo vectors include either neomycin or puromycin resistance for selection\u003c\/li\u003e\n\u003cli\u003eAllow for stable product expression in mammalian cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3241\/3241.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0441\/SD0441-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pBApo-CMV%20Neo_Vector%20sequence%20file%20in.gb\"\u003epBApo-CMV Neo Vector sequence file in GenBank fomat\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631317\u003c\/td\u003e\n\u003ctd\u003eXfect™ Transfection Reagent\u003c\/td\u003e\n\u003ctd\u003e100 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45985996767311,"sku":"3241","price":2129.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3241-pBApo-CMV-Pur-DNA-product-600.jpg?v=1782801027"},{"product_id":"pbapo-cmv-dna-3242","title":"pBApo-CMV DNA","description":"\u003cp\u003epBApo-CMV is a plasmid for gene expression in mammalian cells. This vector has a promoter from cytomegalovirus (CMV IE promoter) and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003epIRES vectors include either puromycin, hygromycin, G418, or bleomycin resistance for selection\u003c\/li\u003e\n\u003cli\u003epBApo vectors include either neomycin or puromycin resistance for selection\u003c\/li\u003e\n\u003cli\u003eAllow for stable product expression in mammalian cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3242\/3242.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0441\/SD0441-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pBApo-CMV%20Neo_Vector%20sequence%20file%20in.gb\"\u003epBApo-CMV Neo Vector sequence file in GenBank fomat\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631317\u003c\/td\u003e\n\u003ctd\u003eXfect™ Transfection Reagent\u003c\/td\u003e\n\u003ctd\u003e100 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45985996832847,"sku":"3242","price":2034.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3242-pBApo-CMV-DNA-product-600.jpg?v=1782801031"},{"product_id":"pbapo-ef1alpha-neo-dna-3243","title":"pBApo-EF1alpha Neo DNA","description":"\u003cp\u003epBApo-EF1a Neo is a plasmid for gene expression in mammalian cells. This vector has a promoter from the human EF-1 alpha promoter and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site and, after transfection, stable integration of the vector can be selected for using G418.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003epIRES vectors include either puromycin, hygromycin, G418, or bleomycin resistance for selection\u003c\/li\u003e\n\u003cli\u003epBApo vectors include either neomycin or puromycin resistance for selection\u003c\/li\u003e\n\u003cli\u003eAllow for stable product expression in mammalian cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3243\/3243.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0441\/SD0441-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pBApo-CMV%20Neo_Vector%20sequence%20file%20in.gb\"\u003epBApo-CMV Neo Vector sequence file in GenBank fomat\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631317\u003c\/td\u003e\n\u003ctd\u003eXfect™ Transfection Reagent\u003c\/td\u003e\n\u003ctd\u003e100 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45985996898383,"sku":"3243","price":2129.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3243-pBApo-EF1alpha-Neo-DNA-product-600.jpg?v=1782801033"},{"product_id":"pbapo-ef1alpha-pur-dna-3244","title":"pBApo-EF1alpha Pur DNA","description":"\u003cp\u003epBApo-EF1a Pur is a plasmid for gene expression in mammalian cells. This vector has a promoter from the human EF-1 alpha promoter and polyA signal from herpes simplex virus thymidine kinase. A target gene can be cloned into multiple cloning site and, after transfection, stable integration of the vector can be selected for using puromycin.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003epIRES vectors include either puromycin, hygromycin, G418, or bleomycin resistance for selection\u003c\/li\u003e\n\u003cli\u003epBApo vectors include either neomycin or puromycin resistance for selection\u003c\/li\u003e\n\u003cli\u003eAllow for stable product expression in mammalian cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3244\/3244.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0441\/SD0441-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pBApo-CMV%20Neo_Vector%20sequence%20file%20in.gb\"\u003epBApo-CMV Neo Vector sequence file in GenBank fomat\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRequired Products\u003c\/h2\u003e\n\u003cp\u003e⚠️ A Required Product (e.g. an index kit) must be purchased to use this product — order one for each unit you buy.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCat. #\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e631317\u003c\/td\u003e\n\u003ctd\u003eXfect™ Transfection Reagent\u003c\/td\u003e\n\u003ctd\u003e100 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45985996963919,"sku":"3244","price":2129.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3244-pBApo-EF1alpha-Pur-DNA-product-600.jpg?v=1782801037"},{"product_id":"pri-909-dna-3260","title":"pRI 909 DNA","description":"\u003cp\u003epRI 909\/910 DNA are binary vectors that have a region of T-DNA for plant transformation. This originated from Ri plasmid of Rhizobium (Agrobacterium tumefaciens) rhizogenes, lacking a vir region. pRI 909\/910 DNA are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are propagated at high copy number due to the presence of ColE1 ori. pR1909\/910 are maintained stably in Rhizobium (Agrobacterium) due to mutant type replication origin of Ri plasmid (Ri-ori). These vectors include NPTIII to confer Kanamycin resistance as the selection marker for E. coli and Rhizobium (Agrobacterium), a mutant type kanamycin resistant gene NPTII as the selection marker for plant, and the same multi-cloning sites as pUC type plasmids.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEasy and stable plant chromosome target gene integration\u003c\/li\u003e\n\u003cli\u003eImproved agrobacterium-mediated plant transformation\u003c\/li\u003e\n\u003cli\u003eApplicable to various plants such as Arabidopsis, tomato, tobacco, and rice\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eRhizobium (Agrobacterium)-mediated plant transformation\u003cbr\u003eArabidopsis, tomato, tobacco, rice transformation\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3260\/3260.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0446\/SD0446-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3260\/3260_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pRI%20909%20DNA_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epRI 909 DNA Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45985996996687,"sku":"3260","price":3267.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3260-pRI-909-DNA-product-600.jpg?v=1782801037"},{"product_id":"pri-910-dna-3261","title":"pRI 910 DNA","description":"\u003cp\u003epRI 909\/910 DNA are binary vectors that have a region of T-DNA for plant transformation. This originated from Ri plasmid of Rhizobium (Agrobacterium tumefaciens) rhizogenes, lacking a vir region. pRI 909\/910 DNA are also shuttle vectors and replicate autonomously in E. coli and Rhizobium (Agrobacterium). In E. coli, these vectors are propagated at high copy number due to the presence of ColE1 ori. pR1909\/910 are maintained stably in Rhizobium (Agrobacterium) due to mutant type replication origin of Ri plasmid (Ri-ori). These vectors include NPTIII to confer Kanamycin resistance as the selection marker for E. coli and Rhizobium (Agrobacterium), a mutant type kanamycin resistant gene NPTII as the selection marker for plant, and the same multi-cloning sites as pUC type plasmids. The vectors are available for Agrobacterium-mediated binary plant transformation. Stable chromosomal integration of a target gene is possible because the cloning site is located at the position closer to Right Border (RB) of T-DNA than the selection marker (NPT II) for the plant, so the target gene is not deleted.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEasy and stable plant chromosome target gene integration\u003c\/li\u003e\n\u003cli\u003eImproved agrobacterium-mediated plant transformation\u003c\/li\u003e\n\u003cli\u003eApplicable to various plants such as Arabidopsis, tomato, tobacco, and rice\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eRhizobium (Agrobacterium)-mediated plant transformation\u003cbr\u003eArabidopsis, tomato, tobacco, rice transformation\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3261\/3261.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0446\/SD0446-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pRI%20909%20DNA_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epRI 909 DNA Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45985997094991,"sku":"3261","price":3267.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3261-pRI-910-DNA-product-600.jpg?v=1782801042"},{"product_id":"pri-101-an-dna-3262","title":"pRI 101-AN DNA","description":"\u003cp\u003epRI 101 DNA series are binary vectors for expressing foreign genes in plant cells, which include the 35S promoter of cauliflower mosaic virus (CaMV) and a 5' noncoding region (5'-UTR) of alcohol dehydrogenase (ADH) gene. The 5'-UTR of ADH functions as a translational enhancer in plants. There are two types of vectors, pRI 101-AN DNA having a 5'-UTR of Arabidopsis ADH (AtADH 5'-UTR) and pRI 101 ON DNA having a 5'-UTR of rice ADH (OsADH 5'-UTR). The pRI 101-AN is for dicotyledonous plants such as tobacco or Arabidopsis and the pRI 101-ON is for monocotyledonous plants such as rice. The pRI 101 DNA are shuttle vectors, and replicate autonomously in E. coli and Rhizobium (Agrobacterium).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAgrobacterium-mediated plant transformation\u003c\/li\u003e\n\u003cli\u003eEasy and stable integration of dicot and monocot target genes into plant chromosomes\u003c\/li\u003e\n\u003cli\u003eIncreased target gene expression via the 5'-UTR translation enhancer region\u003c\/li\u003e\n\u003cli\u003eVector versatility for dicotyledonous and monocotyledonous plant cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3262\/3262.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0448\/SD0448-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3262\/3262_3263_e.v1807Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pRI%20101-AN%20DNA_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epRI 101-AN DNA Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45985997160527,"sku":"3262","price":3267.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3262-pRI-101-AN-DNA-product-600.jpg?v=1782801044"},{"product_id":"pri-101-on-dna-3263","title":"pRI 101-ON DNA","description":"\u003cp\u003epRI 101 DNA series are binary vectors for expressing foreign genes in plant cells, which include the 35S promoter of cauliflower mosaic virus (CaMV) and a 5' noncoding region (5'-UTR) of alcohol dehydrogenase (ADH) gene. The 5'-UTR of ADH functions as a translational enhancer in plants. There are two types of vectors, pRI 101-AN DNA having a 5'-UTR of Arabidopsis ADH (AtADH 5'-UTR) and pRI 101 ON DNA having a 5'-UTR of rice ADH (OsADH 5'-UTR). The pRI 101-AN is for dicotyledonous plants such as tobacco or Arabidopsis and the pRI 101-ON is for monocotyledonous plants such as rice. The pRI 101 DNA are shuttle vectors, and replicate autonomously in E. coli and Rhizobium (Agrobacterium).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAgrobacterium-mediated plant transformation\u003c\/li\u003e\n\u003cli\u003eEasy and stable integration of dicot and monocot target genes into plant chromosomes\u003c\/li\u003e\n\u003cli\u003eIncreased target gene expression via the 5'-UTR translation enhancer region\u003c\/li\u003e\n\u003cli\u003eVector versatility for dicotyledonous and monocotyledonous plant cells\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3263\/3263.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0448\/SD0448-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pRI%20101-AN%20DNA_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epRI 101-AN DNA Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45985997226063,"sku":"3263","price":3267.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3263-pRI-101-ON-DNA-product-600.jpg?v=1782801047"},{"product_id":"pri-201-an-dna-3264","title":"pRI 201-AN DNA","description":"\u003cp\u003eUse pRI 201-AN DNA to transform dicotyledonous plants. Use pRI 201-ON DNA to transform monocotyledonous plants. The presence of 2 multicloning sites enable multigene transformation with a single vector. Vectors contain a replication origin (ColE1 ori) derived from pUC plasmids, which allows maintenance at a high-copy- number in E. coli.\u003cbr\u003epRI 201 DNA series are binary vectors designed for expressing target genes in transformed plant cells. This series of vectors retain the backbone of pRI 101 vectors (Cat.# 3262\/3263), which includes an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5' -UTR) (translational enhancer region) downstream of the 35S promoter from cauliflower mosaic virus (CaMV). In addition, they have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI 101 series 2. Further, multigene transformation with a single vector is possible by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the cloning site (MCS2) downstream of the HSP terminator.\u003cbr\u003eThe pRI 201 series has two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. Carrying an Arabidopsis ADH-derived 5' -UTR (AtADH 5' -UTR), pRI 201-AN DNA is suitable for dicotyledonous plants. With a rice ADH-derived 5' -UTR (OsADH 5' -UTR), pRI 201-ON DNA is compatible with monocotyledonous plants.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse pRI 201-AN DNA to transform dicotyledonous plant species\u003c\/li\u003e\n\u003cli\u003eUse pRI 201-ON DNA to transform monocotyledonous plant species\u003c\/li\u003e\n\u003cli\u003eIntegrate multiple transgenes using a single vector\u003c\/li\u003e\n\u003cli\u003eHigh copy number (ColE1 ori) replication in E. coli\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3264\/3264.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0450\/SD0450-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3264\/3264_DS.v1004Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pRI%20201-AN%20DNA_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epRI 201-AN DNA Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45985997258831,"sku":"3264","price":2544.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3264-pRI-201-AN-DNA-product-600.jpg?v=1782801048"},{"product_id":"pri-201-on-dna-3265","title":"pRI 201-ON DNA","description":"\u003cp\u003eUse pRI 201-AN DNA to transform dicotyledonous plants. Use pRI 201-ON DNA to transform monocotyledonous plants. The presence of 2 multicloning sites enable multigene transformation with a single vector. Vectors contain a replication origin (ColE1 ori) derived from pUC plasmids, which allows maintenance at a high-copy- number in E. coli. pRI 201 DNA series are binary vectors designed for expressing target genes in transformed plant cells. This series of vectors retain the backbone of pRI 101 vectors (Cat.# 3262\/3263), which includes an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5' -UTR) (translational enhancer region) downstream of the 35S promoter from cauliflower mosaic virus (CaMV). In addition, they have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI 101 series 2. Further, multigene transformation with a single vector is possible by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the cloning site (MCS2) downstream of the HSP terminator. The pRI 201 series has two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. Carrying an Arabidopsis ADH-derived 5' -UTR (AtADH 5' -UTR), pRI 201-AN DNA is suitable for dicotyledonous plants. With a rice ADH-derived 5' -UTR (OsADH 5' -UTR), pRI 201-ON DNA is compatible with monocotyledonous plants.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse pRI 201-AN DNA to transform dicotyledonous plant species\u003c\/li\u003e\n\u003cli\u003eUse pRI 201-ON DNA to transform monocotyledonous plant species\u003c\/li\u003e\n\u003cli\u003eIntegrate multiple transgenes using a single vector\u003c\/li\u003e\n\u003cli\u003eHigh copy number (ColE1 ori) replication in E. coli\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3265\/3265.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0450\/SD0450-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3264_3267_e.v2007Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pRI%20201-AN%20DNA_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epRI 201-AN DNA Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 ug","offer_id":45985997488207,"sku":"3265","price":2536.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3265-pRI-201-ON-DNA-product-600.jpg?v=1782801052"},{"product_id":"pri201-an-gus-dna-3266","title":"pRI201-AN-GUS DNA","description":"\u003cp\u003epRI201-AN-GUS DNA,10 ug\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"10 µg","offer_id":45985997455439,"sku":"3266","price":2457.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"pri201-on-gus-dna-3267","title":"pRI201-ON-GUS DNA","description":"\u003cp\u003epRI201-ON-GUS DNA,10 ug\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"10 µg","offer_id":45985997652047,"sku":"3267","price":2457.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"t-vector-pmd-20-3270","title":"T-Vector pMD™20","description":"\u003cp\u003eT-Vector pMD20 is a linearized vector with a single 3'-terminal thymidine at both ends. The T-overhang ends at the cloning site improves the efficiency of ligation of PCR products which contain A-overhangs at 3'-ends. T-Vector pMD20 is derived from a pUC19 backbone with modifications in the coding sequence to reduce the false positive rate and improve the positive colonies screened by blue\/white color on ampicillin\/IPTG\/X-Gal LB plates. In addition, an SP6 promoter has been inserted upstream of the multiple cloning site. Therefore, the inserted DNA fragments can be transcribed by SP6 RNA Polymerase (Cat. #2520). T-Vector pMD20 is supplied with a Control Insert (500 bp) DNA for positive control reactions.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eConcentration: 50 ng\/µl\u003c\/li\u003e\n\u003cli\u003eAmount: 1 µg\u003c\/li\u003e\n\u003cli\u003eVector size: pMD20 2,736 bp; pMD19 (Simple) 2,692 bp\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTA cloning\u003cbr\u003ePCR cloning\u003cbr\u003eSP6 expression (pMD20 only)\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3270\/3270.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0455\/SD0455-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3270\/3270_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/T-Vector%20pMD20%20%28Linear%29_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003eT-Vector pMD20 (Linear) Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 ug","offer_id":45985997848655,"sku":"3270","price":762.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3270-T-Vector-pMD20-product-600.jpg?v=1782801056"},{"product_id":"t-vector-pmd-19-simple-3271","title":"T-Vector pMD™19 (Simple)","description":"\u003cp\u003eT-Vector pMD19 (Simple) is a linearized vector with a single 3'-terminal thymidine at both ends. The T-overhang ends at the cloning site improve the efficiency of ligation of PCR products which contain A-overhangs at 3' -ends. The multiple cloning sites in the lac Z gene sequence have been deleted from this vector, but its β-galactosidase activity is not disrupted. Therefore, transformant colonies containing recombinant plasmids can be identified by blue\/white screening. Due to lack of multiple cloning sites in the vector, the inserted PCR product cannot be recut using restriction enzymes. When the insert DNA fragment is recut from the vector, the PCR primers should be used to add a restriction site to the 5'-end for restriction enzyme digestion and subcloning. T-Vector pMD19 (Simple) is supplied with a Control Insert (500 bp) DNA for positive control reactions.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eConcentration: 50 ng\/µl\u003c\/li\u003e\n\u003cli\u003eAmount: 1 µg\u003c\/li\u003e\n\u003cli\u003eVector size: pMD20 2,736 bp; pMD19 (Simple) 2,692 bp\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTA cloning\u003cbr\u003ePCR cloning\u003cbr\u003eSP6 expression (pMD20 only)\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3271\/3271.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0455\/SD0455-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/T-Vector%20pMD20%20%28Linear%29_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003eT-Vector pMD20 (Linear) Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 ug","offer_id":45985998307407,"sku":"3271","price":762.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3271-T-Vector-pMD19-Simple-product-600.jpg?v=1782801061"},{"product_id":"human-cell-free-protein-expression-system-3281","title":"Human Cell-Free Protein Expression System","description":"\u003cp\u003eThe Human Cell-Free Protein Expression System from Takara Bio is easy to use. The single-tube reaction is easily assembled and protein synthesis is complete in as little as 1 hr at 32°C. The Human Cell-Free Protein Expression System provides high yield (e.g., 50 μg\/ml of human eIF4G) of functional protein, including proteins requiring modifications such as glycosylation, phosphorylation, or fatty acylation and even with large proteins (over 150 kDa). Genes of interest may be cloned rapidly into pT7-IRES vectors using In-Fusion cloning technology. After expression, proteins with N-terminal or C-terminal His tag or N-terminal Myc tag can be generated, depending on choice of vector.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThey allow quick analyses of protein functions or features.\u003c\/li\u003e\n\u003cli\u003eThey are amenable to high-throughput studies.\u003c\/li\u003e\n\u003cli\u003eProteins that are degraded, insoluble, or toxic in in vivo systems can be easily expressed in vitro.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eExpression of toxic proteins that are lethal for in vivo expression systems\u003cbr\u003eQuick analysis of protein functionality\u003cbr\u003eRapid analysis of protein mutations or truncations\u003cbr\u003eExpression of proteins that are easily degraded or insoluble in conventional in vivo expression systems such as E. coli\u003cbr\u003eHigh-throughp\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3281\/3281.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD3654\/SD3654-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3281\/3281_e.v1807Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pT7-IRES_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epT7-IRES Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 Rxns","offer_id":45985998274639,"sku":"3281","price":2279.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3281-Human-Cell-Free-Protein-Expression-System-product-600.jpg?v=1782801061"},{"product_id":"pt7-ires-his-n-dna-3290","title":"pT7-IRES His-N DNA","description":"\u003cp\u003epT7-IRES DNA series are expression vectors designed for use with the Human Cell-Free Protein Expression System. pT7-IRES vectors contain tag sequences such as His-Tag or c-Myc Tag, Factor Xa cleavage site, Multiple Cloning Site (MCS), polyA, and T7 terminator are located at the downstream of a T7 promoter and an EMCV IRES. pT7-IRES His-N DNA is an expression vector including a his-tag sequence. Using pT7-IRES His-N DNA together with the Human Cell-Free Protein Expression System (Cat. #3281) enables the synthesis of your target protein as an N-terminal fusion with a his tag. A Factor Xa cleavage site is included so that the his tag can be removed from the synthesized fusion protein. Any target gene cloned into the MCS in frame is transcribed as an RNA-containing-EMCV IRES under the control of the T7 promoter. Efficient high level protein synthesis can be performed in the Human Cell-Free Protein Expression System, due to the effect of the EMCV IRES, which promotes protein translation initiation.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThey allow quick analyses of protein functions or features.\u003c\/li\u003e\n\u003cli\u003eThey are amenable to high-throughput studies.\u003c\/li\u003e\n\u003cli\u003eProteins that are degraded, insoluble, or toxic in in vivo systems can be easily expressed in vitro.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eExpression of toxic proteins that are lethal for in vivo expression systems\u003cbr\u003eQuick analysis of protein functionality\u003cbr\u003eRapid analysis of protein mutations or truncations\u003cbr\u003eExpression of proteins that are easily degraded or insoluble in conventional in vivo expression systems such as E. coli\u003cbr\u003eHigh-throughp\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD3654\/SD3654-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pT7-IRES_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epT7-IRES Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45985998405711,"sku":"3290","price":1051.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3290-pT7-IRES-His-N-DNA-product-600.jpg?v=1782801065"},{"product_id":"pt7-ires-his-c-dna-3291","title":"pT7-IRES His-C DNA","description":"\u003cp\u003epT7-IRES DNA series are expression vectors designed for use with the Human Cell-Free Protein Expression System. pT7-IRES vectors contain tag sequences such as His-Tag or c-Myc Tag, Factor Xa cleavage site, Multiple Cloning Site (MCS), polyA, and T7 terminator are located at the downstream of a T7 promoter and an EMCV IRES. pT7-IRES His-C DNA is an expression vector including a his-tag sequence. Using pT7-IRES His-C DNA together with the Human Cell-Free Protein Expression System (Cat. #3281) enables the synthesis of your target protein as a C-terminal fusion with a his tag. A Factor Xa cleavage site is included so that the his tag can be removed from the synthesized fusion protein. Any target gene cloned into the MCS in frame is transcribed as an RNA-containing-EMCV IRES under the control of the T7 promoter. Efficient high level protein synthesis can be performed in the Human Cell-Free Protein Expression System, due to the effect of the EMCV IRES, which promotes protein translation initiation.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThey allow quick analyses of protein functions or features.\u003c\/li\u003e\n\u003cli\u003eThey are amenable to high-throughput studies.\u003c\/li\u003e\n\u003cli\u003eProteins that are degraded, insoluble, or toxic in in vivo systems can be easily expressed in vitro.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eExpression of toxic proteins that are lethal for in vivo expression systems\u003cbr\u003eQuick analysis of protein functionality\u003cbr\u003eRapid analysis of protein mutations or truncations\u003cbr\u003eExpression of proteins that are easily degraded or insoluble in conventional in vivo expression systems such as E. coli\u003cbr\u003eHigh-throughp\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3291\/3291.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD3654\/SD3654-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pT7-IRES_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epT7-IRES Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45985998438479,"sku":"3291","price":1051.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3291-pT7-IRES-His-C-DNA-product-600.jpg?v=1782801065"},{"product_id":"pt7-ires-myc-n-dna-3292","title":"pT7-IRES Myc-N DNA","description":"\u003cp\u003epT7-IRES DNA series are expression vectors designed for use with the Human Cell-Free Protein Expression System. pT7-IRES vectors contain tag sequences such as His-Tag or c-Myc Tag, Factor Xa cleavage site, Multiple Cloning Site (MCS), polyA, and T7 terminator are located at the downstream of a T7 promoter and an EMCV IRES. pT7-IRES Myc-N DNA is an expression vector including a c-Myc tag sequence. Using pT7-IRES Myc-N DNA together with the Human Cell-Free Protein Expression System (Cat. #3281) enables the synthesis of your target protein as an N-terminal fusion with a c-Myc tag. A Factor Xa cleavage site is included so that the c-Myc tag can be removed from the synthesized fusion protein. Any target gene cloned into the MCS in frame is transcribed as an RNA-containing-EMCV IRES under the control of the T7 promoter. Efficient high level protein synthesis can be performed in the Human Cell-Free Protein Expression System, due to the effect of the EMCV IRES, which promotes protein translation initiation.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThey allow quick analyses of protein functions or features.\u003c\/li\u003e\n\u003cli\u003eThey are amenable to high-throughput studies.\u003c\/li\u003e\n\u003cli\u003eProteins that are degraded, insoluble, or toxic in in vivo systems can be easily expressed in vitro.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eExpression of toxic proteins that are lethal for in vivo expression systems\u003cbr\u003eQuick analysis of protein functionality\u003cbr\u003eRapid analysis of protein mutations or truncations\u003cbr\u003eExpression of proteins that are easily degraded or insoluble in conventional in vivo expression systems such as E. coli\u003cbr\u003eHigh-throughp\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3292\/3292.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD3654\/SD3654-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pT7-IRES_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epT7-IRES Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 ug","offer_id":45985998569551,"sku":"3292","price":1039.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3292-pT7-IRES-Myc-N-DNA-product-600.jpg?v=1782801073"},{"product_id":"phsg-298-dna-3298","title":"pHSG 298 DNA","description":"\u003cp\u003epHSG298 is a kanamycin-resistant pUC cloning vector which contains the same consensus multicloning site as pUC18 (though Hind III and Sma I sites are not available). Using this vector, it is possible to transfer a DNA fragment from pUC19 to pUC18 without the preparation of DNA fragments.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003elac promoter-enabled target gene cloning and expression\u003c\/li\u003e\n\u003cli\u003eDNA sequencing using an M13 primer\u003c\/li\u003e\n\u003cli\u003eLong DNA sequencing using the Deletion Kit for Kilo-Sequencing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003elac promoter-enabled target gene cloning and expression\u003cbr\u003eDNA sequencing using an M13 primer\u003cbr\u003eLong DNA sequencing using the Deletion Kit for Kilo-Sequencing\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3298\/3298.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0457\/SD0457-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3298\/3298_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pHSG298_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epHSG298 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985998536783,"sku":"3298","price":541.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3298-pHSG-298-DNA-product-600.jpg?v=1782801071"},{"product_id":"phsg-299-dna-3299","title":"pHSG 299 DNA","description":"\u003cp\u003epHSG299 is a kanamycin-resistant pUC cloning vector which contains the same consensus multicloning site as pUC19 (though Hind III and Sma I sites are not available). Using this vector, it is possible to transfer a DNA fragment from pUC18 to pUC19 without the preparation of DNA fragments.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003elac promoter-enabled target gene cloning and expression\u003c\/li\u003e\n\u003cli\u003eDNA sequencing using an M13 primer\u003c\/li\u003e\n\u003cli\u003eLong DNA sequencing using the Deletion Kit for Kilo-Sequencing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003elac promoter-enabled target gene cloning and expression\u003cbr\u003eDNA sequencing using an M13 primer\u003cbr\u003eLong DNA sequencing using the Deletion Kit for Kilo-Sequencing\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3299\/3299.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0457\/SD0457-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pHSG298_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epHSG298 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985998700623,"sku":"3299","price":541.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3299-pHSG-299-DNA-product-600.jpg?v=1782801076"},{"product_id":"puc-118-dna-3318","title":"pUC 118 DNA","description":"\u003cp\u003epUC118 is a plasmid vector constructed by inserting a HgiA I (5645) - DraI (5941) fragment containing the intergenic region (IG region) of the M13 phage DNA into the NdeI site of the pUC18 plasmid. Since it has multi-cloning sites in the lacZ region, any DNA inserted into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, the cloned DNA can be expressed by using the lac promoter.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eConcentration: 0.5 µg\/µl\u003c\/li\u003e\n\u003cli\u003eForm: 10 mM Tris-HCI (pH8.0), 1 mM EDTA\u003c\/li\u003e\n\u003cli\u003eChain Length: 3,162 bp\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eCloning of a target gene\u003cbr\u003eDNA sequencing using M13 primers\u003cbr\u003eDetermination of long DNA sequences using Deletion Kit for Kilo-Sequencing (Cat. # 6030)\u003cbr\u003eGene expression using lac promoter\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3318\/3318.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0459\/SD0459-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3318\/3318_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pUC118_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epUC118 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985998667855,"sku":"3318","price":537.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3318-pUC118-DNA-product-600.jpg?v=1782801077"},{"product_id":"puc-118-ecor-i-bap-3320","title":"pUC 118 EcoR I\/BAP","description":"\u003cp\u003eThis cloning vector was prepared by cleavage with EcoRI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3320\/3320.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"5 ug","offer_id":45985998864463,"sku":"3320","price":703.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3320-pUC118-EcoR-I-BAP-product-600.jpg?v=1782801082"},{"product_id":"puc-119-dna-3319","title":"pUC 119 DNA","description":"\u003cp\u003epUC119 is a plasmid vector constructed by inserting a HgiA I(5645) - DraI(5941) fragment containing the intergenic region (IG region) of the M13 phage DNA into the NdeI site of the pUC19 plasmid. Since its multiple cloning site is in the lacZ region, any DNA inserted into this vector can be easily verified using plates containing IPTG and X-Gal. Moreover, the cloned DNA can be expressed using the lac promoter.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eConcentration: 0.5 µg\/µl\u003c\/li\u003e\n\u003cli\u003eForm: 10 mM Tris-HCI (pH8.0), 1 mM EDTA\u003c\/li\u003e\n\u003cli\u003eChain Length: 3,162 bp\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eCloning of a target gene\u003cbr\u003eDNA sequencing using M13 primers\u003cbr\u003eDetermination of long DNA sequences using Deletion Kit for Kilo-Sequencing (Cat. # 6030)\u003cbr\u003eGene expression using lac promoter\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3319\/3319.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0459\/SD0459-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pUC118_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epUC118 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985998929999,"sku":"3319","price":608.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3319-pUC-119-DNA-product-600_ea8af467-a128-4a14-8b45-58be26b52920.jpg?v=1782801082"},{"product_id":"puc-118-bamh-i-bap-3321","title":"pUC 118 BamH I\/BAP","description":"\u003cp\u003eThis cloning vector was prepared by cleavage with BamHI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3321\/3321.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"5 ug","offer_id":45985998995535,"sku":"3321","price":608.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3321-pUC118-BamH-I-BAP-product-600.jpg?v=1782801086"},{"product_id":"puc-118-hinc-ii-bap-3322","title":"pUC 118 Hinc II\/BAP","description":"\u003cp\u003eThis cloning vector was prepared by cleavage with HincII (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3322\/3322.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"5 ug","offer_id":45985999061071,"sku":"3322","price":608.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3322-pUC-118-Hinc-IIBAP-product-600.jpg?v=1782801089"},{"product_id":"puc-118-pst-i-bap-3323","title":"pUC 118 Pst I\/BAP","description":"\u003cp\u003eThis cloning vector was prepared by cleavage with PstI (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3323\/3323.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"5 ug","offer_id":45985999126607,"sku":"3323","price":604.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3323-pUC118-Pst-I-BAP-product-600.jpg?v=1782801091"},{"product_id":"puc-118-hind-iii-bap-3324","title":"pUC 118 Hind III\/BAP","description":"\u003cp\u003eThis cloning vector was prepared by cleavage with HindIII (which has a unique site within the multiple cloning site of pUC118) followed by dephosphorylation with alkaline phosphatase purified from E. coli (BAP). This DNA is able to prevent self-ligation of vectors, and also able to decrease blank value of transforming cells. Thus, this DNA is available for cloning without other treatment.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3324\/3324.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"5 ug","offer_id":45985999192143,"sku":"3324","price":608.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3324-pUC-118-Hind-IIIBAP-product-600.jpg?v=1782801093"},{"product_id":"ptv-118n-dna-0-5-od-3328","title":"pTV 118N DNA (0.5 OD)","description":"\u003cp\u003epTV118N DNA contains the sequence CCATGC, which is the NcoI cleavage site and contains the initiation codon (ATG) of lacZ. When a target gene is inserted into the NcoI site, induction and expression of the gene will be under control of the lac promoter and SD sequence of lacZ. This vector is designed to have a distance of 8 bases between the SD sequence of lacZ and the starting codon, in order to improve the efficiency of translational initiation. The translation frame can be confirmed by sequencing the starting codon region using M13 primer RV-N after isolation of the single-strand DNA using helper phage. Note: It is not possible to sequence the single-stranded DNA using a typical M13 forward primer, because the M13 IG region for lacZ in pTV118N is reverse-oriented relative to pUC118.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3328\/3328.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985999257679,"sku":"3328","price":620.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3328-pTV-118N-DNA-0-point-5-OD-product-600.jpg?v=1782801096"},{"product_id":"pstv-28-dna-3331","title":"pSTV 28 DNA","description":"\u003cp\u003epSTV 28 DNA is a plasmid vector reconstructed from the β-galactosidase gene, with the replication origin of pACYC184 (the chloramphenicol resistance gene for Tn9). There is a pUC118 multicloning site but XbaI and AccI sites are not available. Since this DNA has lower copy numbers relative to pUC-type vectors, it is useful for cloning genes which do harm to the host if expressed in large amounts. Moreover, since this DNA contains a pACYC184 ori, it can be present into the same cell with plasmid vectors such as pUC and pBR.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3331\/3331.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985999290447,"sku":"3331","price":703.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3331-pSTV28-DNA-product-600.jpg?v=1782801098"},{"product_id":"pstv-29-dna-3332","title":"pSTV 29 DNA","description":"\u003cp\u003epSTV 29 DNA is a plasmid vector reconstructed from the β-galactosidase gene, with the replication origin of pACYC184 (the chloramphenicol resistance gene for Tn9). There is a pUC119 multicloning site but XbaI and AccI sites are not available. Since this DNA has lower copy numbers relative to pUC-type vectors, it is useful for cloning genes which do harm to the host if expressed in large amounts. Moreover, since this DNA contains a pACYC184 ori, it can be present into the same cell with plasmid vectors such as pUC and pBR.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3332\/3332.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0431\/SD0431-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985999388751,"sku":"3332","price":711.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3332-pSTV29-DNA-product-600.jpg?v=1782801102"},{"product_id":"chaperone-plasmid-set-3340","title":"Chaperone Plasmid Set","description":"\u003cp\u003eThe Chaperone Plasmid Set consists of five different plasmids, each of which is designed to express multiple molecular chaperones that function as a \"chaperone team\" to enable protein folding. Co-expression of a target protein with one of these chaperone teams increases the recovery of soluble proteins. Each plasmid carries an origin of replication derived from pACYC and a Cmr gene, which allows use with E. coli expression systems utilizing ColE1-type plasmids containing an ampicillin resistance gene as a marker. The chaperone genes are situated downstream of an araB or Pzt-1 (tet) promoter. Therefore, expression of target proteins and chaperones can be induced individually if the target gene is placed under the control of other promoters (e.g. lac). These plasmids also contain the necessary regulator (araC or tetr) for each promoter.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCorrect in vivo folding of expressed recombinant proteins in E. coli\u003c\/li\u003e\n\u003cli\u003eReduction of insoluble protein during expression\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eCorrect in vivo folding of expressed recombinant proteins in E. coli\u003cbr\u003eReduction of insoluble protein during expression\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3340\/3340.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0469\/SD0469-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3340\/3340_e.v1701Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pG-KJE8_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epG-KJE8 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pG-Tf2_Vector%20file%20sequence%20in%20.gb%20format.gb\"\u003epG-Tf2 Vector file sequence in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pGro7_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epGro7 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pKJE7_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epKJE7 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/pTf16_Vector%20sequence%20file%20in%20.gb%20format.gb\"\u003epTf16 Vector sequence file in GenBank format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 Set","offer_id":45985999421519,"sku":"3340","price":1797.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3340-Chaperone-Plasmid-Set-product-600.jpg?v=1782801102"},{"product_id":"pcold-vector-set-3360","title":"pCold™ Vector Set","description":"\u003cp\u003eTakara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, three of the vectors also contain either a translation enhancing element (TEE), His-Taq sequence, and\/or Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat.# 3340).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpression of difficult proteins that can not be expressed with the T7 system\u003c\/li\u003e\n\u003cli\u003eIncreased solubility due to expression at reduced temperature\u003c\/li\u003e\n\u003cli\u003eIncreased purity due to repressed expression of host proteins\u003c\/li\u003e\n\u003cli\u003eWhen used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased\u003c\/li\u003e\n\u003cli\u003eRadioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHigh-efficiency protein expression using a cold-shock promoter\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3360\/3360.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0478\/SD0478-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3360\/3360-3364_e.v1807Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 Set","offer_id":45985999716431,"sku":"3360","price":8216.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3360-pCold-Vector-Set-product-600.jpg?v=1782801106"},{"product_id":"pcold-i-dna-3361","title":"pCold™ I DNA","description":"\u003cp\u003eTakara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli.  These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold I DNA contains a translation enhancing element (TEE), his-tag sequence, and a Factor Xa cleavage site. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat. # 3340).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpression of difficult proteins that can not be expressed with the T7 system\u003c\/li\u003e\n\u003cli\u003eIncreased solubility due to expression at reduced temperature\u003c\/li\u003e\n\u003cli\u003eIncreased purity due to repressed expression of host proteins\u003c\/li\u003e\n\u003cli\u003eWhen used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased\u003c\/li\u003e\n\u003cli\u003eRadioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHigh-efficiency protein expression using a cold-shock promoter\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3361\/3361.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0478\/SD0478-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985999781967,"sku":"3361","price":2860.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3361-pCold-I-DNA-product-600.jpg?v=1782801108"},{"product_id":"pcold-ii-dna-3362","title":"pCold™ II DNA","description":"\u003cp\u003eTakara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore, pCold II DNA contains a translation enhancing element (TEE) and his-tag sequence. These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat. # 3340).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpression of difficult proteins that can not be expressed with the T7 system\u003c\/li\u003e\n\u003cli\u003eIncreased solubility due to expression at reduced temperature\u003c\/li\u003e\n\u003cli\u003eIncreased purity due to repressed expression of host proteins\u003c\/li\u003e\n\u003cli\u003eWhen used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased\u003c\/li\u003e\n\u003cli\u003eRadioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHigh-efficiency protein expression using a cold-shock promoter\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3362\/3362.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0478\/SD0478-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985999847503,"sku":"3362","price":2974.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3362-pCold-II-DNA-product-600_50df317c-8c01-445b-aa45-84c7716de03d.jpg?v=1782801111"},{"product_id":"pcold-iii-dna-3363","title":"pCold™ III DNA","description":"\u003cp\u003eTakara's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). Furthermore,pCold III DNA contains a translation enhancing element (TEE). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara's Chaperone Plasmid Set (Cat. # 3340).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpression of difficult proteins that can not be expressed with the T7 system\u003c\/li\u003e\n\u003cli\u003eIncreased solubility due to expression at reduced temperature\u003c\/li\u003e\n\u003cli\u003eIncreased purity due to repressed expression of host proteins\u003c\/li\u003e\n\u003cli\u003eWhen used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased\u003c\/li\u003e\n\u003cli\u003eRadioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHigh-efficiency protein expression using a cold-shock promoter\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3363\/3363.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0478\/SD0478-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985999814735,"sku":"3363","price":2974.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3363-pCold-III-DNA-product-600.jpg?v=1782804127"},{"product_id":"pcold-iv-dna-3364","title":"pCold™ IV DNA","description":"\u003cp\u003eTakara Bio's pCold Expression Vectors offer cold shock expression technology for high purity, high yield protein production. The series includes four different vectors that utilize the cold shock Protein A (cspA) promoter to direct expression of recombinant protein in E. coli. These vectors selectively induce target protein synthesis at low temperatures (15°C) when the synthesis of other proteins is suppressed and protease activity is decreased. This results in high yield of the target protein (60% of intracellular protein). In addition to the cspA promoter, all four vectors contain a lac operator (for control of expression), ampicillin resistance gene (ampr), ColE1 origin of replication, M13 IG fragment, and multiple cloning site (MCS). These vectors work equally well for synthesis of non-labeled and radiolabeled proteins and can be used in conjunction with Takara Bio's Chaperone Plasmid Set (Cat. # 3340).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eExpression of difficult proteins that can not be expressed with the T7 system\u003c\/li\u003e\n\u003cli\u003eIncreased solubility due to expression at reduced temperature\u003c\/li\u003e\n\u003cli\u003eIncreased purity due to repressed expression of host proteins\u003c\/li\u003e\n\u003cli\u003eWhen used in conjunction with one of our Chaperone Plasmids, the amount of recoverable soluble protein can be further increased\u003c\/li\u003e\n\u003cli\u003eRadioisotope labeling: Up to 90% of newly expressed cellular protein is labeled target protein\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eHigh-efficiency protein expression using a cold-shock promoter\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3364\/3364.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0478\/SD0478-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45985999978575,"sku":"3364","price":2974.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3364-pCold-IV-DNA-product-600.jpg?v=1782801120"},{"product_id":"pcold-tf-dna-3365","title":"pCold™ TF DNA","description":"\u003cp\u003eThe pCold TF DNA Vector provides cold shock technology for high-yield protein expression combined with Trigger Factor (chaperone) expression to facilitate correct protein folding, thus enabling efficient soluble protein production for otherwise intractable target proteins. pCold TF DNA Vector is a fusion cold shock expression vector that expresses Trigger Factor (TF) chaperone as a soluble tag. Trigger Factor is a prokaryotic ribosome-associated chaperone protein (48 kDa) that facilitates co-translational folding of newly expressed polypeptides. Because of its E. coli origin, TF is highly expressed in E. coli expression systems. The pCold TF DNA Vector consists of the cspA promoter plus additional downstream sequences, including a 5' untranslated region (5' UTR), a translation enhancing element (TEE), a His-Tag sequence, and a multiple cloning site (MCS). A lac operator is inserted downstream of the cspA promoter to ensure strict regulation of expression. Additionally, recognition sites for HRV 3C Protease, Thrombin, and Factor Xa are located between TF-Tag and the Multiple Cloning Site (MCS) and facilitate tag removal from the expressed fusion protein. E. coli strains can serve as expression hosts.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHighly efficient protein expression using cold shock technology\u003c\/li\u003e\n\u003cli\u003eHigh yield of active protein due to the trigger factor chaperone solubility-promoting fusion tag\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/3365\/3365.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0482\/SD0482-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/3365\/3365_e.v1909Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 ug","offer_id":45986000044111,"sku":"3365","price":5502.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/3365-pCold-TF-DNA-product-600.jpg?v=1782801124"},{"product_id":"spp-system-i-3367","title":"SPP System™ I","description":"\u003cp\u003eSPP System I,1 set\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"1 Set","offer_id":45986001453135,"sku":"3367","price":6281.0,"currency_code":"ILS","in_stock":true}]}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/collections\/takara-logo.png?v=1780549956","url":"https:\/\/www.danyel.co.il\/collections\/takara-bio.oembed","provider":"danyel biotech","version":"1.0","type":"link"}