{"title":"Reverse Transcription \u0026 cDNA Synthesis","description":"\u003cp\u003ePrimeScript and SMARTer kits, reverse transcriptases and RT master mixes from Takara Bio — shipped and supported from within Israel.\u003c\/p\u003e","products":[{"product_id":"amersham-ready-to-go-rt-pcr-beads-27926701","title":"Amersham Ready-To-Go RT-PCR Beads","description":"\u003cp\u003ePre-dispensed, freeze-dried beads that include the reagents necessary for one-step reverse transcription-PCR with high sensitivity and reproducibility.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePreformulated, predispensed, single-dose, ambient-temperature-stable beads ensure greater reproducibility between reactions, minimize pipetting steps, and reduce the potential for pipetting errors and contamination.\u003c\/li\u003e\n\u003cli\u003eOptimized as a one-tube, one-step RT-PCR reaction for both cDNA synthesis and PCR.\u003c\/li\u003e\n\u003cli\u003eEach lot of RT-PCR Beads is function tested for its ability to generate highly specific PCR products to ensure lot-to-lot reproducibility\u003c\/li\u003e\n\u003cli\u003eFor highly reproducible one-step reverse transcription-PCR.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eDescription\u003c\/p\u003e\u003cp\u003eReady-To-Go RT-PCR Beads are stable at room temperature and designed for performing single-tube one-step reverse transcription-PCR.  Each room-temperature-stable bead contains M-MuLV Reverse Transcriptase, RNase Inhibitor, buffer, nucleotides, and \u003ci\u003eTaq\u003c\/i\u003e DNA Polymerase. The only additional reagents required are water, template RNA, and primers. The reagents are optimized for full-length cDNA synthesis to \u0026gt;7.5 kb and optimal sensitivity from PCR.\u003c\/p\u003e\u003cp\u003eThe Ready-To-Go Bead format significantly reduces the number of pipetting steps, thereby increasing reproducibility of the RT-PCR technique and minimizing risk of contamination and RNA degradation. Ready-To-Go RT-PCR Beads are provided in either thin walled 0.5 ml or 0.2 ml tubes compatible with most thermocyclers. The 0.2 ml tubes come assembled in a 96-well (8 × 12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of either the entire 96-well plate, strips of eight or individual 0.2 ml tubes. Each package of Ready-To-Go RT-PCR Beads contains: RT-PCR beads, control reactions and pd(N)6 and oligo(dT) cDNA primers.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-51116-pdf\"\u003eData file (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"0.2 ml Hinged Tube with Cap","offer_id":45982269833295,"sku":"27925901","price":2848.0,"currency_code":"ILS","in_stock":true},{"title":"0.2 ml Tubes","offer_id":45982269866063,"sku":"27926701","price":2858.0,"currency_code":"ILS","in_stock":true},{"title":"0.5 ml Tubes","offer_id":45982269898831,"sku":"27926601","price":2928.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-31391-original-png_0fbc8034-d013-4f42-9eba-c501ed95647e.png?v=1785959656"},{"product_id":"ready-to-go-you-prime-first-strand-beads-27926401","title":"Ready-To-Go You-Prime First-Strand Beads","description":"\u003cp\u003eThe kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 ml tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice for RT-PCR.\u003c\/li\u003e\n\u003cli\u003ePredispensed, ambient-temperature-stable, single-dose reaction beads minimize pipetting errors, avoid cross-contamination, and ensure optimal performance.\u003c\/li\u003e\n\u003cli\u003eFirst-strand reaction beads contain no primer, thus allowing users to select a first-strand primer of choice.\u003c\/li\u003e\n\u003cli\u003eHighly suitable for research applications that use PCR to detect and quantify eukaryotic RNA from a variety of samples.\u003c\/li\u003e\n\u003cli\u003eReaction beads are function-tested for first-strand synthesis of cDNA up to 7.5 kb and in RT-PCR from blood samples.\u003c\/li\u003e\n\u003cli\u003eCompleted reactions (33 µl final volume) can be used directly in PCR after adding water, Taq DNA polymerase, and primers; first-strand cDNA can also be used as a template for traditional Gubler-Hoffman second-strand cDNA synthesis.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach kit contains predispensed first-strand reaction mixes which are treated by a special process turning each one into two small beads, stable at ambient temperatures. Each first-strand reaction is quickly reconstituted when mixed with an aqueous solution. Thus, with Ready-To-Go You-Prime First-Strand Beads, preparation and setup are convenient. The beads are reconstituted with the denatured RNA sample, primer is added, and the reaction is placed at 37°C for 60 minutes. The completed first-strand reaction is then available for PCR amplification or second-strand cDNA synthesis.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/27926401PL.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"50 reactions","offer_id":45982292639823,"sku":"27926401","price":3186.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-9523-original-png_2ddda3f4-14d6-4110-9229-c485a4a914a4.png?v=1785960038"},{"product_id":"first-strand-cdna-synthesis-kit-27926101","title":"First-Strand cDNA Synthesis Kit","description":"\u003cp\u003eFirst-Strand cDNA Synthesis kits are designed to generate full-length first-strand cDNA from mRNA templates following upstream mRNA extraction, isolation, or purification.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eFor synthesis of single-stranded cDNA prior to amplification by PCR\u003c\/li\u003e\n\u003cli\u003ePreassembled bulk reaction mixes save time and minimize potential for pipetting errors\u003c\/li\u003e\n\u003cli\u003eAllows users to choose primers\u003c\/li\u003e\n\u003cli\u003eIncludes a choice of two primers: pd(N)6 random hexamers and NotI-(dT)18\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFirst-Strand cDNA Synthesis Kit\u003c\/p\u003e\n\n\u003cp\u003eAfter first-strand cDNA synthesis, the sample can be used directly for \u003cem\u003ein vitro\u003c\/em\u003e amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method.\u003c\/p\u003e\n\n\u003cp\u003ePrimers are not included in the preassembled First-Strand Reaction Mixes, thus allowing use of a primer of choice.\u003c\/p\u003e\n\n\u003cp\u003eTwo primers are included with the kit. pd(N)\u003csub\u003e6\u003c\/sub\u003e Primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5'-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail.\u003c\/p\u003e\n\n\u003cp\u003eThe NotI-(dT)18 Bifunctional Primer can be used to prime selectively on mRNA that has a poly(A) tail. It can also be used as a PCR primer following first-strand synthesis. The NotI restriction site, located at the 5'-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products.\u003c\/p\u003e\n\n\u003cp\u003eFirst-Strand cDNA Synthesis Kit contains the following reagents, sufficient for up to 55 first-strand syntheses, each of which will produce enough material for up to 15 amplification reactions: Bulk First-Strand cDNA Reaction Mixes, mRNA Standard, DTT Solution, RNase-Free Water, pd(N)\u003csub\u003e6\u003c\/sub\u003e Primer, NotI-(dT)18 Bifunctional Primer, and instruction booklet.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/27926101.pdf\"\u003eInstructions for use (PDF)\u003c\/a\u003e\u003c\/li\u003e\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"55 reactions","offer_id":45982298210383,"sku":"27926101","price":2737.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/digi-31350-original-png_fcc183ed-2b7c-4d1e-839c-ec5783b85411.png?v=1785960177"},{"product_id":"oligo-dt-15-primer-3805","title":"Oligo (dT)15 Primer","description":"\u003cp\u003eOligo (dT)15 Primer,8 nmol\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"8 nmol","offer_id":45986005024847,"sku":"3805","price":624.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"atp-4041","title":"ATP","description":"\u003cp\u003eTakara's Ribonucleoside Triphosphates are ≥ 95% pure and available in a convenient 100 mM concentration.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIn vitro transcription\u003c\/li\u003e\n\u003cli\u003esiRNA synthesis for RNAi studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eIn vitro transcription \u003cbr\u003esiRNA synthesis for RNAi studies\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/4041\/4041.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0715\/SD0715-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/4041\/4041_DS.v1812Da.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 umol","offer_id":45986005778511,"sku":"4041","price":209.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/4041-ATP-product-600.jpg?v=1782801609"},{"product_id":"gtp-4042","title":"GTP","description":"\u003cp\u003eTakara's Ribonucleoside Triphosphates are ≥ 95% pure and available in a convenient 100 mM concentration.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIn vitro transcription\u003c\/li\u003e\n\u003cli\u003esiRNA synthesis for RNAi studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eIn vitro transcription \u003cbr\u003esiRNA synthesis for RNAi studies\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/4042\/4042.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0715\/SD0715-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 umol","offer_id":45986005745743,"sku":"4042","price":209.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/4042-GTP-product-600.jpg?v=1782801609"},{"product_id":"ctp-4043","title":"CTP","description":"\u003cp\u003eTakara's Ribonucleoside Triphosphates are ≥ 95% pure and available in a convenient 100 mM concentration.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIn vitro transcription\u003c\/li\u003e\n\u003cli\u003esiRNA synthesis for RNAi studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eIn vitro transcription \u003cbr\u003esiRNA synthesis for RNAi studies\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/4043\/4043.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0715\/SD0715-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 umol","offer_id":45986006073423,"sku":"4043","price":209.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/4043-CTP-product-600.jpg?v=1782801613"},{"product_id":"utp-4044","title":"UTP","description":"\u003cp\u003eTakara's Ribonucleoside Triphosphates are ≥ 95% pure and available in a convenient 100 mM concentration.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eIn vitro transcription\u003c\/li\u003e\n\u003cli\u003esiRNA synthesis for RNAi studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eIn vitro transcription \u003cbr\u003esiRNA synthesis for RNAi studies\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/4044\/4044.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD0715\/SD0715-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"25 umol","offer_id":45986006171727,"sku":"4044","price":209.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/4044-UTP-product-600.jpg?v=1782801618"},{"product_id":"takara-ivtpro-mrna-synthesis-system-low-dsrna-6131","title":"Takara IVTpro™ mRNA Synthesis System (low dsRNA)","description":"\u003cp\u003eThe Takara IVTpro mRNA Synthesis System (low dsRNA) includes all the\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStreamlined workflow—complete in vitro transcription workflow, from template preparation to mRNA purification, with a single kit\u003c\/li\u003e\n\u003cli\u003eUltra-high yield—generate up to 200 µg of mRNA per reaction with a cap structure and poly(A) sequence\u003c\/li\u003e\n\u003cli\u003eFlexibility—incorporate a wide variety of capping systems, including CleanCap or ARCA and\/or a modified nucleoside such as pseudouridine\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eConstruction of plasmid DNA for in vitro transcription template\u003cbr\u003eCapped mRNA synthesis using TriLink's CleanCap Reagent AG, or other cap analogs such as Anti-Reverse Cap Analog (ARCA)*\u003cbr\u003eSynthesis of long non-coding RNA or siRNA precursors for non-coding RNA research\u003cbr\u003eSynthesis of RNA standard template\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/6141.pdf\"\u003eTakara IVTpro mRNA Synthesis System Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/Linearized%20Template%20Vector%20%28linear%29.gb\"\u003eLinearized Template Vector (linear) Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/Positive%20Control%20Template%20%28Fluc%29.gb\"\u003ePositive Control Template (Fluc) Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 Set","offer_id":45986007777359,"sku":"6131","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/6131-Takara-IVTpro-mRNA-Synthesis-System-low-dsRNA-product-600.jpg?v=1782801669"},{"product_id":"cloning-kit-for-mrna-template-bspq-i-6133","title":"Cloning Kit for mRNA Template (BspQ I)","description":"\u003cp\u003eCloning Kit for mRNA Template (BspQ I) 10 rxns\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"10 Rxns","offer_id":45986007744591,"sku":"6133","price":0.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"takara-ivtpro-t7-mrna-synthesis-kit-low-dsrna-6134","title":"Takara IVTpro™ T7 mRNA Synthesis Kit (low dsRNA)","description":"\u003cp\u003eTakara IVTpro T7 mRNA Synthesis Kit (low dsRNA) 20 rxns\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"20 Rxns","offer_id":45986007875663,"sku":"6134","price":0.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"dna-fragmentation-kit-6137","title":"DNA Fragmentation Kit","description":"\u003cp\u003eThis kit is designed to perform random fragmentation of genomic DNAs and other long-chain dsDNAs by enzyme treatments without any special apparatus such as a sonicator, and then to blunt the obtained DNA fragment. Blunt-end fragments may be inserted into blunt-end vectors. The reaction may be stopped after fragmentation is complete if no blunting is required. This kit may also be used to perform the pretreatment in methylated DNA enrichment and high-speed DNA sequence analysis.\u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eGenomic DNA fragmentation dsDNA fragmentation\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/6137\/6137.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD1102\/SD1102-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/6137\/6137_e.v1210Da_1803.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 Rxns","offer_id":45986007908431,"sku":"6137","price":1489.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/6137-DNA-Fragmentation-Kit-product-600.jpg?v=1782801674"},{"product_id":"takara-ivtpro-mrna-synthesis-system-6141","title":"Takara IVTpro™ mRNA Synthesis System","description":"\u003cp\u003eTakara IVTpro mRNA Synthesis System includes all\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStreamlined workflow—complete in vitro transcription workflow, from template preparation to mRNA purification, with a single kit\u003c\/li\u003e\n\u003cli\u003eUltra-high yield—generate up to 200 µg of mRNA per reaction with a cap structure and poly(A) sequence\u003c\/li\u003e\n\u003cli\u003eFlexibility—incorporate a wide variety of capping systems, including CleanCap or ARCA and\/or a modified nucleoside such as pseudouridine\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eConstruction of plasmid DNA for in vitro transcription template\u003cbr\u003eCapped mRNA synthesis using TriLink's CleanCap Reagent AG, or other cap analogs such as Anti-Reverse Cap Analog (ARCA)*\u003cbr\u003eSynthesis of long non-coding RNA or siRNA precursors for non-coding RNA research\u003cbr\u003eSynthesis of RNA standard template\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/6141\/6141_UM.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/6141.pdf\"\u003eTakara IVTpro mRNA Synthesis System Components List\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/Linearized%20Template%20Vector%20%28linear%29.gb\"\u003eLinearized Template Vector (linear) Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Vector%20Documents\/Positive%20Control%20Template%20%28Fluc%29.gb\"\u003ePositive Control Template (Fluc) Vector Sequence in GenBank Format\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"1 Set","offer_id":45986008039503,"sku":"6141","price":1880.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/6141-Takara-IVTpro-mRNA-Synthesis-System-product-600.jpg?v=1782801680"},{"product_id":"cloning-kit-for-mrna-template-6143","title":"Cloning Kit for mRNA Template","description":"\u003cp\u003eCloning Kit for mRNA Template 10rxns\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"10 Rxns","offer_id":45986008137807,"sku":"6143","price":980.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"takara-ivtpro-t7-mrna-synthesis-kit-6144","title":"Takara IVTpro™ T7 mRNA Synthesis Kit","description":"\u003cp\u003eTakara IVTpro T7 mRNA Synthesis Kit 20rxns\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"20 Rxns","offer_id":45986008170575,"sku":"6144","price":1114.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"template-vector-bspq-i-for-t7-mrna-synthesis-6146","title":"Template Vector (BspQ I) for T7 mRNA Synthesis","description":"\u003cp\u003eTemplate Vector (BspQ I) for T7 mRNA Synthesis 10 µl\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"10 µl","offer_id":45986008268879,"sku":"6146","price":419.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"rnase-free-water-9012","title":"RNase-Free Water","description":"\u003cp\u003eThis RNase-Free Water is suitable for use with RNA samples. It is not prepared using hazardous DEPC (diethylpyrocarbonate), and therefore avoids risk of inhibiting RT-PCR which occur with inhibiting residual DEPC.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/9012\/9012_AM61009N_y_COA.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/SD1309\/SD1309-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/9012\/9012_DS.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 mL","offer_id":45986013053007,"sku":"9012","price":249.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/9012-RNase-free-Water-product-600.jpg?v=1782801835"},{"product_id":"smarter-race-5-3-kit-634859","title":"SMARTer® RACE 5’\/3’ Kit","description":"\u003cp\u003eThe SMARTer RACE 5'\/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (Switching Mechanism At 5' End of RNA Template) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. Our carefully designed, specially-modified SMARTer Oligo preferentially captures the 5' ends of the cDNA during cDNA synthesis. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences, thus increasing the likelihood you will amplify the entire sequence of your gene.RACE PCR products are amplified with the provided SeqAmp DNA Polymerase, and cloned into the linearized pRACE vector with In-Fusion HD Cloning. The SMARTer RACE 5'\/3' Kit has been improved to accommodate larger RNA input volumes and perform better on challenging targets than the original SMARTer RACE cDNA Amplification Kit. The In-Fusion HD Cloning Kit, NucleoSpin Gel and PCR Clean-Up Kit, and Stellar Competent Cells are included for your convenience in cloning RACE products. Gene specific RACE primers are supplied by the user.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAdvantage RT-PCR kits\u003c\/li\u003e\n\u003cli\u003eLibrary construction kits\u003c\/li\u003e\n\u003cli\u003eOne-step RT-PCR\u003c\/li\u003e\n\u003cli\u003ePrimeScript Double Strand cDNA Synthesis Kit\u003c\/li\u003e\n\u003cli\u003ePrimeScript II 1st Strand cDNA Synthesis Kit\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/S0310\/S0310-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634913-051115.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634928-073013.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634858-634859.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 Rxns","offer_id":45986059616335,"sku":"634859","price":8528.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/634859-SMARTer-RACE-5-prime-3-prime-Kit-product-600.jpg?v=1782803029"},{"product_id":"smarter-race-5-3-kit-634858","title":"SMARTer® RACE 5’\/3’ Kit","description":"\u003cp\u003eThe SMARTer RACE 5'\/3' Kit allows the synthesis of first-strand cDNA from poly A+ or total RNA via SMART (Switching Mechanism At 5' End of RNA Template) technology, and facilitates the performance of 5'- and 3'-RACE (Rapid Amplification of cDNA Ends) PCR with the kit's Universal Primer Mix. Our carefully designed, specially-modified SMARTer Oligo preferentially captures the 5' ends of the cDNA during cDNA synthesis. Using this SMARTer Oligo, our procedure enriches cDNA pools for 5' sequences, thus increasing the likelihood you will amplify the entire sequence of your gene.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAdvantage RT-PCR kits\u003c\/li\u003e\n\u003cli\u003eLibrary construction kits\u003c\/li\u003e\n\u003cli\u003eOne-step RT-PCR\u003c\/li\u003e\n\u003cli\u003ePrimeScript Double Strand cDNA Synthesis Kit\u003c\/li\u003e\n\u003cli\u003ePrimeScript II 1st Strand cDNA Synthesis Kit\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/S0310\/S0310-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634913-051115.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634928-073013.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634858-634859.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"10 Rxns","offer_id":45986059649103,"sku":"634858","price":5032.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/634858-SMARTer-RACE-5-prime-3-prime-Kit-product-600.jpg?v=1782803030"},{"product_id":"marathon-cdna-amplification-kit-634913","title":"Marathon® cDNA Amplification Kit","description":"\u003cp\u003eThis kit contains reagents for making adaptor-ligated ds cDNA from poly A+ RNA and performing 5' and 3' Marathon RACE PCR using gene-specific primers and a mixture of thermostable DNA polymerases supplied by the user. This kit contains sufficient reagents for 5 cDNA synthesis reactions and 100 PCR reactions.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAdvantage RT-PCR kits\u003c\/li\u003e\n\u003cli\u003eLibrary construction kits\u003c\/li\u003e\n\u003cli\u003eOne-step RT-PCR\u003c\/li\u003e\n\u003cli\u003ePrimeScript Double Strand cDNA Synthesis Kit\u003c\/li\u003e\n\u003cli\u003ePrimeScript II 1st Strand cDNA Synthesis Kit\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/S0310\/S0310-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634913-051115.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634928-073013.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634858-634859.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"100 Rxns","offer_id":45986060173391,"sku":"634913","price":6399.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/634913-Marathon-cDNA-Amplification-Kit-product-600.jpg?v=1782803047"},{"product_id":"smarter-pcr-cdna-synthesis-kit-634926","title":"SMARTer® PCR cDNA Synthesis Kit","description":"\u003cp\u003eA kit for performing twenty cDNA syntheses from nanogram amounts of total or poly A+ RNA. The kit utilizes our SMART (Switching Mechanism At 5' end of RNA Template) technology coupled with PCR amplification to generate high yields of full-length double-stranded cDNA suitable for various applications, including Clontech PCR-Select cDNA subtraction, nondirectional cloning, and preparation of complex cDNA probes. The SMARTer PCR cDNA Synthesis Kit includes the same\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAdvantage RT-PCR kits\u003c\/li\u003e\n\u003cli\u003eLibrary construction kits\u003c\/li\u003e\n\u003cli\u003eOne-step RT-PCR\u003c\/li\u003e\n\u003cli\u003ePrimeScript Double Strand cDNA Synthesis Kit\u003c\/li\u003e\n\u003cli\u003ePrimeScript II 1st Strand cDNA Synthesis Kit\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/634926\/634926.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/S0310\/S0310-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634913-051115.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634928-073013.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Components%20List\/634858-634859.pdf\"\u003eComponents\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"20 Rxns","offer_id":45986061877327,"sku":"634926","price":8390.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/634926-SMARTer-PCR-cDNA-Synthesis-Kit-product-600.jpg?v=1782803053"},{"product_id":"uni-link-aminomodifier-635703","title":"Uni-Link™ AminoModifier","description":"\u003cp\u003eUni-Link AminoModifier is a cyanoethyl phosphoramidite that allows you to directly incorporate a primary amine onto the 5' end of an oligonucleotide using automated synthesis. Label-ON reagents provide an efficient way to directly label oligonucleotides with various reporter molecules. All Label-On Reagents possess the patented 2-aminobutyl-1,3-propanediol (ABPD) backbone, which offer distinct benefits.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMultiple labels can be incorporated because phosphoramidites do not truncate chain elongation at 5' termini.\u003c\/li\u003e\n\u003cli\u003eNatural 3-carbon internucleotide phosphodiester distance is maintained, providing better hybridization and annealing properties than those obtained with other labeling methods.\u003c\/li\u003e\n\u003cli\u003eAttached label is separated from the phosphodiester connection point to reduce steric hindrance. The ABPD backbone has a 7-atom spacer arm that separates the label from the connection point.\u003c\/li\u003e\n\u003cli\u003eLabel incorporation can be easily measured by standard trityl cation assays because phosphoramidites possess a dimethoxytrityl (DMT)-protected hydroxyl group.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eOligo labeling (reagents)\u003cbr\u003eNucleic acid modifications\u003cbr\u003eNucleic acid chemistry (reagents)\u003cbr\u003eNucleic acid labeling (reagents)\u003cbr\u003eNucleic acid synthesis (reagents)\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/635703\/635703-LOT1107009.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/635703\/635703-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"100 mg","offer_id":45986068693071,"sku":"635703","price":2220.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/635703-Uni-Link-AminoModifier-product-600.jpg?v=1782803323"},{"product_id":"chroma-spin-te-100-columns-636073","title":"CHROMA SPIN™+TE-100 Columns","description":"\u003cp\u003eCHROMASPIN+TE-100 COLUMNS X50\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"50 Each","offer_id":45986073247823,"sku":"636073","price":3855.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"chroma-spin-te-400-columns-636076","title":"CHROMA SPIN™+TE-400 Columns","description":"\u003cp\u003eCHROMASPIN+TE-400 COLUMNS X50\u003c\/p\u003e","brand":"Takara Bio","offers":[{"title":"50 Each","offer_id":45986073444431,"sku":"636076","price":3855.0,"currency_code":"ILS","in_stock":true}]},{"product_id":"chroma-spin-te-1000-columns-636079","title":"CHROMA SPIN™+TE-1000 Columns","description":"\u003cp\u003eCHROMA SPIN+TE-1000 Columns are used for the purification of nucleic acids larger than 1,000 bp in size. Matrix is in TE buffer (10 mM Tris-HCl [pH 8.0], and 1 mM EDTA).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTE buffer: ideal for routine DNA applications\u003c\/li\u003e\n\u003cli\u003eSTE (0.1 M NaCl + TE) buffer: used for applications requiring a higher salt concentration to prevent ionic interaction between molecules\u003c\/li\u003e\n\u003cli\u003eDEPC-treated water (+ 0.1 mM EDTA): useful for RNA purification and applications requiring a completely nuclease-free environment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/636079\/636079.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/S0146\/S0146-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/User%20Manual\/CHROMA%20SPIN%20Columns%20User%20Manual\/CHROMA%20SPIN%20Columns%20User%20Manual.pdf\"\u003eUser Manual (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Takara Bio","offers":[{"title":"50 Each","offer_id":45986073641039,"sku":"636079","price":3962.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/636079-CHROMA-SPINplusTE-1000-Columns-product-600.jpg?v=1782803351"},{"product_id":"chroma-spin-1000-depc-h2o-columns-636093","title":"CHROMA SPIN™-1000+DEPC-H2O Columns","description":"\u003cp\u003eCHROMA SPIN-1,000+DEPC-H2O Columns are used for the purification of nucleic acids larger than 1,000 bp in size. Matrix is in DEPC-treated, autoclaved H2O + 0.1 mM EDTA (pH 8.0).\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTE buffer: ideal for routine DNA applications\u003c\/li\u003e\n\u003cli\u003eSTE (0.1 M NaCl + TE) buffer: used for applications requiring a higher salt concentration to prevent ionic interaction between molecules\u003c\/li\u003e\n\u003cli\u003eDEPC-treated water (+ 0.1 mM EDTA): useful for RNA purification and applications requiring a completely nuclease-free environment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/Certificate%20of%20Analysis\/636093\/636093.pdf\"\u003eCertificate of Analysis (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.takarabio.com\/documents\/SDS\/S0146\/S0146-EGHS-EN.pdf\"\u003eSafety Data Sheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca 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