{"title":"Protein Research","description":null,"products":[{"product_id":"kta-pure-chromatography-system-29018226","title":"ÄKTA pure™ chromatography system","description":"\u003cp\u003eThe ÄKTA pure™ chromatography system is a versatile and modular system designed for reliable purification of biomolecules, including proteins, peptides and nucleic acids. Major features:\u003c\/p\u003e\n\u003cp\u003e• Modular system design: Supports a wide range of alternatives for any type of purification processes from basic to advanced automated applications\n• UNICORN™ control software: Facilitates intuitive method creation, system control, and evaluation\n• Reliable system: With components and integrated features based on the proven design of ÄKTA protein purification systems\n• Predefined method settings: For all our laboratory-scale chromatography columns\n• Practical size: Easy placement on laboratory bench or in cold cabinet\u003c\/p\u003e\n\u003cp\u003eChoose the ÄKTA pure system that fits your purification journey\u003c\/p\u003e\n\u003cp\u003eÄKTA pure™ 25: Ideal for a broad range of research applications and purification tasks in multiuser labs.\u003c\/p\u003e\n\u003cp\u003eÄKTA pure™ 150: Supports higher sample loads and throughput, helping you maximize productivity and streamline routine large-scale purifications.\u003c\/p\u003e\n\u003cp\u003eÄKTA pure™ T: With 2 fixed wavelengths at 260 and 280 nm ÄKTA pure™ T offers a way of addressing complex purification needs. Available in 25 mL\/min and 150 mL\/min formats, it retains the flexibility and additional module selection options as ÄKTA pure™ L and M. If you have an existing ÄKTA pure™ L chromatography system, it can be upgraded with the U9-T UV monitor to perform a broader set of applications.\u003c\/p\u003e\n\u003cp\u003eÄKTA pure ™ micro: Offers enhanced resolution and sharper peaks is ideal for supporting cryo-EM applications. Choose up to 3 wavelengths from 190 to 700 nm.\u003c\/p\u003e\n\u003cp\u003eA wide range of optional modules are available to customize the system according to specific purification needs. Use the Purify App tool build and visualize any ÄKTA pure™ system\u003c\/p\u003e\n\u003cp\u003eUNICORN™ control software is based on an integrated controller and an intuitive computer-based interface. Key benefits include:\u003c\/p\u003e\n\u003cp\u003e• Simple method creation: Drag-and-drop operations for creating chromatography methods.\n• Advanced method creation: Text instructions gives you the freedom to customize chromatography methods\n• Modular features: Includes column logbook and design of experiments (DoE) functionality.\n• Licensing options: Remote access for creating methods, evaluating results and purification monitoring.\u003c\/p\u003e\n\u003cp\u003eLearn more on the UNICORN™ software page\u003c\/p\u003e\n\u003cp\u003eVarious prepacked columns are available for different scales of purification, with options to pack your own columns with loose resins using the ÄKTA pure.\u003c\/p\u003e\n\u003cp\u003eFind the best resin or column for application and molecule type with the Purify App\u003c\/p\u003e\n\u003cp\u003eRegardless of the configuration, the ÄKTA pure system package includes:\u003c\/p\u003e\n\u003cp\u003e• Two bio-inert high-performance system pumps\n• System pressure monitor for column protection\n• Mixer for gradient mixing\n• Injection valve for sample application\n• UV monitor for biomolecule detection\n• Conductivity monitor for gradient verification\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eModular system design: Supports a wide range of alternatives for any type of purification processes from basic to advanced automated applications\u003c\/li\u003e\n\u003cli\u003eUNICORN™ control software: Facilitates intuitive method creation, system control, and evaluation\u003c\/li\u003e\n\u003cli\u003eReliable system: With components and integrated features based on the proven design of ÄKTA protein purification systems\u003c\/li\u003e\n\u003cli\u003ePredefined method settings: For all our laboratory-scale chromatography columns\u003c\/li\u003e\n\u003cli\u003ePractical size: Easy placement on laboratory bench or in cold cabinet\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eÄKTA pure 25 L, ÄKTA pure 25 M, ÄKTA pure™ 25 T, ÄKTA pure™ 150 T: Flexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale.\u003c\/li\u003e\n\u003cli\u003eÄKTA pure 25 L, ÄKTA pure 25 M, ÄKTA pure™ micro: Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T.\u003c\/li\u003e\n\u003cli\u003eÄKTA pure 150 L, ÄKTA pure™ 150 M: Tailored and reliable chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to tens of gram levels of target product.\u003c\/li\u003e\n\u003cli\u003eÄKTA pure 150 L, ÄKTA pure™ 150 M, ÄKTA pure™ 25 T, ÄKTA pure™ 150 T: Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R.\u003c\/li\u003e\n\u003cli\u003eÄKTA pure™ micro: Chromatography system for high-resolution purification in microliter scale.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eÄKTA pure 25 L (29018224)\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow Rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e25 mL\/min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePressure range (system pump)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 20 MPa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWavelength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFixed at 280 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFlexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication supported\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAffinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAutomatic buffer preparation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAutosampler injection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo (optional)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBuffer selection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUpgradeable to 14 inlets\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCompliance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDepth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e470 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow cell path length\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 mm (optional 5 mm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.001 to 25 mL\/min (normal range), 0.01 to 50 mL\/min (column packing flow)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHeight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e629 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMultiple sample injection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUpgradeable to 5 samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMultiple wavelength detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFixed wavelength\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMultistep purification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eYes, (optional)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOperating pressure (sample pump) max.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOperating pressure max.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 MPa (2900 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePower consumption\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e300 VA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtection class\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIP 21\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePump type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePiston pump, metering type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRelative humidity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% to 95% (noncondensing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSafety certifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCE, ETL, UKCA, EAC, KC, RCM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTubing i.d. (flow path)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.5 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUV wavelength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e280 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eViscosity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.35 to 10.0 cP (system pump)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eVoltage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 to 120\/220 to 240 VAC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWeight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUp to 53 kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWidth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e535 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH monitoring\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 14\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability, cleaning-in-place (CIP)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 14\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability, operational\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 to 12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eÄKTA pure 25 M (29018226)\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow Rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e25 mL\/min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePressure range (system pump)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 20 MPa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWavelength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMulti – choose up to 3 from 190nm to 700nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFlexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication supported\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAffinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAutomatic buffer preparation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAutosampler injection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo (optional)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBuffer selection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUpgradeable to 14 inlets\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCompliance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDepth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e470 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow cell path length\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 mm (optional 0.5 mm and 10 mm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.001 to 25 mL\/min (normal range), 0.01 to 50 mL\/min (column packing flow)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHeight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e629 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMultiple sample injection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUpgradeable to 5 samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMultiple wavelength detection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUp to 3 wavelengths\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMultistep purification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eYes, (optional)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOperating pressure (sample pump) max.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOperating pressure max.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 MPa (2900 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePower consumption\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e300 VA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtection class\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIP 21\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePump type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePiston pump, metering type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRelative humidity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% to 95% (noncondensing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSafety certifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCE, ETL, UKCA, EAC, KC, RCM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTubing i.d. (flow path)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.5 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eUV wavelength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e190 to 700 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eViscosity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.35 to 10.0 cP (system pump)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eVoltage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 to 120\/220 to 240 VAC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWeight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUp to 53 kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWidth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e535 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH monitoring\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 14 (optional)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability, cleaning-in-place (CIP)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 14\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability, operational\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 to 12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eÄKTA pure™ 25 T (29707636)\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow Rate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e25 mL\/min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePressure range (system pump)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 20 MPa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWavelength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDual 260\/280 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFlexible and intuitive chromatography system for fast purification of proteins and antibodies from microgram to gram scale.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eApplication supported\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAffinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAutomatic buffer preparation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAutosampler injection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCompliance\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eThe product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDepth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e470 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHeight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e629 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMultiple sample injection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUpgradeable to 5 samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMultistep purification\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eYes, (optional)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOperating pressure max.\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20 MPa (2900 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePower consumption\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e300 VA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProtection class\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIP 21\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePump type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePiston pump, metering type\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eRelative humidity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e20% to 95% (noncondensing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSafety certifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2006\/42\/EC (MD), 2004\/108\/EC (EMC), 1999\/5\/EC (R\u0026amp;TTE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTubing i.d. (flow path)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.5 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eViscosity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0.35 to 10.0 cP (system pump)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eVoltage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 to 120\/220 to 240 VAC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWeight\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eUp to 53 kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eWidth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e535 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH monitoring\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 14 (optional)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability, cleaning-in-place (CIP)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e0 to 14\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003epH stability, operational\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 to 12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-29502-pdf\" target=\"_blank\" rel=\"noopener\"\u003eData file: ALIAS Bio autosampler for ÄKTA pure and ÄKTA avant\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-16276-pdf\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA pure™ chromatography systems\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/_UN7x62W9U2j0JId8NsGbw-pdf\" target=\"_blank\" rel=\"noopener\"\u003eUNICORN™ 7 software\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17730-pdf\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA lab-scale systems: Overview and comparison\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-23665-pdf\" target=\"_blank\" rel=\"noopener\"\u003eHistidine-tagged protein purification and detection brochure\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-29175-pdf\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA™ avant and ÄKTA™ pure preventive maintenance heet\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17327-pdf\" target=\"_blank\" rel=\"noopener\"\u003eTraining and applications support for ÄKTA protein purification systems for research and process development\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17578-pdf\" target=\"_blank\" rel=\"noopener\"\u003ePrepacked columns for ÄKTA™ systems: Selection guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-16297-pdf\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA pure Unpacking Instruction\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-29488-pdf\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA pure - Guide for connecting Light Scattering\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-17267-pdf\" target=\"_blank\" rel=\"noopener\"\u003eGood ÄKTA system practice Cue Cards\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29744403.zip\" target=\"_blank\" rel=\"noopener\"\u003eRTU Win 10 Image SP1 Software, release notes, and installation instructions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29740748.zip\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA pure 25 Instrument Configuration software v2.4.0.8, Release notes and installation instructions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29740747.zip\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA pure 150 Instrument Configuration software v2.3.0.7, release notes, and installation instructions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.cytivalifesciences.com\/api\/public\/content\/digi-33828-pdf\" target=\"_blank\" rel=\"noopener\"\u003eReal-Time Unit Software Release notes and installation instructions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29414475.pdf\" target=\"_blank\" rel=\"noopener\"\u003eUNICORN Quick Installation Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29325017.pdf\" target=\"_blank\" rel=\"noopener\"\u003eInstall Injection Valve V9-J and V9M-J Instructions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/28968650.pdf\" target=\"_blank\" rel=\"noopener\"\u003eInstall optional outlet valves Instructions for Use\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29021461.pdf\" target=\"_blank\" rel=\"noopener\"\u003eInstall Conductivity Monitor C9n and C9M Instructions\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/28968652.pdf\" target=\"_blank\" rel=\"noopener\"\u003eInstall Air Sensor L9\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29020658.pdf\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA pure 25 Product Documentation\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/28969192.pdf\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA avant, ÄKTA pcc, and ÄKTA pure Instructions for Use\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29142436.pdf\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA Systems Column protection\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29503110.pdf\" target=\"_blank\" rel=\"noopener\"\u003eUNICORN 7.6 Command-line Installation Guide\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/d3.cytivalifesciences.com\/prod\/IFU\/29503111.pdf\" target=\"_blank\" rel=\"noopener\"\u003eUNICORN 7.6 Installation Checklist\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/bioprocess-modular-system-for-liquid-chromatography-p-11559\" target=\"_blank\" rel=\"noopener\"\u003eBioProcess Modular System for liquid chromatography\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/sample-inlet-valve-kit-v9-is-p-04826\" target=\"_blank\" rel=\"noopener\"\u003eSample Inlet Valve Kit V9-IS\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/air-sensors-p-06273\" target=\"_blank\" rel=\"noopener\"\u003eAir Sensors for ÄKTA pilot 600™\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/akta-avant-p-06264\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA™ avant chromatography system\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/superdex-peptide-32-300-p-05802\" target=\"_blank\" rel=\"noopener\"\u003eSuperdex Peptide 3.2\/300\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/aktapilot-system-p-05845\" target=\"_blank\" rel=\"noopener\"\u003eÄKTApilot™ system\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/akta-pcc-and-bioprocess-pcc-continuous-chromatography-systems-p-06265\" target=\"_blank\" rel=\"noopener\"\u003eÄKTA™ pcc and BioProcess pcc continuous chromatography systems\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/aktaxpress-p-06166\" target=\"_blank\" rel=\"noopener\"\u003eÄKTAxpress\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/aktaprime-plus-p-05771\" target=\"_blank\" rel=\"noopener\"\u003eÄKTAprime plus\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.cytivalifesciences.com\/en\/us\/products\/items\/injection-valve-v9-inj-p-05180\" target=\"_blank\" rel=\"noopener\"\u003eInjection Valve, V9-Inj\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Cytiva","offers":[{"title":"25 mL\/min \/ 0 to 20 MPa \/ Multi – choose up to 3 from 190nm to 700nm (29018226)","offer_id":45982352244815,"sku":"29018226","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"25 mL\/min \/ 0 to 20 MPa \/ Multi – choose up to 3 from 190nm to 700nm (29302479)","offer_id":45982352277583,"sku":"29302479","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"25 mL\/min \/ 0 to 20 MPa \/ Dual 260\/280 nm","offer_id":45982352310351,"sku":"29707636","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"25 mL\/min \/ 0 to 20 MPa \/ Fixed at 280 nm","offer_id":45982352343119,"sku":"29018224","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"150 mL\/min \/ 0 to 5 MPa \/ Multi – choose up to 3 from 190nm to 700nm","offer_id":45982352375887,"sku":"29046694","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"150 mL\/min \/ 0 to 5 MPa \/ Dual 260\/280 nm","offer_id":45982352408655,"sku":"29707638","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"150 mL\/min \/ 0 to 5 MPa \/ Fixed at 280 nm","offer_id":45982352441423,"sku":"29046665","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/tjh2RRYIRfiZhHgAmE7nSA-original-png_268b856f-d8e7-4f0b-9703-af76b423eab3.png?v=1788947758"},{"product_id":"wyatt-dawn-multi-angle-light-scattering-detector","title":"DAWN Multi-Angle Light Scattering Detector","description":"\u003cspan\u003e\u003cp align=\"center\"\u003e\u003cimg fetchpriority=\"high\" src=\"https:\/\/www.wyatt.com\/wp-content\/uploads\/2019\/10\/DAWN-Hero-Neon-9-19.jpg\" width=\"55%\"\u003e\u003c\/p\u003e\u003c\/span\u003e\n\u003cbr\u003e\n\u003cp\u003eThe world's most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.\u003c\/p\u003e\n\u003cp\u003ePremier MALS\u003c\/p\u003e\n\u003cp\u003eThe DAWN instrument is the premier Multi-Angle static Light Scattering (MALS) detector for absolute characterization of the molar mass and size of macromolecules and nanoparticles in solution. It offers the highest sensitivity, the widest range of molecular weight, size, and concentration, and the largest selection of configurations and optional modules for enhanced capabilities.\u003c\/p\u003e\n\u003chr class=\"no_line\" style=\"margin: 0 auto 10px auto\"\u003e\n\u003cp\u003eSeparation First\u003c\/p\u003e\n\u003cp\u003eMALS is most often used in conjunction with a separation system such as Gel Permeation Chromatography (GPC-MALS), Size Exclusion Chromatography (SEC-MALS), or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size, and composition, independent of column calibration by reference standards. \u003c\/p\u003e\n\u003chr class=\"no_line\" style=\"margin: 0 auto 10px auto\"\u003e\n\u003cp\u003eSupported by ASTRA\u003csup\u003e™\u003c\/sup\u003e Software\u003c\/p\u003e\nASTRA Software and its built-in analysis modules support the DAWN MALS instrument, providing the most versatile analysis available for online multi-angle and dynamic light scattering measurements. \n\u003cp\u003eOverview\u003c\/p\u003e\n\u003cp\u003eThe DAWN and its companion \u003ca href=\"https:\/\/www.wyatt.com\/products\/instruments\/optilab-refractive-index-detector.html\"\u003eOptilab\u003csup\u003e™\u003c\/sup\u003e\u003c\/a\u003e dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.\u003c\/p\u003e\n\u003cp\u003eSEC-MALS with a DAWN and Optilab characterizes conformation and branching of \u003cb\u003esynthetic and natural polymers\u003c\/b\u003e larger than 10 nm in rms radius \u003ci\u003eR\u003c\/i\u003e\u003csub\u003eg\u003c\/sub\u003e. The addition of a \u003ca href=\"https:\/\/www.wyatt.com\/products\/instruments\/viscostar-online-differential-viscometer.html\"\u003eViscoStar\u003csup\u003e™\u003c\/sup\u003e\u003c\/a\u003e differential viscometer creates a GPC triple-detection system that can analyze molecular conformation and size below the \u003ci\u003eR\u003c\/i\u003e\u003csub\u003eg\u003c\/sub\u003e limit of 10 nm and determine Mark-Houwink Sakurada coefficients.\u003c\/p\u003e\n\u003cp\u003eThe combination of a UV\/Vis absorbance detector with a DAWN instrument and Optilab refractive index detector creates a UV-MALS-RI triple-detection system, for analysis of copolymers and protein conjugates (glycoproteins, PEGylated proteins, surfactant-bound membrane proteins, etc.). With the addition of a \u003ca href=\"https:\/\/store.wyatt.com\/shop\/udawn\/udawn3\/wiq\/\"\u003eWyattQELS\u003csup\u003e™\u003c\/sup\u003e DLS module\u003c\/a\u003e for size determination, protein conformation may be analyzed.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ci\u003eM\u003c\/i\u003e\u003csub\u003ew\u003c\/sub\u003e from 200 Da to 1000 MDa\u003c\/li\u003e\n\u003cli\u003eSensitivity: 10 ng\/mL 100 kDa polystyrene in THF, 0.4 µg\/mL BSA (66.4 kDa) in PBS\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ci\u003eR\u003c\/i\u003e\u003csub\u003eg\u003c\/sub\u003e from 10 to 500 nm, up to 1000 nm with shape-specific models\u003c\/li\u003e\n\u003cli\u003eUltra-stable, high-power laser\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe DAWN may also be used in batch (off-line) mode to characterize the weight-average molar mass, z-average size \n  and second virial coefficient of unfractionated samples.\u003c\/p\u003e\n\u003cp\u003eIn conjunction with a \n  \u003ca href=\"https:\/\/www.wyatt.com\/products\/instruments\/calypso-ii-label-free-biomolecular-interactions.html\"\u003eCalypso\u003csup\u003e™\u003c\/sup\u003e   CG-MALS\u003c\/a\u003e system, the DAWN characterizes macromolecular interactions, label-free and immobilization-free: affinity and absolute stoichiometry of self- and hetero-association of simple or complex molecular systems. The Calypso system also automates the determination of second virial coefficients, cross virial coefficients, average molar masses and sizes.\u003c\/p\u003e\n\u003cp\u003eUltrasonic Cleaning Included\u003c\/p\u003e\n\u003cp\u003eEach DAWN instrument includes an embedded \u003ca href=\"https:\/\/www.wyatt.com\/accessories\/comet.html\"\u003e\u003cb\u003eCOMET\u003csup\u003e™\u003c\/sup\u003e\u003c\/b\u003e\u003c\/a\u003e ultrasonic transducer that cleans the flow cell automatically by agitating the fluid and loosening particles from the glass surfaces. \u003c\/p\u003e\n\u003cp\u003eTemperature-Controlled Models\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eAmbient model\u003c\/li\u003e\n\u003cli\u003eUltra-High-Temperature (UHT) model, from ambient up to +210 °C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eStandard Peltier heated\/cooled (H\/C) model, temperature control from -15 °C to +150 °C\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMolar Mass Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e200 Da to 1 GDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMolecular Size Range ( R g )\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e≈ 10 to 500 nm, up to 1000 nm with shape-specific models\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMolecular Size Range ( R h )\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIn flow mode, 0.5 nm to 300 nm ; batch mode 0.5 nm to 1 μm (requires WyattQELS DLS module or DynaPro NanoStar + fiber optic connection).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSensitivity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10 ng of 100 kDa polystyrene in THF or 200 ng of BSA in PBS, assuming standard 7.8 mm x 300 mm SEC column and no post-column filter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMobile Phase Compatibility\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAll-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica or F2 glass, and Kalrez.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMALS Detectors\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eHigh-gain, high dynamic range photodiodes at 18 detection angles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAuxiliary Detectors\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eLaser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDynamic Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3,300,000:1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDLS Detector (optional)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eWyattQELS dynamic light scattering module installs directly inside the DAWN chassis. Alternatively, the optical fiber pickup of the DynaPro NanoStar cuvette-based DLS instrument or Mobius DLS-ELS instrument may be installed in the DAWN.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLaser Wavelength\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e658 nm ± 4 nm; 785 nm optional for use with fluorescing samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eLaser Power Control\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eProgrammable 10% to 100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFlow Cells\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFused Silica, optimal for solvent refractive index less than 1.50; F2, optimal for solvent refractive index above 1.50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTemperature Options\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAmbient Heated\/Cooled (HC) -15 °C to +150 °C Ultra-High-Temperature (UHT) ambient to +210 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAnalog Inputs\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e4 differential analog inputs with 24 bit resolution. Input range -10 V to +10 V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAnalog Outputs\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e2 analog outputs from user selectable measurements channels -10 V to +10 V\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eOther Inputs\/Outputs\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAlarm in, Alarm out\/retransmit, Auto-Inject in, Auto inject contact closure retransmit, Recycle In\/Out (operates Orbit recycle valve)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eComputer Interface\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEthernet\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFront Panel Display\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10.1\" (257 mm), capacitive high-resolution screen with multi-touch functionality, displays signals, graphs, instrument settings and diagnostics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eDimensions\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e58 cm (L) x 36 cm (W) x 26 cm (H)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.wyatt.com\/products\/dawn-brochure-request.html\" target=\"_blank\"\u003eBrochure\u003c\/a\u003e\u003c\/p\u003e","brand":"Wyatt Technology","offers":[{"title":"Ambient \/ Fused Silica, 658 nm \/ None","offer_id":46488396562511,"sku":"WD3-016500","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica, 658 nm \/ WyattQELS","offer_id":46488408555599,"sku":"WD3-016501","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica, 658 nm \/ DLS Fiber","offer_id":46488408588367,"sku":"WD3-0165DLS","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica, 785 nm \/ None","offer_id":46488408621135,"sku":"WD3-017800","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica, 785 nm \/ WyattQELS","offer_id":46488408653903,"sku":"WD3-017801","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica, 785 nm \/ DLS Fiber","offer_id":46488408686671,"sku":"WD3-0178DLS","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica, Aqueous, 658 nm \/ None","offer_id":46488408719439,"sku":"WD3-01AQ6500","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica, Aqueous, 658 nm \/ WyattQELS","offer_id":46488408752207,"sku":"WD3-01AQ6501","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica, Aqueous, 658 nm \/ DLS Fiber","offer_id":46488408784975,"sku":"WD3-01AQ65DLS","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica + Wide-Bore, 658 nm \/ WyattQELS","offer_id":46488408817743,"sku":"WD3-01036501","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Fused Silica + Wide-Bore, 658 nm \/ DLS Fiber","offer_id":46488408850511,"sku":"WD3-010365DLS","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Wide-Bore Fused Silica, 658 nm \/ WyattQELS","offer_id":46488408883279,"sku":"WD3-036501","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ Wide-Bore Fused Silica, 658 nm \/ DLS Fiber","offer_id":46488408916047,"sku":"WD3-0365DLS","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ 2 × Wide-Bore Fused Silica, 658 nm \/ WyattQELS","offer_id":46488408948815,"sku":"WD3-03036501","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ 2 × Wide-Bore Fused Silica, 658 nm \/ DLS Fiber","offer_id":46488408981583,"sku":"WD3-030365DLS","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ambient \/ To be specified \/ To be specified","offer_id":46488409014351,"sku":"WD3","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Heated\/Cooled \/ Fused Silica, 658 nm \/ None","offer_id":46488396595279,"sku":"WD3HC-016500","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Heated\/Cooled \/ Fused Silica, 658 nm \/ WyattQELS","offer_id":46488409047119,"sku":"WD3HC-016501","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Heated\/Cooled \/ Fused Silica, 658 nm \/ DLS Fiber","offer_id":46488409079887,"sku":"WD3HC-0165DLS","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Heated\/Cooled \/ Fused Silica, Aqueous, 658 nm \/ None","offer_id":46488409112655,"sku":"WD3HC-01AQ6500","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Heated\/Cooled \/ Fused Silica, Aqueous, 658 nm \/ WyattQELS","offer_id":46488409145423,"sku":"WD3HC-01AQ6501","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Heated\/Cooled \/ Fused Silica, Aqueous, 658 nm \/ DLS 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nm \/ WyattQELS","offer_id":46488409342031,"sku":"WD3TR-01AQ6501","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Temperature Regulated \/ Fused Silica, Aqueous, 658 nm \/ DLS Fiber","offer_id":46488409374799,"sku":"WD3TR-01AQ65DLS","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Temperature Regulated \/ To be specified \/ To be specified","offer_id":46488409407567,"sku":"WD3TR","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ultra-High Temperature \/ Fused Silica, 658 nm \/ None","offer_id":46488396660815,"sku":"WD3UH-016500","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Ultra-High Temperature \/ To be specified \/ To be specified","offer_id":46488409440335,"sku":"WD3UH","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/DAWN-Hero-Neon-9-19_ec09b7e7-d1cb-4312-92a5-e60c5d7ceefb.jpg?v=1787675350"},{"product_id":"refeyn-twomp-mass-photometer","title":"Refeyn TwoMP Mass Photometer","description":"\u003cp\u003eMass photometry is a revolutionary new method of analyzing molecules. It enables the accurate mass measurements of single molecules in solution, in their native state and without the need for labels. For molecular mass measurements with unmatched sensitivity, speed and simplicity of use, a TwoMP mass photometer offers wide mass range and single-molecule resolution.\u003c\/p\u003e\n\u003cp\u003eRapid determination of stability by monitoring aggregation and degradation in a single measurement\u003c\/p\u003e\n\u003cp\u003eHigh-fidelity measurements of molecular mass\u003c\/p\u003e\n\u003cp\u003eIntuitive acquisition and data analysis software\u003c\/p\u003e\n\u003cp\u003eEasy setup – a compact, benchtop instrument with minimum installation requirements\u003c\/p\u003e\n\u003cp\u003eWith its high sensitivity, the TwoMP is ideally suited for measurements at physiological (i.e. low) concentrations. The high dynamic range intrinsic to single molecule counting techniques ensures low-abundance species are still captured accurately.\u003c\/p\u003e\n\u003cp\u003eRefeyn’s software automatically controls the acquisition process and performs the mass analysis in minutes. You can intuitively interpret the mass distribution results without the need for any prior knowledge.\u003c\/p\u003e\n\u003cp\u003eA compact, bench-top instrument with minimal installation requirements. Measurements only require a few microliters of sample and clean sample-carrier slides.\u003c\/p\u003e\n\u003cp\u003eTwoMP Auto | Reliable, accurate walkaway solution\u003c\/p\u003e\n\u003cp\u003eAutomated mass photometry combines the ease and efficiency of automation with the unmatched sensitivity and simplicity of Refeyn’s molecular mass measurement technology. The automated mass photometer frees up operator time and offers enhanced precision, enabling rapid measurement of multiple samples with low sample consumption. Automation is compatible with the TwoMP.\u003c\/p\u003e\n\u003cp\u003eRapid, automated measurement of multiple samples\u003c\/p\u003e\n\u003cp\u003eIdeal for screening and titration assays\u003c\/p\u003e\n\u003cp\u003eHighly reproducible data with a user-friendly software\u003c\/p\u003e\n\u003cp\u003eThe TwoMP Auto streamlines your workflow, with highly reproducible data \u0026amp; a user-friendly software\u003c\/p\u003e\n\u003cp\u003e'With the TwoMP Auto, you can run 24 measurements in as little as 90 minutes (or up to 180 minutes, depending on the protocol).\u003c\/p\u003e\n\u003cp\u003eThe TwoMP Auto delivers automated mass photometry\u003c\/p\u003e\n\u003cp\u003eStart where you are today and scale as you grow – both configurations deliver the same trusted mass photometry results.\u003c\/p\u003e\n\u003cp\u003e• Work with antibodies or other proteins, or nucleic acids\n• Require the highest mass resolution available in mass photometry\n• Want to deeply characterize sample purity and homogeneity, aggregation, binding, molecular integrity, stoichiometry and complex formation\n• Require full access to raw data for custom analysis\n• Need publication-ready figures that can be fully customized\n• Want to use application-specific software packages from Refeyn\u003c\/p\u003e\n\u003cp\u003e• Need all the capabilities of TwoMP with automated sample handling for higher throughput\n• Need to screen multiple samples or conditions in a single, unattended session\n• Want significant walkaway time and consistent, reproducible results across samples and operators\u003c\/p\u003e\n\u003cp\u003e• Have a TwoMP mass photometer\n• Work with proteins\n• Need to measure samples at higher concentrations than standard TwoMP allows (up to tens of micromolar)\n• Want to detect and characterize weak or transient interactions\n• Require rapid, minimal-sample analysis with quick experimental setup\u003c\/p\u003e\n\u003cp\u003eProtein-protein interactions: Antigen-antibody interactions are a prime example of molecular systems that can be studied using mass photometry, which can be applied to determine binding affinities for mono- and multivalent interactions.\u003c\/p\u003e\n\u003cp\u003eDNA-protein interactions: Mass photometry can be used to characterise DNA-protein complexes, which are crucial in gene expression, replication and DNA repair. In this example, mass photometry not only allows detection of DNA binding but also provides information on how the oligomeric state of the protein changes upon DNA binding (Figure 2).\u003c\/p\u003e\n\u003cp\u003eProtein oligomerization: Many proteins adopt a particular oligomeric form under certain conditions. In Figure 1, mass photometry characterises four different proteins: protein A, beta-amylase, urease and thyroglobulin. Through this characterisation, these proteins showcase a range of behaviours – from purely monomeric (as in Protein A) to a dynamic equilibrium between multiple states.\u003c\/p\u003e\n\u003cp\u003eComplex formation: Ribosomes are macromolecular assemblies of protein and RNA, and are central sites for protein synthesis. Bacterial ribosomes (in this example from E. coli) are \u0026gt;2 MDa in size. Magnesium ions play a subtle yet important role in the assembly of intact (70S) complexes. In the absence of magnesium, E. coli ribosomes rapidly disassemble into their 30S and 50S subunits. Mass photometry allows us to monitor these processes.\u003c\/p\u003e\n\u003cp\u003eThe liquid-handling robot autonomously runs 24 measurements in *as little as 90 minutes (up to 180 minutes, depending on the protocol).\u003c\/p\u003e\n\u003cp\u003eA straightforward, repeated measurement of a sample containing the antibody IgG illustrates the precision of the automated mass photometer, which is reflected in the high degree of reproducibility of the resulting data (Fig. 1).\u003c\/p\u003e\n\u003cp\u003eFigure 1A shows histograms of 12 repeated measurements of an IgG sample made using automated mass photometry. Across the wells that contain the sample, the measured mass (Fig. 1B) and total counts (Fig. 1C) show low variability.\u003c\/p\u003e\n\u003cp\u003eFigure 1 Automated mass photometry measurements are highly reproducible. Autonomously run mass photometry measurements show (A) the mass distributions of IgG forming monomers and dimers (inset: mass histograms for each well). The measured mass (B) and total counts (C) across the wells show low variability.\u003c\/p\u003e\n\u003cp\u003eQ: What type of biomolecules can I measure with the TwoMP?\nA: The TwoMP has a wide mass range, allowing the study of different biomolecules, including proteins and protein complexes, nucleic acids, and large macromolecular complexes.\u003c\/p\u003e\n\u003cp\u003eQ: What are the optimization steps necessary for a mass photometry measurement?\nA: The only optimization step necessary prior to a mass photometry measurement is to adjust the final concentration of the sample; the sample concentration on the instrument should be between 100 pM and 100 nM. The optimal concentration range for a mass photometry measurement is 5-20 nM.\u003c\/p\u003e\n\u003cp\u003eQ: Does the shape\/conformation of my protein affect the mass measured?\nA: For biomolecules within the mass detection range of the TwoMP (30 kDa to 5 MDa), the shape\/conformation doesn’t influence the interferometric contrast and thus mass measured by the mass photometer.\u003c\/p\u003e\n\u003cp\u003eQ: If my protein of interest is fluorescent under the laser light, will it affect the mass measured?\nA: In mass photometry, the mass measurement depends on the interference between the light scattered by the molecule and reflected light from the glass surface. This interference signal will be orders of magnitude greater than the fluorescence signal, so the mass measurement will not be affected.\u003c\/p\u003e\n\u003cp\u003eQ: Which mass photometers are compatible with the ‘Auto’?\nA: If you already have a TwoMP, and SamuxMP, these mass photometers can be upgraded to automated systems. New automated mass photometers are available for purchase as TwoMP Auto and SamuxMP Auto.\u003c\/p\u003e\n\u003cp\u003eQ: What consumables are available for the TwoMP Auto?\nA: We recommend using the Refeyn mass photometry consumables for Automated Systems. If you have a new TwoMP Auto instrument, you can order a Starter Kit, which includes everything you need to prepare samples and run a measurement (sample carrier slides and sample well cassettes to support 120 sample measurements, and the Alignment Assist Package to affix the sample well cassette). You can top up your supply of consumables with the Sample Preparation Package, which includes ready-to-use sample carrier slides and sample well cassettes to support 300 sample measurements or the Sample Carrier Slide-Only Package for Automated Systems (based on your needs).\u003c\/p\u003e\n\u003cp\u003eQ: Is the TwoMP Auto able to dilute samples?\nA: Yes, there is an ‘in-plate’ dilution capability, and the user can select the following options in the software (version R1 2023): 1\/2, 1\/4, 1\/8, 1\/10, 1\/25, 1\/50, 1\/100, 1\/250, 1\/500, 1\/1000.\u003c\/p\u003e\n\u003cp\u003eQ: What are the specs for TwoMP Auto?\nA: In terms of mass photometry, these are the same as the mass photometer inside the ‘Auto’. For ‘Auto’ specific specs, please refer to the data sheet.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eHigh-fidelity measurements of molecular mass\u003c\/li\u003e\n\u003cli\u003eMinimal quantities of sample required\u003c\/li\u003e\n\u003cli\u003eIntuitive acquisition and data analysis software\u003c\/li\u003e\n\u003cli\u003eEasy setup – a compact, benchtop instrument with minimum installation requirements\u003c\/li\u003e\n\u003cli\u003eHigh-fidelity measurements: With its high sensitivity, the TwoMP is ideally suited for measurements at physiological (i.e. low) concentrations. The high dynamic range intrinsic to single molecule counting techniques ensures low-abundance species are still captured accurately.\u003c\/li\u003e\n\u003cli\u003eIntuitive \u0026amp; efficient: Refeyn’s software automatically controls the acquisition process and performs the mass analysis in minutes. You can intuitively interpret the mass distribution results without the need for any prior knowledge.\u003c\/li\u003e\n\u003cli\u003eMinimal setup required: A compact, bench-top instrument with minimal installation requirements. Measurements only require a few microliters of sample and clean sample-carrier slides.\u003c\/li\u003e\n\u003cli\u003eRapid, automated measurement of multiple samples\u003c\/li\u003e\n\u003cli\u003eHighly reproducible data with a user-friendly software\u003c\/li\u003e\n\u003cli\u003eCompatible with TwoMP unit\u003c\/li\u003e\n\u003cli\u003eThe TwoMP Auto streamlines your workflow, with highly reproducible data \u0026amp; a user-friendly software\u003c\/li\u003e\n\u003cli\u003eThe TwoMP Auto delivers automated mass photometry\u003c\/li\u003e\n\u003cli\u003eAutomated mass characterization: A user-friendly software interface; Automation unit compatible with TwoMP; Ideal for screening and titration assays\u003c\/li\u003e\n\u003cli\u003eHighly reproducible data: Precision pipetting; Consistent measurements\u003c\/li\u003e\n\u003cli\u003eIntuitive operation: A user-friendly software interface; Automation unit compatible with TwoMP\u003c\/li\u003e\n\u003cli\u003eHighly reproducible results: The liquid-handling robot autonomously runs 24 measurements in *as little as 90 minutes (up to 180 minutes, depending on the protocol). A straightforward, repeated measurement of a sample containing the antibody IgG illustrates the precision of the automated mass photometer, which is reflected in the high degree of reproducibility of the resulting data (Fig. 1). Figure 1A shows histograms of 12 repeated measurements of an IgG sample made using automated mass photometry. Across the wells that contain the sample, the measured mass (Fig. 1B) and total counts (Fig. 1C) show low variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eProtein-protein interactions: Antigen-antibody interactions are a prime example of molecular systems that can be studied using mass photometry, which can be applied to determine binding affinities for mono- and multivalent interactions.\u003c\/li\u003e\n\u003cli\u003eDNA-protein interactions: Mass photometry can be used to characterise DNA-protein complexes, which are crucial in gene expression, replication and DNA repair. In this example, mass photometry not only allows detection of DNA binding but also provides information on how the oligomeric state of the protein changes upon DNA binding (Figure 2).\u003c\/li\u003e\n\u003cli\u003eProtein oligomerization: Many proteins adopt a particular oligomeric form under certain conditions. In Figure 1, mass photometry characterises four different proteins: protein A, beta-amylase, urease and thyroglobulin. Through this characterisation, these proteins showcase a range of behaviours – from purely monomeric (as in Protein A) to a dynamic equilibrium between multiple states.\u003c\/li\u003e\n\u003cli\u003eComplex formation: Ribosomes are macromolecular assemblies of protein and RNA, and are central sites for protein synthesis. Bacterial ribosomes (in this example from E. coli) are \u0026gt;2 MDa in size. Magnesium ions play a subtle yet important role in the assembly of intact (70S) complexes. In the absence of magnesium, E. coli ribosomes rapidly disassemble into their 30S and 50S subunits. Mass photometry allows us to monitor these processes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\u003ctable\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eTwoMP\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMass detection range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e30 kDa to 5 MDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSample concentration on instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e100 pM to 100 nM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSample volume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ea few microliters of sample\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eTwoMP Auto\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eThroughput\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e24 measurements in as little as 90 minutes (up to 180 minutes, depending on the protocol)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eIn-plate dilution options (software R1 2023)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1\/2, 1\/4, 1\/8, 1\/10, 1\/25, 1\/50, 1\/100, 1\/250, 1\/500, 1\/1000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eTwoMP MassFluidix HC add-on\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSample concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eup to tens of micromolar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\u003ctd colspan=\"2\"\u003e\u003cstrong\u003eTwoMP Auto\u003c\/strong\u003e\u003c\/td\u003e\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCompatible instrument\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTwoMP (existing TwoMP and SamuxMP units can be upgraded)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/info.refeyn.com\/twomp-auto-product-data-sheet\" target=\"_blank\" rel=\"noopener\"\u003eTwoMP Auto product data sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/info.refeyn.com\/massferencep1-product-data-sheet\" target=\"_blank\" rel=\"noopener\"\u003eMassFerence P1 product data sheet\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/info.refeyn.com\/l\/983761\/2025-02-10\/53l85\/983761\/1739186792Bj5PnWlw\/Refeyn_BioanalyticsWithMassPhotometry.pdf\" target=\"_blank\" rel=\"noopener\"\u003eRefeyn Bioanalytics With Mass Photometry (brochure)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/refeyn.com\/eshop\/massfluidix-hc-sample-preparation-plate\" target=\"_blank\" rel=\"noopener\"\u003eMassFluidix™ HC – Sample Preparation Plate\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/refeyn.com\/eshop\/massglass-uc-sample-prep-kit\" target=\"_blank\" rel=\"noopener\"\u003eMassGlass™ UC – Sample Prep Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/refeyn.com\/eshop\/mp-alignment-kit\" target=\"_blank\" rel=\"noopener\"\u003eMP – Alignment Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/refeyn.com\/eshop\/massference-p1-calibrant\" target=\"_blank\" rel=\"noopener\"\u003eMassFerence™ P1 Calibrant\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/refeyn.com\/eshop\/massfluidix-hc-sample-prep-kit\" target=\"_blank\" rel=\"noopener\"\u003eMassFluidix™ HC – Sample Prep Kit\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Refeyn","offers":[{"title":"Refeyn TwoMP","offer_id":46733020332111,"sku":"REFEYN-TWOMP","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Refeyn TwoMP Auto","offer_id":46733020364879,"sku":"REFEYN-TWOMP-AUTO","price":0.0,"currency_code":"ILS","in_stock":true},{"title":"Refeyn TwoMP MassFluidix HC add on","offer_id":46733020397647,"sku":"REFEYN-MASSFLUIDIX-HC","price":0.0,"currency_code":"ILS","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0712\/0748\/5519\/files\/TwoMP-Open-Transparent-Background-V2.jpg?v=1788947381"}],"url":"https:\/\/www.danyel.co.il\/collections\/protein-research.oembed","provider":"danyel biotech","version":"1.0","type":"link"}